Healing of Pseudomonas aeruginosa-infected wounds in diabetic db/db mice by weakly acidic hypochlorous acid cleansing and silver nanoparticle/chitin-nanofiber sheet covering

Kuwabara, Masahiro; Sato, Yoko; Takayama, T.; Nakamura, Shingo; Fukuda, Koichi; Murakami, Kaoru; Yokoe, Hidetaka; Kiyosawa, Tomoharu

doi:10.1016/j.wndm.2020.100183

Cited by 23 publications

(11 citation statements)

References 27 publications

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“…Spraying BiSCaO Water, which is colorless and transparent with a pH > 12.7, on a smooth metal or plastic surface, followed by drying, provides a white powder coating. Scanning electron microscope (SEM) images of dried powders of BiSCaO Water are shown in Figure 1-a,b, and cryo-SEM [22,28] images of BiSCaO Water are shown in Figure 1-c,d. After drying BiSCaO Water, the BiSCaO particles become larger (1-2 μm) compared to those in BiSCaO Water.…”

Section: Characterization Of Biscao Watermentioning

confidence: 99%

“…Daily wound cleaning with HClO solution (pH 6.5) for 12 days decreased the Pseudomonas aeruginosa (P. aeruginosa) bioburden of infected wounds in db/db diabetic mice but wound healing was delayed [18]. Limiting disinfection treatment with both HClO and chitin-nanofiber sheet-immobilized silver nanoparticles (CNFS/Ag NPs) to 3 days [19][20][21] may suppress these negative effects on wound repair [22]. We previously showed that treating P. aeruginosa-infected wounds on hairless rats with BiSCaO suspension (0.2 wt%, pH 12.3) once daily for 3 days and covering the wound with CNFS significantly decreased the P. aeruginosa bioburden and enhanced healing [23].…”

Section: Introductionmentioning

confidence: 99%

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Concentrated Bioshell Calcium Oxide (BiSCaO) Water Kills Pathogenic Microbes: Characterization and Activity

et al. 2020

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Bioshell calcium oxide (BiSCaO) exhibits deodorizing properties and broad microbicidal activity. In this study, we examined possible utility of BiSCaO Water for that purpose. BiSCaO Water was prepared by adding 10 wt% BiSCaO to clean water and gently collecting the supernatant in a bottle. The same volume of clean water was gently poured onto the BiSCaO precipitate and the supernatant was gently collected in a bottle; this process was repeated fifty times. The produced BiSCaO Water contained nanoparticles (about 400–800 nm) composed of smaller nanoparticles (100–200 nm), and was colorless and transparent, with a pH > 12.7. In vitro assays demonstrated that BiSCaO Water eliminated more than 99.9% of influenza A (H1N1) and Feline calicivirus, Escherichia coli such as NBRC 3972 and O-157:H7, Pseudomonas aeruginosa, Salmonella, and Staphylococcus aureus within 15 min. We compared BiSCaO Water with the other microbicidal reagents such as ethanol, BiSCaO, BiSCa(OH)2 suspensions, povidone iodine, NaClO, BiSCaO dispersion and colloidal dispersion with respect to deodorization activity and microbicidal efficacy. The results showed that BiSCaO Water was a potent reagent with excellent deodorization and disinfection activities against pathogenic bacteria and viruses (including both enveloped and nonenveloped viruses).

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Section: Characterization Of Biscao Watermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Concentrated Bioshell Calcium Oxide (BiSCaO) Water Kills Pathogenic Microbes: Characterization and Activity

et al. 2020

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“…It is known that daily cleaning of wounds with HClO solution (pH 6.5) decreases the Pseudomonas aeruginosa bioburden of infected wounds in db/db diabetic mice, but it delays wound healing [ 9 ]. To overcome the negative effects of HClO on wound healing, strategies such as the use of chitin-nanofiber sheet-immobilized silver nanoparticles (CNFS/Ag NPs) along with less HClO treatments have been investigated [ 19 , 20 , 21 , 22 , 23 ]. In this context, BiSCaO can remarkably disinfect wounds while maintaining healing; when P. aeruginosa -infected wounds on hairless rats were treated with a BiSCaO suspension (0.2 wt.%, pH 12.3) [ 24 ] or a BiSCaO-containing ointment [ 25 ] once daily for 3 days with covering the wounds with CNFS, wound healing was enhanced with significant decrease in the P. aeruginosa bioburden [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Safety of Concentrated Bioshell Calcium Oxide Water Application for Surface and Skin Disinfections against Pathogenic Microbes

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Immediately post-production, commercially available bioshell calcium oxide (BiSCaO) water is colorless, transparent, and strongly alkaline (pH 12.8), and is known to possess deodorizing properties and broad microbicidal activity. However, BiSCaO Water may represent a serious safety risk to the living body, given the strong alkalinity. This study aimed to investigate the safety of BiSCaO Water for use as an antiseptic/disinfectant despite concerns regarding its high alkalinity. The change over time in pH of BiSCaO Water was measured during air contact (stirring BiSCaO Water in ambient air). When sprayed on metal, plastic, wood piece, paper, and skin surfaces, the pH of BiSCaO Water decreased rapidly, providing a white powder coating upon drying. Scanning electron microscopy images, energy dispersive X-ray elemental mapping, and X-ray diffractograms showed that the dried powder residues of BiSCaO Water were composed primarily of calcium carbonate. These results suggested that BiSCaO Water is a potent reagent that may overcome the obstacles of being strongly alkaline, making this material appropriate for use in disinfection against pathogenic microbes.

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“…The mid-term use (three days) of AgNPs-incorporated chitin nanofiber sheet after wound bed cleansing with weakly acidic hypochlorous acid (pH 6.5) determined enhanced disinfection and significant healing efficiency in mice diabetic wounds infected with P. aeruginosa [ 428 ]. Hybrid hydrogels of thiolated CS and dextran grafted with maleic acid embedded with AgNPs were proposed as effective dressings for diabetic ulcers.…”

Section: Silver Nanoparticles For Wound Carementioning

confidence: 99%

An Updated Review on Silver Nanoparticles in Biomedicine

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Silver nanoparticles (AgNPs) represent one of the most explored categories of nanomaterials for new and improved biomaterials and biotechnologies, with impressive use in the pharmaceutical and cosmetic industry, anti-infective therapy and wound care, food and the textile industry. Their extensive and versatile applicability relies on the genuine and easy-tunable properties of nanosilver, including remarkable physicochemical behavior, exceptional antimicrobial efficiency, anti-inflammatory action and antitumor activity. Besides commercially available and clinically safe AgNPs-based products, a substantial number of recent studies assessed the applicability of nanosilver as therapeutic agents in augmented and alternative strategies for cancer therapy, sensing and diagnosis platforms, restorative and regenerative biomaterials. Given the beneficial interactions of AgNPs with living structures and their nontoxic effects on healthy human cells, they represent an accurate candidate for various biomedical products. In the present review, the most important and recent applications of AgNPs in biomedical products and biomedicine are considered.

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Healing of Pseudomonas aeruginosa-infected wounds in diabetic db/db mice by weakly acidic hypochlorous acid cleansing and silver nanoparticle/chitin-nanofiber sheet covering

Cited by 23 publications

References 27 publications

Concentrated Bioshell Calcium Oxide (BiSCaO) Water Kills Pathogenic Microbes: Characterization and Activity

Concentrated Bioshell Calcium Oxide (BiSCaO) Water Kills Pathogenic Microbes: Characterization and Activity

Safety of Concentrated Bioshell Calcium Oxide Water Application for Surface and Skin Disinfections against Pathogenic Microbes

An Updated Review on Silver Nanoparticles in Biomedicine

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