Heat stress induces expression of HSP genes in genetically divergent chickens

Cedraz, Haniel; Gromboni, Juliana Gracielle Gonzaga; García, Adriana; Filho, Ronaldo Vasconcelos Farias; Souza, Teillor Machado; Oliveira, Eduardo Ribeiro de; Oliveira, Elizângela Bonfim de; Nascimento, Carlos Souza do; Meneghetti, Camila; Wenceslau, Amauri Arias

doi:10.1371/journal.pone.0186083

Cited by 80 publications

(56 citation statements)

References 48 publications

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“…In contrast, some limitations of the present study include the relatively small sample size and the fact that the effect of TM on embryonic mortality and physiological parameters was not fully explored. However, the sample sizes utilized in the current study are in line with previously published reports [12,29].…”

Section: Discussionsupporting

confidence: 68%

“…Our findings show that CS decreased hepatic and splenic Hsp70 expression, respectively. Previous reports have shown that, during HS, Hsp70 expression was found to be lower in a commercial broiler breed (Cobb) compared to local ones (Caneluda and Peloco), and this high expression level was associated with resistance to elevated temperatures in the local breeds [12]. Moreover, Cobb and Hubbard chicks subjected to TM were found to express higher cardiac, hepatic, muscular and splenic levels of Hsp70 during post-hatch heat challenge [13,34,35,42].…”

Section: Discussionmentioning

confidence: 87%

“…Thermal stress can arise at many stages in the typical broiler's life, and climate change is endangering the welfare and stress levels of broilers around the world [11]. Upon exposure to such stress, broilers have been reported to exhibit a changed expression profile of certain stress-associated genes, including the HSF3 and Hsp70 genes [12,13]. The Hsp70 and HSF3 genes have been extensively investigated in the context of thermal stress in poultry, as both genes are considered to be a chief defense against heat challenge across many organisms [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

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HSF3 and Hsp70 Expression during Post-Hatch Cold Stress in Broiler Chickens Subjected to Embryonic Thermal Manipulation

Tarkhan

Saleh

Al‐Zghoul

2020

Veterinary Sciences

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Decades of selective breeding for commercial purposes have rendered the broiler chicken (Gallus gallus domesticus) highly susceptible to heat and cold stress. A multitude of studies have documented the effects of thermal manipulation (TM) on broiler thermotolerance during periods of post-hatch heat stress, but very few have focused on the effect of TM on a broiler’s ability to withstand cold stress. Therefore, the primary objective of the current study is to determine the effects of TM on the acquisition of thermotolerance in broilers via their expression of the stress-associated 70 kilodalton heat shock protein (Hsp70) gene and heat shock factor 3 (HSF3) gene. Briefly, Hubbard broiler embryos were subject to TM by increasing the incubation temperature to 39 °C and 65% relative humidity (RH) for 18 h daily, from embryonic days (ED) 10 to 18. Broilers were then exposed to cold stress by decreasing the room temperature to 16 °C during post-hatch days 32 to 37. After thermal challenge, broilers were euthanized and hepatic and splenic tissues were collected. Our results showed that TM decreased the hatchability rate and body temperature but improved the body weight gain. TM generally decreased the hepatic expression but did not change the splenic expression of HSF3 during cold stress. In contrast, both hepatic and splenic Hsp70 expression decreased during cold stress. The results of the present study may suggest that TM significantly affects a broiler’s genetic response to cold stress.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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HSF3 and Hsp70 Expression during Post-Hatch Cold Stress in Broiler Chickens Subjected to Embryonic Thermal Manipulation

Tarkhan

Saleh

Al‐Zghoul

2020

Veterinary Sciences

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“…Responses to heat vastly depend on the genetic adaptation of the individual [16] but also on environmental factors, such as intestinal microbiota [17]. Compromised genetic or environmental conditions result in poorer thermoregulation [18].…”

Section: Introductionmentioning

confidence: 99%

Splenic Gene Expression Signatures in Slow-Growing Chickens Stimulated in Ovo with Galactooligosaccharides and Challenged with Heat

Pietrzak

Dunisławska

Siwek

et al. 2020

Animals

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Galactooligosaccharides (GOS) that are delivered in ovo improve intestinal microbiota composition and mitigate the negative effects of heat stress in broiler chickens. Hubbard hybrids are slow-growing chickens with a high resistance to heat. In this paper, we determined the impact of GOS delivered in ovo on slow-growing chickens that are challenged with heat. The experiment was a 2 × 2 × 2 factorial design. On day 12 of incubation, GOS (3.5 mg/egg) was delivered into the egg (n = 300). Controls (C) were mock-injected with physiological saline (n = 300). After hatching, the GOS and C groups were split into thermal groups: thermoneutral (TN) and heat stress (HS). HS (30 °C) lasted for 14 days (days 36–50 post-hatching). The spleen (n = 8) was sampled after acute (8.5 h) and chronic (14 days) HS. The gene expression of immune-related (IL-2, IL-4, IL-6, IL-10, IL-12p40, and IL-17) and stress-related genes (HSP25, HSP90AA1, BAG3, CAT, and SOD) was detected with RT-qPCR. Chronic HS up-regulated the expression of the genes: IL-10, IL-12p40, SOD (p < 0.05), and CAT (p < 0.01). GOS delivered in ovo down-regulated IL-4 (acute p < 0.001; chronic p < 0.01), IL-12p40, CAT and SOD (chronic p < 0.05). The obtained results suggest that slow-growing hybrids are resistant to acute heat and tolerant to chronic heat, which can be supported with in ovo GOS administration.

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“…Among HSPs, HSP70 is most closely associated with cytoprotection and cellular thermoregulation (Beckham et al, 2008;Craig & Gross, 1991). Heat conditions increased the expression of HSP70 in a time-dependent manner in broilers (Yu & Bao, 2008) and native chickens (Cedraz et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary nutmeg oil on heat‐stress tolerance‐related parameters in Korean native chicken reared under hot temperature

Hartanto

Jee

et al. 2019

Animal Physiology Nutrition

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This study investigated the effect of dietary nutmeg oil (NO) on growth performance, blood parameters, lipid peroxidation and heat shock protein (HSP) 70 expression in Korean native chicken (KNC) reared under hot temperature. We allocated 273 meat‐type KNCs (Hanhyup‐3, 4‐week‐old, body weight [BW] = 539.93 ± 1.75 g) to the following three treatments with seven replicate pens (13 birds/pen) per treatment. Three treatment diets were as follows: (a) Control, basal diet without NO supplementation; (b) NO 250; and (c) NO 500, basal diet supplemented with 250 and 500 ppm NO respectively. Diets and water were provided ad libitum throughout the 6‐week feeding trial. During overall period (0–6 weeks), no differences (p > 0.05) were observed in BW gain (BWG), feed intake (FI) and feed conversion rate (FCR) among treatments. However, the FI at 0–3 weeks decreased (p < 0.05) quadratically with increasing NO levels. Most blood parameters did not differ (p > 0.05) among treatments, although the monocyte level of the NO 500 group was considerably lower (p > 0.05) than that of the other groups. Furthermore, dietary NO did not affect serum triglyceride, cholesterol, total protein, albumin, calcium, phosphorus and alanine aminotransferase (ALT) levels (p > 0.05); however, it linearly decreased serum aspartate aminotransferase (AST) level (p < 0.05). Additionally, serum malondialdehyde (MDA) concentration decreased (p < 0.05) and heart MDA concentration was lower (p = 0.08) with increasing dietary NO supplementation. After a 3‐hr heat (35°C) challenge, the rectal temperature (RT) reduced (p < 0.05) linearly with increasing NO levels. Dietary NO did not affect liver HSP70 (p > 0.05) gene expression. In conclusion, NO potentially enhanced the ability of chickens to alleviate heat stress. Furthermore, our findings suggest that lipid oxidation inhibition by dietary NO likely mediated the enhanced heat‐stress tolerance of the chickens.

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Heat stress induces expression of HSP genes in genetically divergent chickens

Cited by 80 publications

References 48 publications

HSF3 and Hsp70 Expression during Post-Hatch Cold Stress in Broiler Chickens Subjected to Embryonic Thermal Manipulation

HSF3 and Hsp70 Expression during Post-Hatch Cold Stress in Broiler Chickens Subjected to Embryonic Thermal Manipulation

Splenic Gene Expression Signatures in Slow-Growing Chickens Stimulated in Ovo with Galactooligosaccharides and Challenged with Heat

Effect of dietary nutmeg oil on heat‐stress tolerance‐related parameters in Korean native chicken reared under hot temperature

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