Healing of skin wounds in the African catfish Clarias gariepinus

Abstract

The African catfish Clarias gariepinus was used as a model for wound healing and tissue regeneration in a scale-less fish. A temporal framework of histological and cell proliferation markers was established after wound induction in the dorsolateral cranial region, by removing the epidermal and dermal layers, including stratum adiposum (SA). Wound closure and epidermis formation was initiated within 3 h post-procedure (hpp) with migration and concomitant proliferation of epidermal cells from the wound borders. The wound was covered by this primary epidermal front 12 hpp and fusion of the opposing epidermal fronts occurred within 24 hpp. Attachment of the newly formed epidermal layer to the underlying dermis was observed 48 hpp concomitant with a second wave of cell proliferation at the wound edge. Normal epidermal thickness within the wound was achieved 72 hpp. Formation of a basement membrane occurred by 120 hpp with concomitant emergence of the SA from the wound borders. Wound healing in C. gariepinus skin involved closure of the wound and re-epithelization through cell migration with a single wave of early cell proliferation not documented in other species. Furthermore, covering of the wound by epithelium as well as the reappearance of the basement membrane and SA occurred sooner than in other fish species.

Histopathology and Wound Healing in Oxytetracycline Treated Oreochromis niloticus (L.) Against Aeromonas hydrophila Intramuscular Challenge

“…The formation of black scar in the ulcerated area within 3 days of OTC therapy indicated the closure of the wound by the layers of epidermal cells. Darkened areas surrounding the wound in 3-6 days of therapy may be due to increased number of melanocytes, thereby, suggesting an increased melanocytes activity after the injury [28,29]. Within 6-10 days of OTC therapy, regeneration of muscle tissue with the closure of wounds, disappearance of black scar, onset of dermal fibrous tissue growth and the development of skin at the ulcerated scar region was obvious.…”

Wound healing in Piaractus mesopotamicus supplemented with chromium carbochelate and Saccharomyces cerevisiae

“…The skin of fish is the main defense organ against environmental hazards (BANERJEE;MITTAL, 1999) and its ability to quickly replace damaged or dead cells is essential to prevent blood loss, maintain the integrity of the surrounding tissue and prevent pathogen invasion (GUERRA et al, 2008;RICHARDSON et al, 2013).…”

“…Wound healing is a complex process that consists of several events, which can overlap each other, including the formation of hemorrhagic clots, inflammation, formation of granulation tissue, angiogenesis, re-epithelialization, wound contraction, and extracellular matrix remodeling (GUERRA et al, 2008;SCHMIDT et al, 2016).…”

Population dynamics, diet and ecotoxicology of the Great Cormorant Phalacrocorax carbo (Linnaeus, 1758) in the Carska bara marshland

“…Ove pojave su karakteristične za sporije zarastanje rana, često na niskim temperaturama (Silva et al, 2005). Silva et al, (2005) su izmerili i povećanu debljinu epitela, karakterističnu za proliferisan epitel, dok su Guerra et al, (2008) opisali sloj epidermisa koji se pojavljuje odmah nakon povrede i koji je obično tanji nego kod neoštećene kože. Kod nekih uzoraka u ovoj studiji epidermis je nedostajao ili se video sloj epidermisa koji je tanji od normalnog sloja (Sl.…”

“…Sporija revaskularizacija u dermisu je i očekivana u ovakvom tipu povreda s obzirom na to da trauma koju je riba doživela utiče na brzinu obnavljanja mreže krvnih sudova (Ritchie et al, 2005). Povećan broj melanocita je u skladu sa literaturnim podacima koji sugerišu povećanje aktivnosti melanocita nakon povreda (Iger & Abraham, 1990;Banerjee & Mittal, 1999;Guerra et al, 2008;Ottesen & Amin, 2011). Ovo se verovatno dešava zbog adaptacije riba na novonastale uslove i zaštite od sunčevog zračenja (Fabacher & Little, 1998).…”