Heat-separation of normal human skin for epidermal and dermal prostaglandin analysis

Kassis, V.; Søndergaard, Jørgen

doi:10.1007/bf00409259

Cited by 62 publications

(34 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After in vitro skin penetration experiments epidermis was separated from dermis using dry heat separation method [41]. Separated layers were extracted with methanol, following bath sonication for 30 min.…”

Section: Hplc Analysis Of Human Skin Layers For Model Drug Contentmentioning

confidence: 99%

Studying the penetration of fatty acids into human skin by ex vivo TOF-SIMS imaging

et al. 2013

View full text Add to dashboard Cite

Fatty acids classified as chemical penetration enhancers (CPEs) might cause the fluidization and perturbation of stratum corneum (SC) lipid matrix. The penetration of oleic, linoleic, lauric and capric acids into human skin was studied by time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging and related to fatty acids enhancing effect on lipophilic model drug tolnaftate penetration into human epidermis and dermis ex vivo. Fatty acid enhancing effect on tolnaftate penetration into human skin was evaluated using Bronaugh-type flow-through diffusion cells. After in vitro penetration studies visualization and spatial localization of fatty acid molecules in human skin were performed using TOF-SIMS. Penetration of oleic, linoleic, lauric and capric acids into human skin was compared to the control skin sections by ion images and intensity profiles. Only oleic acid significantly (P<0.05) enhanced tolnaftate penetration into epidermis (enhancing ratio equal to 1.867). CPE might have no effect on model drug penetration enhancement, but might penetrate itself into the skin.

show abstract

Section: Hplc Analysis Of Human Skin Layers For Model Drug Contentmentioning

confidence: 99%

Studying the penetration of fatty acids into human skin by ex vivo TOF-SIMS imaging

et al. 2013

View full text Add to dashboard Cite

show abstract

“…20 Heating normal skin of the Yucatan micropig (Charles River Japan, Tokyo, Japan) at 60°C for 2 min resulted in the distinct separation of the stratum corneum from the skin tissue. The stratum corneum of Yucatan micropig was cut off with a biopsy (scalpel) (diameter, 6 mm; Kai Industries, Gifu, Japan) to prepare the SCE disk; and then washed three times with sterile physiological saline, as described previously.…”

Section: Preparation Of Sce Disk and Susceptibility Test With Sce Diskmentioning

confidence: 99%

Evaluation of the anti-Trichophyton activity of a prodigiosin analogue produced by γ-proteobacterium, using stratum corneum epidermis of the Yucatan micropig

Nakashima

Yamaguchi

Oda

et al. 2005

Journal of Infection and Chemotherapy

View full text Add to dashboard Cite

“…60°C heat applied for up to 2 min has long been used to separate human and animal skin along the epidermal-dermal junction [3]. This procedure is used to prepare skin membranes for permeation assessment.…”

Section: Mechanical (Tape Stripping) Injurymentioning

confidence: 99%

Relationship of Hairless Mouse Skin Surface Temperature to Wound Severity and Maturation Time

Govil

Flynn

et al. 2003

Skin Pharmacol Physiol

View full text Add to dashboard Cite

Skin surface temperature (SST) changes measured on live hairless mice are presented as a simple means of following wound healing. SST is generally determined by 3 factors which are the ambient temperature, the rate of water loss from the surface of the skin and the diffusion of thermal energy from the body’s core. The SST increase immediately after a burn injury reflects the amount of thermal energy absorbed by the skin surface. For burns and other injuries, the drop in SST following injury, but after thermal equilibrium has been established, provides an early indication of the degree of impairment of the skin’s barrier. Three kinds of mouse skin wounds, mechanical (tape stripping), thermal and chemical (phenol application), were investigated. SST nadirs ranged from 2.1 to 4.4°C with mild to full-thickness burns, respectively. Except for the earliest moments after wounding, striking parallels were noted between SST and transepidermal water loss profiles for these injuries. The SST profile over the full course of wound healing clearly indicates the severity of the injury, the stages of wound maturation and the time to complete skin healing.

show abstract

Heat-separation of normal human skin for epidermal and dermal prostaglandin analysis

Cited by 62 publications

References 11 publications

Studying the penetration of fatty acids into human skin by ex vivo TOF-SIMS imaging

Studying the penetration of fatty acids into human skin by ex vivo TOF-SIMS imaging

Evaluation of the anti-Trichophyton activity of a prodigiosin analogue produced by γ-proteobacterium, using stratum corneum epidermis of the Yucatan micropig

Relationship of Hairless Mouse Skin Surface Temperature to Wound Severity and Maturation Time

Contact Info

Product

Resources

About