Heartwood of Secang (CAESALPINIA SAPPAN L.) Ethanolic Extract Show Selective Cytotoxic Activities on T47D and Widr Cells But Not on Hela Cells

Abstract

The present study investigate the selectivity of heartwood of secang ethanolic extract (SEE) on T47D breast cancer cells, WiDr colon cancer cells, and HeLa cervical cancer cells, compared to Vero normal epithelial cells. The cytotoxic effect was evaluated by using MTT assay with 24-hour treatment to get IC50 values. Selectivity was evaluated by using selectivity index (SI). SEE had a potent cytotoxic activity on T47D and WiDr cancer cells (IC50<100 µg/mL). IC50 value of HeLa cancer cells was observed on moderate cytotoxic (100<IC50<1000 µg/mL). SEE demonstrated more selective to T47D and WiDr than Vero cells (SI>3), while in HeLa cells is not selective (SI<3). This result indicating its potential of Caesalpinia sappan as a chemopreventive agent in cancer therapy.

The Viability Test of Sappan Wood (Caesalpinia Sappan L.) Ethanol Extract in the H9c2 Cell Line

“…Previous SWEE in vivo toxicity tests using Wistar rats showed no toxic effects or allergic reactions, and no rats died until the SWEE dose reached 5000 mg/kg [11]. Furthermore, SWEE toxicity testing was also carried out in vitro on several cancer cells to test their toxicity on the cancer cells themselves, for example, in breast, cervical, and colon cancer cells [12,13]. In contrast with these previous studies, our study used the H9C2 rat cardiomyocytes cell line because we wanted to test the toxicity of SWEE in normal cells, including cardiomyocytes.…”