Heat-Killed Lactiplantibacillus plantarum LRCC5314 Mitigates the Effects of Stress-Related Type 2 Diabetes in Mice via Gut Microbiome Modulation

Abstract

The incidence of stress-related type 2 diabetes (stress-T2D), which is aggravated by physiological stress, is increasing annually. The effects of Lactobacillus , a key component of probiotics, have been widely studied in diabetes; however, studies on the effects of postbiotics are still limited. Here, we aimed to examine the mechanism through which heat-killed Lactiplantibacillus plantarum LRCC5314 (HK-LRCC5314) alleviates stress-T2D in a cold-induced stress-T2D C57BL/6 mouse model. HK-LRCC5314 markedly decreased body weight gain, adipose tissue (neck, subcutaneous, and epididymal) weight, and fasting glucose levels. In the adipose tissue, mRNA expression levels of stress-T2D associated factors (NPY, Y2R, GLUT4, adiponectin, and leptin) and pro-inflammatory factors (TNF-α, IL-6, and CCL-2) were also altered. Furthermore, HK-LRCC5314 increased the abundance of Barnesiella , Alistipes , and butyrate-producing bacteria, including Akkermansia , in feces and decreased the abundance of Ruminococcus , Dorea , and Clostridium . Thus, these findings suggest that HK-LRCC5314 exerts protective effects against stress-T2D via gut microbiome modulation, suggesting its potential as a supplement for managing stress-T2D.

Lactobacillus rhamnosus GG administration partially prevents diet-induced insulin resistance in rats: a comparison with its heat-inactivated parabiotic

“…Similar reductions in serum leptin levels were reported in a recent study using the same bacterial strain and a different experimental design, despite not having assessed further insulin resistance-related determinations. 48 Although some studies have reported that heat-killed bacteria administration may restore adiponectin and leptin levels in animals displaying glycaemic control impairments, 49 no such effects were observed in this study. This apparent discrepancy may well be due to the differences in the selected experimental conditions (bacterial strain and treatment length) and the animal model.…”

Multi-omic phenotyping reveals host-microbe responses to bariatric surgery, glycaemic control and obesity

“…Consistent with our observations in the current study, Ruminococcus and Parabacteroides have a higher relative abundance in T2D rodent models compared to animals without diabetes 61 , 62 and Ruminococcus has been shown to exert pro-inflammatory effects and also to promote pathogenesis in type 1 diabetes 63 . Similarly, lower relative concentrations of Barnsiella have been associated with T2D in rodent models 64 . In contrast, the role of Bacteroides is less clear with some studies reporting lower abundancy of Bacteroides in T2D 61 .…”

Heat-Killed Lacticaseibacillus paracasei Ameliorated UVB-Induced Oxidative Damage and Photoaging and Its Underlying Mechanisms

“…Secondly, PL down-regulated p-JNK and p-p38 (rather than p-ERK) protein expressions, which may be related to leptin. Nam et al (2022) found that heat-killed Lactiplantibacillus plantarum could decrease the leptin mRNA level in epididymal adipose tissue of mice [ 76 ]. It was reported that the reduction in leptin level down-regulated p-JNK and p-p38 (rather than p-ERK) protein expressions in LPS-stimulated kupffer cells of rats [ 77 ].…”