1988
DOI: 10.1128/aem.54.10.2515-2520.1988
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Heat shock affects permeability and resistance of Bacillus stearothermophilus spores

Abstract: Heat shock of dormant spores of Bacillus stearothermophilus ATCC 7953 at 100 or 80°C for short times, the so-called activation or breaking of dormancy, was investigated by separating the resulting spores by buoyant density centrifugation into a band at 1.240 g/ml that was distinct from another band at 1.340 g/ml, the same density as the original spores. The proportion of spores at 1.240 g/ml became larger when the original dormant spores were heated for a longer period of time, but integument-stripped dormant … Show more

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Cited by 37 publications
(13 citation statements)
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“…Electron microscopy. The methods for electron microscopy were those described previously (4,5). Fixed, embedded spores were cut into thin sections and then stained with uranyl acetate and lead citrate.…”
Section: Methodsmentioning
confidence: 99%
“…Electron microscopy. The methods for electron microscopy were those described previously (4,5). Fixed, embedded spores were cut into thin sections and then stained with uranyl acetate and lead citrate.…”
Section: Methodsmentioning
confidence: 99%
“…Heat shocking is a recognized method of increasing the likelihood of spore germination. 29 A standard pour plating method with tryptic soy agar was used for culturing. Serial dilutions were performed, with each dilution plated in triplicate.…”
Section: Culturing and Analysismentioning
confidence: 99%
“…It may also result in reversible changes in the tertiary structure of one or more proteins controlling the dormant state of a spore . Activated and dormant spores of B. stearothennophilus can be separated by buoyant density centrifugation into separate bands of differing density (Beaman et al, 1988). That implies activated spores differ significantly from viable, dormant spores.…”
Section: Introductionmentioning
confidence: 99%