Heavy metals analysis, phytochemical, phytotoxic and anthelmintic investigations of crude methanolic extract, subsequent fractions and crude saponins from Polygonum hydropiper L

Ayaz, Muhammad; Junaid, Muhammad; Subhan, Fazal; Ullah, Farhat; Sadiq, Abdul; Ahmad, Sajjad; Imran, Muhammad; Kamal, Zul; Hussain, Sajid; Shah, Syed Majid

doi:10.1186/1472-6882-14-465

Cited by 56 publications

(44 citation statements)

References 28 publications

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“…Flavonoids are able to influence central nervous system activity (CNS) by binding to the benzodiazepine site on the GABA A receptor resulting in sedation, anxiolytic effects [11]. Several bioactive constituents have also been indicated from P. hydropiper with antihyperglycemic [12], antioxidant [13], antinociceptive [8], anti-inflammatory [14], cytotoxicity and anthelmintic activities [2].…”

Section: Introductionmentioning

confidence: 99%

“…Persicaria, a clad in Polygonaceae containing approximately 120 species, grows on the edge of rivers, canals, lakes, and besides the road of tropical and sub-tropical countries of Asia [1]. P hydropiper decoctions are used in the treatment of various ailments including inflammation, dyspepsia, diarrhea, menorrhagia, hemorrhoids, helminthiasis and CNS disorders [2]. The leaves of P. hydropiper is used as carminative, astringent, diuretic and significant CNS depressant activities [3].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of sedative and anxiolytic activities of methanol extract of leaves of Persicaria hydropiper in mice

Shahed-Al-Mahmud

Lina

2017

Clin Phytosci

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Background: Persicaria hydropiper Linn. (Family: Polygonaceae), it grows in damp places and the bank of rivers, canals, lakes, and roadsides of Bangladesh. The leaves of the plant used in traditional medicine to treat insomnia, depression, and neurodegenerative diseases. The objective of this study is to investigate the sedative and anxiolytic activities of the methanol extract of leaves of Persicaria hydropiper (MEPH).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of sedative and anxiolytic activities of methanol extract of leaves of Persicaria hydropiper in mice

Shahed-Al-Mahmud

Lina

2017

Clin Phytosci

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“…[5] As reported by several investigators, the plants consist of primary metabolites, secondary metabolites and micronutrients. [6][7][8][9][10] But the Some of the Rh.Cr was kept for activities and the remaining was suspended in sufficient amount of water followed by fractionation with various solvents in separating funnel. The fractionation was started with less polar n-hexane (500 mL × 3), then chloroform (500 mL × 3), ethyl acetate (500 mL × 3) and final fraction obtained was aqueous.…”

Section: Introductionmentioning

confidence: 99%

“…The fractionation was started with less polar n-hexane (500 mL × 3), then chloroform (500 mL × 3), ethyl acetate (500 mL × 3) and final fraction obtained was aqueous. [9] Similarly, the fractions obtained were n-hexane (Rh. Hex), ethyl acetate (Rh.EtAc), chloroform (Rh.Cf) and aqueous fraction (Rh.Aq).…”

Section: Introductionmentioning

confidence: 99%

Nutritional and medicinal aspects of Rumex hastatus D. Don along with in vitro anti-diabetic activity

Ahmad

Ullah

Ayaz

et al. 2019

International Journal of Food Properties

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Rumex hastatus being used for medicinal and nutritional purposes as a functional food in various countries is hereby evaluated by the gas chromatography-mass spectroscopy, proximate analysis, physicochemical (fluorescence) analysis, quantitative analysis of secondary metabolites and sensory evaluations studies. Various samples of R. hastatus were also evaluated for in vitro anti-diabetic potential. The investigational study demonstrated that R. hastatus is a rich source of carbohydrate, i.e., 432.4 mg/g. Moisture content, protein, fiber, ash content and fats were recorded as 22.8%, 133.9 mg/g, 124.4 mg/g, 54.5 mg/g and 25.6 mg/g, respectively. In the same way, the secondary metabolite displayed a relatively greater amount of flavonoids (84.5 mg/g) followed by saponins (65.5 mg/g) and alkaloids (49.5 mg/g). Similarly, the GC (FID-MS) analysis of R. hastatus revealed the detection of 120 compounds. Out of those identified compounds, selected anti-diabetic compounds were sorted out, viz butyl phthalate, phytol, ethylthreonine, dihydrobenzofuran, indoline, guanidine, nerolidol, myristic acid, palmitic acid, caryophyllene, anozol. In physicochemical fluorescence analysis and the sensory evaluation, data were also recorded along with the anti-diabetic with IC 50 value of 42.09 µg/ml. The overall investigational analysis of R. hastatus obviously demonstrated that this plant was a rich source of primary and secondary metabolites. It may be concluded from the GC (FID-MS) analysis that R. hastatus is a potential source of anti-diabetic constituents, which may confer hypoglycemic potential. Based on the recorded data it may also be inferred that R. hastatus is among safe and nutritious herbs, which can be used in lieu of green vegetables and functional food with anti-diabetic potential. ARTICLE HISTORYdifference is that each plant species differs in the amount and concentration of these substances. The plants rich in primary metabolites are usually used for nutritional purposes, while secondary metabolites are preferred for medicinal purposes. [6,11,12] The plants may possess fats, protein, carbohydrates, vitamins and minerals in such quantity that are considered sufficient for our daily requirements. One can use specific species of plant as food that possess required amount of primary metabolites and low amount of secondary metabolites. But the plants that possess high amount of secondary metabolites than the primary metabolites can cause serious health complications. [13,14] So this is necessary to figure out the amount of nutritional and medicinal elements in those plants, which are being used as food by people.The nutritional values of various plants have been reported by different investigators. [15,16] Diabetes mellitus is a metabolic disorder characterized by increase in blood glucose level. It can affect individuals at any stage of life but the frequency of diabetes is considerably high among the obese and aged people. [17] Various therapeutic measures are employed to alleviate the symptoms of this disease. One of t...

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“…Further, some inhibition of the bovine lens aldose reductase by various sulfated flavonoid of this plant suggests that the plant might have some antidiabetic activity [18,19]. Recently, the phytochemical, phytotoxic and antihelmintic activity of crude methanolic extract of this plant has been investigated [20].…”

Section: Introductionmentioning

confidence: 99%

A preliminary evaluation of cytotoxicity, antihyperglycemic and antinociceptive activity of Polygonum hydropiper L. ethanolic leaf extract

et al. 2016

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Background: Polygonum hydropiper L. is used by folk medicinal practitioners of Bangladesh. Juice of leaves is used in headache, pain, toothache, liver enlargement, gastric ulcer, dysentery, loss of appetite and dysmenorrhea; roots are used as stimulant; juice is applied to wounds, skin. In this study, we analyze the cytotoxicity property of aerial parts of the plant along with antihyperglycemic and antinociceptive activity. Methods: Cytoxicity was determined by brine shrimp lethality assay and antihyperglycemic activity was measured by oral glucose tolerance tests. Antinociceptive activity was determined by observing decreases in abdominal writhings in intraperitoneally administered acetic acid-induced pain model in mice. Results: Administration of ethanol extract of leaf led to dose-dependent and significant reductions in blood glucose levels in glucose-loaded mice. Blood sugar levels of the tested mice were reduced significantly by 48.8, 51.5, 54.1, 58.2 % (p < 0.05) compared to control mice with the increasing dosage of the extract such as 50, 100, 200 and 400 mg/kg of body weight respectively. In contrast, when glibenclamide, a standard antihyperglycemic drug was administered at a dose of 10 mg/kg body weight, it reduced blood glucose level by 42.1 %. On the other hand stem extract only reduced 1.5 % of blood sugar level of the tested mice which was not significant. In the case of antinociceptive activity tests, the extract at the above four doses reduced the number of abdominal writhings by 14.10, 17.95, 29.49 and 41.02 % respectively in comparison with a standard drug Aspirin. In cytotoxicity tests, nauplli were treated with a gradually increased concentration of the extract, ranging from 10 to 45 μg/ml. The LC 50 was found at a concentration of 16.22 μg/ml, compared with control. Conclusion:The results of the present study showed that Polygonum hydropiper L. ethanolic leaf extract possess antihyperglycemic and antinociceptive activity. All these effects could be due to the bioactive components of this plant and need to be identified for further research about this plant. This could justify its ethnomedical use.

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Heavy metals analysis, phytochemical, phytotoxic and anthelmintic investigations of crude methanolic extract, subsequent fractions and crude saponins from Polygonum hydropiper L

Cited by 56 publications

References 28 publications

Evaluation of sedative and anxiolytic activities of methanol extract of leaves of Persicaria hydropiper in mice

Evaluation of sedative and anxiolytic activities of methanol extract of leaves of Persicaria hydropiper in mice

Nutritional and medicinal aspects of Rumex hastatus D. Don along with in vitro anti-diabetic activity

A preliminary evaluation of cytotoxicity, antihyperglycemic and antinociceptive activity of Polygonum hydropiper L. ethanolic leaf extract

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