Helper T cell subpopulations from women are more susceptible to the toxic effect of sodium arsenite in vitro

Differential effect of sodium arsenite during the activation of human CD4+ and CD8+ T lymphocytes

“…In the presence of the highest concentrations of NaAsO 2 tested (4-5 AM), a dramatic reduction of CD4 + and CD8 + cells was observed. A reduction in CD4 + activated T cells in the presence of 1 AM of NaAsO 2 has recently been reported, but in PBMC from women only [42]. We did not analyze differences among gender; we only analyzed PBMC from male donors, since we have observed a variation in the susceptibility to NaAsO 2 in women, related to the menstrual cycle (Tenorio et al, unpublished observations).…”

Leptin Is Oversecreted by Respiratory Syncytial Virus-Infected Bronchial Epithelial Cells and Regulates Th2 and Th17 Cell Differentiation

“…Immunofluorescence was performed as previously described [20] . Briefly, slides were incubated with primary antibodies against ERK1/2 or phospho-ERK1/2 for 1 h and then incubated with FITC-conjugated or PE-conjugated anti-IgG …”

Arsenic and the Cardiovascular System

“…In a similar study, dose-dependent exposure to sodium arsenite (0.001, 0.1, and 1 μM) showed that 1 μM was more toxic to T-helper (CD4 + ) than T-cytotoxic (CD8 + ) cells in human PBMCs. Furthermore, PBMCs from females demonstrated a more reduced T-lymphocyte cell proliferation than males, suggesting that females may be more susceptible to arsenic toxicity [40]. The study by Morzadec et al [41] demonstrated that non-cytotoxic concentrations of NaAs (0.25-2 µM) significantly reduced T cell proliferation by increasing the percentage of non-dividing cells via causing a block in the G1 phase and preventing cyclin D3 and CDC25A expression, which may be IL-2 independent.…”