Heart-type fatty acid binding protein — involvement in growth inhibition and differentiation

Börchers, Torsten; Hohoff, Carsten; Buhlmann, Carsten; Spener, Friedrich

doi:10.1016/s0952-3278(97)90496-8

Cited by 38 publications

(26 citation statements)

References 33 publications

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“…MDGI was shown to display 95% homology with the heart fatty acid -binding protein (33), a member of a family of proteins thought to be involved in cell signaling, growth inhibition, and differentiation (34). MDGI is a nuclear protein that is maximally expressed in terminally differentiated mammary tissue (35) and has inhibitory activity on the growth of different breast cancer cell lines (36), reducing the transcriptional expression of c-fos, c-myc, and c-ras and suppressing the mitogenic effects of EGF (37).…”

Section: Hrga 1-241 Interacts With Nuclear Proteinsmentioning

confidence: 99%

Heregulins Implicated in Cellular Functions Other Than Receptor Activation

Breuleux¹,

Schoumacher²,

Rehn³

et al. 2006

Molecular Cancer Research

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Heregulins (HRG) are known as soluble secreted growth factors that, on binding and activating ErbB3 and ErbB4 cell surface receptors, are involved in cell proliferation, metastasis, survival, and differentiation in normal and malignant tissues. Previous studies have shown that some HRG1 splice variants are translocated to the nucleus. By investigating the subcellular localization of HRGA 1-241 , nuclear translocation and accumulation in nuclear dot-like structures was shown in breast cancer cells. This subcellular distribution pattern depends on the presence of at least one of two nuclear localization sequences and on two domains on the HRG construct that were found to be necessary for nuclear dot formation. Focusing on the nuclear function of HRG, a mammary gland cDNA library was screened with the mature form of HRGA in a yeast two-hybrid system, and coimmunoprecipitation of endogenous HRG was done. The data reveal positive interactions of HRGA 1-241 with nuclear factors implicated in different biological functions, including transcriptional control as exemplified by interaction with the transcriptional repressor histone deacetylase 2. In addition, HRGA 1-241 showed transcriptional repression activity in a reporter gene assay. Furthermore, a potential of HRG proteins to form homodimers was reported and the HRG sequence responsible for dimerization was identified. These observations strongly support the notion that HRG1 splice variants have multifunctional properties, including previously unknown regulatory functions within the nucleus that are different from the activation of ErbB receptor signaling.

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Section: Hrga 1-241 Interacts With Nuclear Proteinsmentioning

confidence: 99%

Heregulins Implicated in Cellular Functions Other Than Receptor Activation

Breuleux¹,

Schoumacher²,

Rehn³

et al. 2006

Molecular Cancer Research

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“…It has been suggested that FABPs are concerned with mitochondrial and peroxisomal -oxidation, gene transcription and cell growth/ differentiation, and incorporation of fatty acids into triglyceride (Veerkamp & van Moerkerk. 1993, Glatz et al 1995, Borchers et al 1997, Zanotti 1999.…”

Section: Introductionmentioning

confidence: 99%

Fenofibrate improves insulin sensitivity in connection with intramuscular lipid content, muscle fatty acid-binding protein, and beta-oxidation in skeletal muscle

Furuhashi¹,

Ura²,

Murakami³

et al. 2002

Journal of Endocrinology

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We investigated the effect of fenofibrate, a peroxisome proliferator-activated receptor-agonist, on insulin sensitivity including lipid metabolism in skeletal muscle. Six-week-old male Sprague-Dawley rats were divided into two groups: those fed a standard chow (control) or a fructose-rich chow (fructose-fed rats (FFRs)) for 6 weeks. FFRs were treated either with a vehicle or with 30 mg/kg per day of fenofibrate for the last 2 weeks. Insulin sensitivity (M-value) was estimated by the euglycemic hyperinsulinemic glucose clamp method. Fatty acidbinding protein (FABP) in skeletal muscle was measured by ELISA, and the expression of FABP mRNA was analyzed by semi-quantitative RT-PCR. The serum and muscle triglyceride (sTG and mTG) levels and the activity of 3-hydroxyacyl-CoA dehydrogenase (HADH), a -oxidation enzyme, in muscle were also determined. FFRs showed a lower M-value and higher blood pressure, sTG and mTG than did the control group. The mTG was correlated positively with sTG and negatively with the M-value. Fenofibrate treatment for 2 weeks did not change blood pressure but significantly improved the M-value, sTG and mTG. FABP content and mRNA in the soleus muscle were significantly elevated in FFRs compared with those in the control group. Fenofibrate treatment further increased FABP. The HADH activity was comparable between the control group and FFRs, but significantly increased by fenofibrate treatment. These results suggest that fenofibrate improves insulin sensitivity not only by lowering serum lipids and subsequent influx of fatty acids into muscles but also by reducing intramuscular lipid content via further induction of FABP and stimulation of -oxidation in muscles.

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“…FABPs comprise a well-established family of cytoplasmic hydrophobic ligand binding proteins and are thought to be involved in lipid metabolism by binding and intracellular transport of long-chain fatty acids. However, from other studies on role for FABPs in cell signaling, growth inhibition and differentiation has also been implied (12,(16)(17). In particular, H-FABP and B-FABP are abundantly expressed in differentiated mammary gland.…”

Section: A-2 Fatty Acid Binding Protein (Fabp)mentioning

confidence: 98%

An Experimental Testbed for Tactical Command and Control

Davis¹,

Fu²,

Sarnacki³

et al. 2007

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. REPORT DATE (DD-MM-YYYY) 01-08-2005 REPORT TYPE Annual Summary DATES COVERED (From PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBERLong Island Jewish Medical Center Lake Success, NY 11042 SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S) U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012 SPONSOR/MONITOR'S REPORT NUMBER(S) DISTRIBUTION / AVAILABILITY STATEMENTApproved for Public Release; Distribution Unlimited SUPPLEMENTARY NOTES ABSTRACTA mammary derived growth inhibitor related gene (MRG) was previously identified and characterized. MRG induces differentiation of mammary epithelial cells in vitro and its expression is associated with mammary differentiation. Overexpression of MRG in human breast cancer cells induced differentiation with changes in cellular morphology and a significant increase in the production of lipid droplets. Treatment of mouse mammary gland in organ culture with MRG protein resulted in a differentiated morphology and stimulation of β-casein expression. To further define the role of MRG on mammary differentiation, a MRG transgenic mice model under the control of MMTV promoter was established and investigated. While there was no lobulo-alveolar structure in control virgin mice, expression of MRG transgene in the mammary gland resulted in the formation of alveolar-like structure. Consistent with the morphological change, expression of MRG also increased milk protein β-casein expression in the gland. Our results suggest that MRG is a candidate mediator of the differentiating effect of pregnancy on breast epithelial cells.

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Heart-type fatty acid binding protein — involvement in growth inhibition and differentiation

Cited by 38 publications

References 33 publications

Heregulins Implicated in Cellular Functions Other Than Receptor Activation

Heregulins Implicated in Cellular Functions Other Than Receptor Activation

Fenofibrate improves insulin sensitivity in connection with intramuscular lipid content, muscle fatty acid-binding protein, and beta-oxidation in skeletal muscle

An Experimental Testbed for Tactical Command and Control

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