Heat shock elements are involved in heat shock promoter activation during tobacco seed maturation

Prändl, Ralf; Schöffl, Fritz

doi:10.1007/bf00020615

Cited by 29 publications

(23 citation statements)

References 23 publications

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“…This confirms preliminary indications of the functional role of HSE in developmental regulation from deletion analyses of plant sHS promoters (Coca et al, 1996;Prä ndl and Schö ffl, 1996). Our results also agree with similar inferences from the finding that synthetic HSE are capable of inducing chimeric gene expression in seeds from minimal heterolog-ous promoters (Prä ndl and Schö ffl, 1996).…”

Section: Role Of Hse In the Developmental Regulation Of Ha Hsp177 G4supporting

confidence: 91%

“…Deletion analysis in this system established that promoter sequences downstream of -83, containing most of the proximal HSE array, were sufficient to confer normal heat-induced expression to chimeric genes, as well as their developmental expression during zygotic embryogenesis. On the basis of these and similar results, the possible involvement of HSE/HSF in the developmental regulation of plant sHSP gene promoters in seeds has been proposed (Coca et al, 1996;Prä ndl and Schö ffl, 1996;Prä ndl et al, 1995). There are also indications that plant sHS promoters might be regulated during zygotic embryogenesis by pathways that are not necessarily dependent on HSE/HSF.…”

Section: Introductionmentioning

confidence: 68%

“…To date, only two plant sHS promoters and 5Ј-flanking sequences have been reported to confer to chimeric genes developmental regulation during zygotic embryogenesis: those from Gm hsp 17.3B (Prä ndl and Schö ffl, 1996;Prä ndl et al, 1995) and Ha hsp17.7 G4 (Coca et al, 1996). In both cases deletion analyses did not separate heat shock response from developmental regulation.…”

Section: Introductionmentioning

confidence: 98%

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Dual regulation of a heat shock promoter during embryogenesis: stage‐dependent role of heat shock elements

1998

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SummaryTransgenic tobacco expression was analysed of chimeric genes with point mutations in the heat shock element (HSE) arrays of a small heat shock protein (sHSP) gene from sunflower: Ha hsp17.7 G4. The promoter was developmentally regulated during zygotic embryogenesis and responded to heat stress in vegetative tissues. Mutations in the HSE affected nucleotides crucial for human heat shock transcription factor 1 (HSF1) binding. They abolished the heat shock response of Ha hsp17.7 G4 and produced expression changes that demonstrated dual regulation of this promoter during embryogenesis. Thus, whereas activation of the chimeric genes during early maturation stages did not require intact HSE, expression at later desiccation stages was reduced by mutations in both the proximal (-57 to -89) and distal (-99 to -121) HSE. In contrast, two point mutations in the proximal HSE that did not severely affect gene expression during zygotic embryogenesis, eliminated the heat shock response of the same chimeric gene in vegetative organs. Therefore, by site-directed mutagenesis, it was possible to separate the heat shock response of Ha hsp17.7 G4 from its developmental regulation. The results indicate the co-existence, in a single promoter, of HSF-dependent and -independent regulation mechanisms that would control sHSP gene expression at different stages during plant embryogenesis.

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Section: Role Of Hse In the Developmental Regulation Of Ha Hsp177 G4supporting

confidence: 91%

Section: Introductionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 98%

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Dual regulation of a heat shock promoter during embryogenesis: stage‐dependent role of heat shock elements

1998

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“…The observation of efficient transcription from different plant sHSP promoters in seeds, regardless of the presence, or absence, of high homology HSEs puts some constraints to current models explaining the developmental regulation of these genes (9,30). A general involvement of HSEs and of transacting factors similar to mammalian HSF1 does not fit the available data for the sunflower genes.…”

Section: Discussionmentioning

confidence: 94%

A Plant Small Heat Shock Protein Gene Expressed during Zygotic Embryogenesis but Noninducible by Heat Stress

Carranco

Almoguera

Jordano

1997

Journal of Biological Chemistry

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A small heat shock protein (sHSP) gene from sunflower, Ha hsp17.6 G1, showed expression patterns that differ from what is known for members of this gene family. The mRNAs of this gene accumulated in seeds during late desiccation stages of zygotic embryogenesis but not in response to heat shock in vegetative tissues. The failure to respond to heat shock was independent of the developmental stage after germination and shock temperature. Nuclear run-on analyses demonstrated that transcription from the Ha hsp17.6 G1 promoter is not induced by heat shock. This agrees with the presence, in this promoter, of sequences with little similarity to heat shock elements. Our results show an evolutionary divergence, in the regulation of plant sHSP genes, which has originated stress-responsive genes and nonresponsive members within this gene family. We discuss implications for mechanisms controlling the developmental regulation of sHSP genes in plants.One of the characteristics of the plant heat shock response is the synthesis of a large number of different, but evolutionarily related, polypeptides of 17-30 kDa (the sHSPs).1 In contrast, animals express only one to four sHSPs upon heat shock. The diversification in plants of heat-inducible sHSP genes could be a consequence of sesility; because plants cannot move away from heat, they would have evolved a battery of specialized "stress genes," the sHSPs. These are expressed in response to heat in all subcellular compartments and could allow plants to cope better with the stress conditions on site (for review, see Ref. 1). In animal and plant systems, heat shock genes encoding proteins of higher molecular weight, for example the HSP70s, have been shown to contain heat-inducible and noninducible members (2, 3). In the case of plant sHSPs the evidence for the existence of genes that are not induced by heat shock is weak and indirect, as it is based on the detection in seeds of sHSP isoforms that are different from the heat shockinduced polypeptides (4,5).In addition to being part of the heat shock response, some plant sHSP genes have been shown to be expressed at normal growth temperatures during zygotic embryogenesis (4 -7). Developmental regulation studies of plant sHSP genes are scarce. So far, only two plant sHSP promoters and 5Ј-flanking sequences have been reported to confer regulation to chimeric genes in maturing seeds: those from soybean Gm hsp17. 3B (8) and sunflower Ha hsp17.7 G4 (9). Initial studies have pointed to common control elements between the heat shock response and activation during embryogenesis. For example, the functional implication of HSEs in both processes is supported by results of deletion analysis (8, 9). Other observations point to the involvement in embryos of distinct control elements, for example, the effect of abi3 mutations on sHSP accumulation in Arabidopsis seeds (10). It is also clear that not all plant sHSP genes are developmentally regulated during embryogenesis, at least for the most systematically analyzed sunflower (9) and Arabidopsis ge...

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“…Specific class I and I1 smHSPs have been reported to accumulate, under temporal and spatial control, during embryo development in the seeds of several plant species (Hernández and Vierling, 1993;Coca et al, 1994;DeRocher and Vierling, 1994;zur Nieden et al, 1995). More recently, heat-shock elements have been shown to be involved in heat-shock promoter activation during tobacco seed maturation (Prandl and Schoffl, 1996).…”

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confidence: 99%

Purification and in Vitro Chaperone Activity of a Class I Small Heat-Shock Protein Abundant in Recalcitrant Chestnut Seeds

et al. 1997

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A 20-kD protein has been purified from cotyledons of recalcitrant (desiccation-sensitive) chestnut (Castanea sativa) seeds, where it accumulates at levels comparable to those of major seed storage proteins. This protein, termed Cs smHSP 1, forms homododecameric complexes under nondenaturing conditions and appears to be homologous to cytosolic class I small heat-shock proteins (smHSPs) from plant sources. In vitro evidence has been obtained that the isolated protein can function as a molecular chaperone: it increases, at stoichiometric levels, the renaturation yields of chemically denatured citrate synthase and also prevents the irreversible thermal inactivation of this enzyme. Although a role in desiccation tolerance has been hypothesized for seed smHSPs, this does not seem to be the case for Cs smHSP 1. We have investigated the presence of immunologically related proteins in orthodox and recalcitrant seeds of 13 woody species. Our results indicate that the presence of Cs smHSP 1-iike proteins, even at high levels, is not enough to confer desiccation tolerance, and that the amount of these proteins does not furnish a reliable criterion to identify desiccation-sensitive seeds. Additional proteins or mechanisms appear necessary to keep the viability of orthodox seeds upon shedding.

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Heat shock elements are involved in heat shock promoter activation during tobacco seed maturation

Cited by 29 publications

References 23 publications

Dual regulation of a heat shock promoter during embryogenesis: stage‐dependent role of heat shock elements

Dual regulation of a heat shock promoter during embryogenesis: stage‐dependent role of heat shock elements

A Plant Small Heat Shock Protein Gene Expressed during Zygotic Embryogenesis but Noninducible by Heat Stress

Purification and in Vitro Chaperone Activity of a Class I Small Heat-Shock Protein Abundant in Recalcitrant Chestnut Seeds

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