Heat Shock Protein 70 (Hsp70) in the Regulation of Platelet Function

Rigg, Rachel A.; McCarty, Owen J. T.; Aslan, Joseph E.

doi:10.1007/978-3-319-74715-6_14

Cited by 5 publications

(3 citation statements)

References 97 publications

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“…This pathway activates a conformational change in the extracellular domains of proteins such as fibrinogen or von Willebrand factor, and fibrinogens act as bridges between platelets to generate platelet aggregation 23 . Additionally, as noted above we also found the up-regulation of Hsp70, a specific chaperone that forms complexes with other co-chaperones in maintaining hemostasis and is associated with intracellular organization of signaling systems and platelet function 24 , and expression of Hsp70 is an endogenous mechanism by which living cells adapt to stress 25 . Moreover, Hsp70 is associated with the inside-out activation of integrin-αIIbβ3 and the activation of platelet aggregation as well as granule secretion and platelet formation under conditions of physiological shear 26 .…”

Section: Discussionsupporting

confidence: 74%

Platelet proteome reveals specific proteins associated with platelet activation and the hypercoagulable state in β-thalassmia/HbE patients

Chanpeng

Svasti

Paiboonsukwong

et al. 2019

Sci Rep

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A hypercoagulable state leading to a high risk of a thrombotic event is one of the most common complications observed in β-thalassemia/HbE disease, particularly in patients who have undergone a splenectomy. However, the hypercoagulable state, as well as the molecular mechanism of this aspect of the pathogenesis of β-thalassemia/HbE, remains poorly understood. To address this issue, fifteen non-splenectomized β-thalassemia/HbE patients, 8 splenectomized β-thalassemia/HbE patients and 20 healthy volunteers were recruited to this study. Platelet activation and hypercoagulable parameters including levels of CD62P and prothrombin fragment 1 + 2 were analyzed by flow cytometry and ELISA, respectively. A proteomic analysis was conducted to compare the platelet proteome between patients and normal subjects, and the results were validated by western blot analysis. The β-thalassemia/HbE patients showed significantly higher levels of CD62P and prothrombin fragment 1 + 2 than normal subjects. The levels of platelet activation and hypercoagulation found in patients were strongly associated with splenectomy status. The platelet proteome analysis revealed 19 differential spots which were identified to be 19 platelet proteins, which included 10 cytoskeleton proteins, thrombin generation related proteins, and antioxidant enzymes. Our findings highlight markers of coagulation activation and molecular pathways known to be associated with the pathogenesis of platelet activation, the hypercoagulable state, and consequently with the thrombosis observed in β-thalassemia/HbE patients.

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Section: Discussionsupporting

confidence: 74%

Platelet proteome reveals specific proteins associated with platelet activation and the hypercoagulable state in β-thalassmia/HbE patients

Chanpeng

Svasti

Paiboonsukwong

et al. 2019

Sci Rep

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“…More recent findings have indicated that the platelet HSP90/Hsp70 system has been hypothesized to modulate intracellular trafficking and multidrug resistance MDR4/ ABCC4 localization [17]. The HSP members additionally play essential roles in the assembly of actinomyosin filament, an important step in the change of platelet shape and spreading [18]. For instance, HSP27 has been implicated as a downstream p38 MAPK target in the assembly of the cytoskeleton, and phosphorylation of HSP27 is important for the secretion of platelet granules [19].…”

Section: Discussionmentioning

confidence: 99%

Heat shock protein-70 is elevated in childhood primary immune thrombocytopenia

Liu

2022

Eur J Med Res

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Background Immune thrombocytopenia (ITP) is an acquired autoimmune disorder characterized by the destruction of the platelets resulting from autoimmune recognition and subsequent attack. Heat shock proteins (HSPs) are directly associated with progression and pathogenesis in some specific autoimmune diseases. The aim of this study was to investigate the serum expression of HSP-70 in ITP children and healthy controls. Materials and methods A total of 86 children aged 1–6 years were enrolled in the study. The participants were divided into 20 newly diagnosed ITP (ndITP), 34 chronic ITP (cITP) patients and 32 healthy children. The white blood cells and platelet counts were determined and compared between the groups. HSP-70 serum levels were analyzed by sandwich ELISA. Data analysis was done using SPSS and the data variables assessment was done through histogram, probability plots and Shapiro–Wilk tests to determine normal distribution. Results The white blood cell counts were 8.9 (4.2–10.4) for new diagnosis ITP, 7.1(3.9–11.9) for the chronic ITP group and 7.0 (4.3–9.5) for the healthy controls. The platelet counts were significantly increased in the chronic ITP group, 83.5(31.7–297) compared to the ndITP group 27.4 (3.7–63.7), but significantly lower compared to the healthy controls 271(172–462) (P = 0.0009). There were significantly increased HSP-70 serum levels in cITP patients compared to the ndITP and the healthy group. In addition, there was a positive correlation between the serum HSP-70 level and the thrombocyte counts among the ITP children. Conclusions HSP-70 has a role in the progression of childhood ITP. Increased HSP-70 level is associated with the severity of childhood primary ITP.

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“…HSP60 and HSP90, which differ in molecular weight, position, and functions, are up-regulated as an aftermath of heat shock liability in fish, denoting that these may be harnessed as a stress marker (Rigg et al 2018). Still, emodin administration redounded in a drop in HSP60 and HSP90 gene expression compared to control, pointing that supplemented emodin levels of 25 and 50 mg kg -1 could not initiate HSP expression under free stress trials, resulting in M. rosenbergii failing to maintain cellular hemostasis.…”

Section: Figurementioning

confidence: 99%

Effect of emodin on growth performance, antioxidant status, intestinal morphology and heat shock protein gene expressions of freshwater prawn (Macrobrachium rosenbergii)

Tadese

Tesfaye

Tamiru

et al. 2022

J Fish

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The purpose of this study was to assess the effect of emodin on biochemical variables, antioxidant activity, heat shock protein gene expression and prawn intestinal morphology (Macrobrachium rosenbergii). Three feeds containing 0 mg kg–1 (T1, control group), 25 mg kg–1 (T2) and 50 mg kg–1 (T3) of emodin were formulated and fed for eight weeks. Alanine aminotransferase, total cholesterol and triglyceride contents were significantly lowered although glucose tended to increase following the emodin remedy. Superoxide dismutase variables in haemolymph were dramatically upgraded, whilst malondialdehyde and superoxide anion situations were significantly lowered when compared to control. Emodin supplementation had no influence on glutathione peroxidase contents. Emodin remedy dropped the expression of HSP60 and HSP90 genes in comparison to the control. In T2, the intestinal epithelial cells were complete, well-organised and tightly affixed to the basement membrane, in discrepancy to the slightly disunited basement membrane in the control group. The emodin-treated prawn individuals had a thicker and full intestinal morphology than the control groups. Supplementation with emodin at dosages of 25 mg kg–1 improved growth performance, blood biochemical, antioxidant capacity and immune gene expression.

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Heat Shock Protein 70 (Hsp70) in the Regulation of Platelet Function

Cited by 5 publications

References 97 publications

Platelet proteome reveals specific proteins associated with platelet activation and the hypercoagulable state in β-thalassmia/HbE patients

Platelet proteome reveals specific proteins associated with platelet activation and the hypercoagulable state in β-thalassmia/HbE patients

Heat shock protein-70 is elevated in childhood primary immune thrombocytopenia

Effect of emodin on growth performance, antioxidant status, intestinal morphology and heat shock protein gene expressions of freshwater prawn (Macrobrachium rosenbergii)

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