Heat shock protein 70 regulates the abscisic acid-induced antioxidant response of maize to combined drought and heat stress

Hu, Xiuli; Liu, Ruixia; Li, Yanhui; Wang, Wei; Tai, Fuju; Xue, Ruili; Chao-hai, LI

doi:10.1007/s10725-009-9436-2

Cited by 127 publications

(90 citation statements)

References 41 publications

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“…Therefore, the Hsp70 chaperones might play a broader role by participating in modulation of the expression of many downstream genes in signal transduction pathways (Nollen and Morimoto, 2002). Hu et al (2010) have suggested that HSP70 may play a crucial role in ABAinduced antioxidant defence of maize to the combination of drought and heat. Grigorova et al (2011) have observed an increase in Hsp70 in wheat plants under drought or heat stress.…”

Section: Discussionmentioning

confidence: 99%

Gene expression changes in response to drought stress in Ilex paraguariensis leaves

Acevedo¹,

Ruíz²,

Sansberro³

2016

Plant Omics

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Ilex paraguariensis St. Hil. is recognized as a shade-tolerant tree, cultivated in north-eastern Argentina, south-eastern Brazil, and Paraguay. Its leaves and young shoots are industrialised and; hence, being used to prepare a traditional tea-like infusion (mate-tea) with various medicinal benefits. Under the prevailing agro-climatic conditions in NE Argentina and with the occurrence of high temperatures and scarce rains during spring or summer, I. paraguariensis plants are subjected to water stress, reducing their yield. Differential Display was used to compare differences in gene expression between stressed and unstressed (control) potted plants. We found 52 differentially expressed transcripts (DETs), analysing banding patterns of Differential Display polyacrylamide gels, in which 38 DETs were induced, and 14 DETs were repressed in response to drought. Sequence comparisons revealed that 19% of the fragments showed no homologies, whereas 40.5 % of the total number of fragments had strong similarities with database entries of known functions. The other DETs belonged to unclassified proteins or genes with unknown functions. Interestingly, 12% of the DNA sequences were related to signal transduction, and 2% of the sequences were identified as transcription factors. Approximately, 12% of the annotated sequences were involved in stress tolerance and metal homeostasis, while 7% of the DETs were associated with photosynthesis. Other groups, representing 12%, included those involved in secondary metabolism, lignin biosynthesis, cell walls and RNA stabilization/chaperone activity. When integrated with physiological data, our results suggest that the transcriptional activity of some of the studied genes could be related to the degree of drought tolerance/susceptibility of I. paraguariensis.

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Section: Discussionmentioning

confidence: 99%

Gene expression changes in response to drought stress in Ilex paraguariensis leaves

Acevedo¹,

Ruíz²,

Sansberro³

2016

Plant Omics

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“…Furthermore, the induction of Hsps was higher in response to drought and heat shock compared with heat shock or drought individually [37]. In maize, Hsp70 and sHsps (sHsp17.2, sHsp17.4 and sHsp26) are responsive to a combination of drought and heat stress [12,38]. Four Hsp genes (Hsp17.8, Hsp26.3, Hsp70 and Hsp101b) in Triticum aestivum plants were monitored via one-step RT-PCR and SDS-PAGE analysis in plants subjected to water deprivation or high temperature individually and in combination.…”

Section: Function Of Oshsps Under Drought and Heat Stressmentioning

confidence: 99%

“…Hsp genes regulated by ABA have been reported in several plant species, such as maize, tobacco, wheat and rice [12,36,[42][43][44]. Results of studies with an ABA-deficient mutant indicated that endogenous ABA regulated Hsp70 synthesis in the leaves and roots of maize seedlings exposed to drought, heat, and combined drought and heat stress, which implied that ABA may improve plant tolerance to stress by increasing Hsp70 expression.…”

Section: Oshsp Genes May Be Involved In Aba-regulated Pathwaysmentioning

confidence: 99%

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Expression profile analysis of 9 heat shock protein genes throughout the life cycle and under abiotic stress in rice

Shu

et al. 2011

Chin. Sci. Bull.

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Plant heat shock proteins (Hsps) facilitate protein folding or assembly under diverse developmental and adverse environmental conditions. Nine OsHsps were identified in our previous study from a proteomic analysis of rice cv. IRAT 109 leaf samples at the seedling stage under drought stress. To obtain additional information on the 9 OsHsp genes, this study focused on an expression profile analysis at different development stages throughout the life cycle of rice, and under different abiotic stresses and phytohormone treatments. The 9 genes exhibited distinctive expression patterns in different organs or development stages. Five of the genes (OsHsp72. 90, OsHsp72.57, OsHsp71.18, OsHsp24.15 and OsHsp18.03) showed high expression in the endosperm, indicating that OsHsp genes may play important roles in rice seed development. All 9 OsHsps were up-regulated under heat, polyethylene glycol, and abscisic acid treatment, whereas salt stress caused up-regulation of 6 genes (OsHsp93. 04, OsHsp71.10, OsHsp71.18, OsHsp72.57, OsHsp24.15 and OsHsp18.03) and cold stress resulted in down-regulation of OsHsp93.04 and OsHsp72.57. These diverse expression profiles imply potential functional diversity of the Hsp gene family in rice.heat shock protein, drought, heat, real-time PCR, rice

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“…Genetic analysis has shown that Hsp101 is absolutely essential for thermal tolerance in Arabidopsis (Tonsor et al 2008), and this Hsp has been reported to play a role in preventing oxidative stress (Zhang et al 2009). Hsp70 is one of the major classes of chaperone molecules and is involved in a variety of tasks in eukaryotic cells, and increased Hsp70 synthesis results in a marked increase in stress tolerance (Hu et al 2010;Montero-Barrientos et al 2010). Although plants generate an array of high molecular weight Hsps, most of the translation capacity is devoted to the synthesis of the small Hsps (e.g., Hsp15.7-CI and Hsp17.4-CIII) which also play an important role in the acquisition of thermal tolerance (Yildiz and Terzi 2008).…”

mentioning

confidence: 99%

Ca2+-dependent protein kinases and their substrate HsfB2a are differently involved in the heat response signaling pathway in Arabidopsis

Kanchiswamy

Muroi

Maffei

et al. 2010

Plant Biotechnology

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Little is known about the mechanisms by which Ca 2ϩ -binding sensory proteins direct the plant heat shock (HS) response. Since two Ca 2ϩ -dependent protein kinases (CPK3 and CPK13) were recently shown to phosphorylate the heat shock transcription factor HsfB2a, we assessed in the current study whether these kinases are also involved in HS signal transduction, by monitoring the transcriptional profile of HS protein (Hsp) family genes in Arabidopsis Col-0 plants (WT) and the corresponding mutants. Both with and without HS, the gene transcript levels of Hsp70, Hsp101, Hsp17.4-CIII and Hsp15.7-CI were found to be lower in cpk3 and cpk13 mutants compared to WT, resulting in the impairment of basal thermotolerance in the mutants. To determine the in vivo function of CPKs, CPK3/13 and their substrate HsfB2a (heat shock transcription factor) were co-expressed as cofactors for the transient expression of a reporter (GUS) gene under the control of heat shock element (HSE) in Nicotiana benthamiana leaves. However, CPK3/13-phosphorylated HsfB2a did not function in the suppression/activation of HSE-promoted expression in the transient expression system. Implications for possible signal trafficking via CPKs and Hsfs are discussed.

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Heat shock protein 70 regulates the abscisic acid-induced antioxidant response of maize to combined drought and heat stress

Abstract: We investigated the interaction between heat shock protein 70 (HSP70) and abscisic acid (ABA)

Cited by 127 publications

References 41 publications

Gene expression changes in response to drought stress in Ilex paraguariensis leaves

Gene expression changes in response to drought stress in Ilex paraguariensis leaves

Expression profile analysis of 9 heat shock protein genes throughout the life cycle and under abiotic stress in rice

Ca2+-dependent protein kinases and their substrate HsfB2a are differently involved in the heat response signaling pathway in Arabidopsis

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