Heat-driven size reduction of biodegradable polyelectrolyte multilayer hollow capsules assembled on CaCO3 template

Trushina, Daria B.; Букреева, Т. В.; Bоrodinа, Tatyana N.; Belovа, Daria; Belyakov, S. A.; Antipina, Maria N.

doi:10.1016/j.colsurfb.2018.06.033

Cited by 41 publications

(49 citation statements)

References 53 publications

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“…In order to ensure the complete entrapment within the capsule lumen, the capsules were shrunk via heat-treatment. Heating multilayer systems typically results in the annealing of the polymer multilayers [77,78] and the closure of pores and defects present in the capsule wall due to compaction [79], resulting in an impermeable wall for DOX. This method has shown to dramatically reduce capsule permeability and enhance retention after shrinkage.…”

Section: Mechanism Of Lmw Drug Loadingmentioning

confidence: 99%

Encapsulation of Low-Molecular-Weight Drugs into Polymer Multilayer Capsules Templated on Vaterite CaCO3 Crystals

2020

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Polyelectrolyte multilayer capsules (PEMCs) templated onto biocompatible and easily degradable vaterite CaCO3 crystals via the layer-by-layer (LbL) polymer deposition process have served as multifunctional and tailor-made vehicles for advanced drug delivery. Since the last two decades, the PEMCs were utilized for effective encapsulation and controlled release of bioactive macromolecules (proteins, nucleic acids, etc.). However, their capacity to host low-molecular-weight (LMW) drugs (<1–2 kDa) has been demonstrated rather recently due to a limited retention ability of multilayers to small molecules. The safe and controlled delivery of LMW drugs plays a vital role for the treatment of cancers and other diseases, and, due to their tunable and inherent properties, PEMCs have shown to be good candidates for smart drug delivery. Herein, we summarize recent progress on the encapsulation of LMW drugs into PEMCs templated onto vaterite CaCO3 crystals. The drug loading and release mechanisms, advantages and limitations of the PEMCs as LMW drug carriers, as well as bio-applications of drug-laden capsules are discussed based upon the recent literature findings.

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Section: Mechanism Of Lmw Drug Loadingmentioning

confidence: 99%

Encapsulation of Low-Molecular-Weight Drugs into Polymer Multilayer Capsules Templated on Vaterite CaCO3 Crystals

2020

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“…We have previously determined a temperature treatment profile leading to size reduction and tightening of PMC capsules at 90 • C for 60 min [36]. In this case, to ensure the stability and biological efficacy of Cholosens, we lowered the temperature to 80 • C while keeping the treatment duration unchanged.…”

Section: Temperature-induced Morphological Changes In the [Ds/pagr] 4mentioning

confidence: 99%

“…As such, the encapsulation of low molecular weight, water-soluble compounds has not been attempted using the classic loading methods. In a series of publications [31,36,46], we extended this concept by bringing into consideration the ionic charge compensations occurring within the multilayer infrastructure [47]. There are two types of charge compensations in the PMC network: one that is formed by the interaction of the multilayer polyelectrolytes (we define it as "intraneous" compensation); the other one is the "extraneous" compensation held by the original counterions coming with the individual polyelectrolytes before forming the PMC.…”

Section: Introductionmentioning

confidence: 99%

“…PMC have been developed as a universal delivery platform for many essential biologically active molecules, such as growth factors [ 16 , 17 , 18 ], antigens [ 19 ], unspecific components of the immune system [ 20 ], enzymes [ 21 , 22 , 23 , 24 ], DNA [ 25 , 26 ], RNA [ 27 , 28 , 29 ], and anticancer drugs [ 30 , 31 ], providing multiple options for targeting and controlled release by remote and local physical, chemical, and biological stimuli [ 32 , 33 ]. Advancements in the miniaturization of vaterite templates and their respectively assembled PMC [ 34 , 35 , 36 ] have eased a long-held concern about the ability of such capsules to penetrate tissues and cells for delivery of drugs via intravenous injections. PMC with the size of ~250 nm were internalized by macrophages and epithelial cells of the lungs and liver with efficacy higher than 75% when administered in mice through an injection into the tail vein [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Bioeffects of Polyelectrolyte Multilayer Microcapsules Post-Loaded with Water-Soluble Cationic Photosensitizer

et al. 2020

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Microencapsulation and targeted delivery of cytotoxic and antibacterial agents of photodynamic therapy (PDT) improve the treatment outcomes for infectious diseases and cancer. In many cases, the loss of activity, poor encapsulation efficiency, and inadequate drug dosing hamper the success of this strategy. Therefore, the development of novel and reliable microencapsulated drug formulations granting high efficacy is of paramount importance. Here we report the in vitro delivery of a water-soluble cationic PDT drug, zinc phthalocyanine choline derivative (Cholosens), by biodegradable microcapsules assembled from dextran sulfate (DS) and poly-l-arginine (PArg). A photosensitizer was loaded in pre-formed [DS/PArg]4 hollow microcapsules with or without exposure to heat. Loading efficacy and drug release were quantitatively studied depending on the capsule concentration to emphasize the interactions between the DS/PArg multilayer network and Cholosens. The loading data were used to determine the dosage for heated and intact capsules to measure their PDT activity in vitro. The capsules were tested using human cervical adenocarcinoma (HeLa) and normal human dermal fibroblast (NHDF) cell lines, and two bacterial strains, Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. Our results provide compelling evidence that encapsulated forms of Cholosens are efficient as PDT drugs for both eukaryotic cells and bacteria at specified capsule-to-cell ratios.

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“…Thus, many PEMC systems have difficulty retaining smaller biomolecules. To overcome this, the multilayer pore size is controlled [ 201 , 202 ]. The smaller the pore size, the better the PEMC is at retaining smaller biomolecules.…”

Section: Problems Associated With Pemcsmentioning

confidence: 99%

Polyelectrolyte Multilayer Capsule (PEMC)-Based Scaffolds for Tissue Engineering

et al. 2020

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Tissue engineering (TE) is a highly multidisciplinary field that focuses on novel regenerative treatments and seeks to tackle problems relating to tissue growth both in vitro and in vivo. These issues currently involve the replacement and regeneration of defective tissues, as well as drug testing and other related bioapplications. The key approach in TE is to employ artificial structures (scaffolds) to support tissue development; these constructs should be capable of hosting, protecting and releasing bioactives that guide cellular behaviour. A straightforward approach to integrating bioactives into the scaffolds is discussed utilising polyelectrolyte multilayer capsules (PEMCs). Herein, this review illustrates the recent progress in the use of CaCO3 vaterite-templated PEMCs for the fabrication of functional scaffolds for TE applications, including bone TE as one of the main targets of PEMCs. Approaches for PEMC integration into scaffolds is addressed, taking into account the formulation, advantages, and disadvantages of such PEMCs, together with future perspectives of such architectures.

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Heat-driven size reduction of biodegradable polyelectrolyte multilayer hollow capsules assembled on CaCO3 template

Cited by 41 publications

References 53 publications

Encapsulation of Low-Molecular-Weight Drugs into Polymer Multilayer Capsules Templated on Vaterite CaCO3 Crystals

Encapsulation of Low-Molecular-Weight Drugs into Polymer Multilayer Capsules Templated on Vaterite CaCO3 Crystals

In Vitro Bioeffects of Polyelectrolyte Multilayer Microcapsules Post-Loaded with Water-Soluble Cationic Photosensitizer

Polyelectrolyte Multilayer Capsule (PEMC)-Based Scaffolds for Tissue Engineering

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