Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10

Pehlivanli, Faruk; Aydın, Oktay; Karaca, Gökhan; Aydın, Gülçin; Devrim, Tuba; Bulut, Huri; Bakar, Bülent; Daphan, Çağatay Erden

doi:10.3390/jcm7070161

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…Intra-abdominal adhesions were evaluated by visual scoring according to Houston and Rotstein. [ 24 ] Anastomosis burst pressure was measured according to the method described by Pehlivanlı et al[ 25 ] The excised colon was longitudinally cut into two parts. One portion was fixed in 10% neutral buffered formalin for 24–48 h; other portion was stored at −80°C for further biochemical analysis.…”

Section: Methodsmentioning

confidence: 99%

Effects of adipose tissue-derived mesenchymal stem cells and/or sildenafil citrate in experimental colon anastomosis model

Demir¹

2021

Ulus Travma Acil Cerrahi Derg

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BACKGROUND: This study aimed to evaluate the healing effects of adipose tissue-derived mesenchymal stem cells (AT-MSC) and sildenafil citrate alone or in combination of colon anastomosis experimental model. METHODS: A total of 40 female Wistar rats were randomly distributed to four groups: Control (without any intervention post-anastomosis), stem cell (AT-MSC injection on the anastomosis site), SIL (oral gavage of 10 mg/kg sildenafil citrate), and stem cell + SIL (AT-MSC injection and oral administration of sildenafil citrate) groups. Rats were euthanized 5 days post-anastomosis. Intra-abdominal adhesion status and anastomotic burst pressure were measured to assess anastomotic healing. Hydroxyproline and TNF-α level, neutrophil leukocyte infiltration, epithelial regeneration, and necrosis in the anastomosis tissue were examined. RESULTS: Anastomosis leakage and anastomosis burst pressure were not different among the groups. Treatment with sildenafil, stem cell, and stem cell + SIL reduced the degree of perianastomotic adhesions compared to control (p<0.05). A significant increase was noted in hydroxyproline in the stem cell and stem cell + SIL groups (p=0.001). AT-MSC injection alone or in combination with sildenafil citrate reduced the TNF-α concentration at the anastomosis site (p=0.001). Histopathological examination revealed that all treatments enhanced the clearance of the necrotic debris, reduced leukocytes infiltration, and accelerated the retraction of anastomosed ends except control (p=0.001). Epithelial regeneration was more pronounced in the stem cell group than other groups (p=0.001). Macrophage density was lower in groups treated with the SIL or stem cell groups than the control and stem cell + SIL groups (p=0.001). CONCLUSION: Sildenafil citrate and/or AT-MSC in the anastomosed rats promoted the anastomosis healing that was more pronounced in groups receiving stem cell injections.

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Section: Methodsmentioning

confidence: 99%

Effects of adipose tissue-derived mesenchymal stem cells and/or sildenafil citrate in experimental colon anastomosis model

Demir¹

2021

Ulus Travma Acil Cerrahi Derg

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“…Therefore, anastomotic leak has been one of the most investigated topics in colorectal surgery 11 , 24 . In the literature, there are experimental studies on anastomotic healing which involve methods such as reducing the number of sutures, dexpanthenol, coenzyme Q10, krill oil, and fish oil 3 , 6 , 9 , 17 . The indication for surgery and many factors related to the operated patient is also as effective as the technique used in colorectal surgery.…”

Section: Introductionmentioning

confidence: 99%

Different Surgical Methods in Colon Anastomosis: Experimental Study

Çantay

Aydin

Özaydin

et al. 2022

ABCD, arq. bras. cir. dig.

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- BACKGROUND: Although many methods have been defined for colonic anastomosis, anastomotic leak still remains important for sepsis control and successful healing. AIM: The purpose of this study was to compare the effects of conventional suture, polyglactin 910 mesh, and omental flap coverage on healing and anastomotic leak in experimental colonic anastomosis in rats. METHOD: This study was conducted on 18 Wistar rats and the animals were divided into three groups as follows: Group 1: primary suture group; Group 2: primary suture plus polyglactin 910 mesh group; and Group 3: primary suture plus omental flap coverage group. Groups were compared in terms of anastomotic bursting pressure, inflammation, fibroblastic activity, neovascularization, and collagen amount. RESULTS: There was a statistically significant difference in anastomotic bursting pressure between Groups 1 and 2 and between Groups 1 and 3 (p=0.004, p<0.05). There was a significant difference in fibroblastic activity between Groups 1 and 3 (p=0.011, p<0.05) and between Groups 2 and 3 (p=0.030, p<0.05). There was a significant difference in neovascularization and collagen between Groups 1 and 2 and between Groups 1 and 3 (p<0.05). CONCLUSION: This experimental study found that polyglactin 910 mesh and omental flap coverage for colocolic anastomoses improved the physical strength and healing of the anastomosis compared to conventional hand-stitched anastomoses. The polyglactin may be a safe alternative to 910 mesh in cases where the omental flap coverage cannot be used in the colonic anastomosis.

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“…The latter is one of the important factors affecting the healing process [3]. Ischemia in the anastomosis may occur due to arteriosclerotic vascular disease, advanced age, inflammatory vascular disease, vasoconstrictor drug use, and vascular network damage during the surgical manipulation [4,5].…”

Section: Introductionmentioning

confidence: 99%

The effect of carbon monoxide releasing molecule-2 (CORM-2) on healing of ischemic colon anastomosis in rats

Kayapinar¹,

Ercan

Baş³

et al. 2021

Turk J Med Sci

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Background/aim: Ischemia on the colon wall negatively affects healing of anastomosis. We were aimed to evaluate the effects of Carbon Monoxide Releasing Molecule-2 (CORM-2) on the healing of anastomosis in a rat model of the ischemic colon. Materials and methods:In this prospective study a total of 60 rats were randomly divided into three groups as colon transection and end-to-end anastomosis (Group I), colon transection and end-to-end anastomosis following the induction of ischemia (Group II), and colon transection and end-to-end anastomosis following the induction of ischemia and treated with daily intraperitoneal administration of CORM-2 (Group III). Each group was also divided into two equal subgroups as postoperative 3 rd and 7 th day. Postoperative healing of anastomoses was evaluated by anastomosis burst pressure (ABP), tissue biomarkers including hydroxyproline (HP), malondialdehyde (MDA), glutathione (GSH), and histopathological findings. Results:In the ischemic group treated with CORM-2, lower MDA and higher HP levels were observed in comparison to the untreated ischemic group at the 3 rd day. GSH and HP levels were higher and MDA levels was lower in the ischemic rats treated with CORM-2 than in the ischemic untreated rats at the 7 th day. In the ischemic group treated with CORM-2, the mucosal epithelial score decreased and the neoangiogenesis score increased compared to the untreated rats at 7 th day. Conclusion:In ischemic colon anastomosis, reduces cell destruction by suppressing the oxidative reaction and strengthening the antioxidative mechanisms of the cells. It also increases collagen formation, epithelial development and neoangiogenesis.

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Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10

Cited by 4 publications

References 27 publications

Effects of adipose tissue-derived mesenchymal stem cells and/or sildenafil citrate in experimental colon anastomosis model

Effects of adipose tissue-derived mesenchymal stem cells and/or sildenafil citrate in experimental colon anastomosis model

Different Surgical Methods in Colon Anastomosis: Experimental Study

The effect of carbon monoxide releasing molecule-2 (CORM-2) on healing of ischemic colon anastomosis in rats

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