Hemin‐phytic Acid Functionalized Porous Conducting Polymer Hydrogel With Good Biocompatibility for Electrochemical Detection of H₂O₂ Released From Living Cells

Abstract

A novel hemin/phytic acid doped polyaniline (PA‐PANI) hydrogel composite was prepared through a simple chemical and self‐assembly method, which was modified onto electrode for electrochemical detection of H2O2 released from living cells. It showed good analytical performance with high sensitivity, selectivity and a rapid response for the analysis of H2O2 in the range of 2 to 102 μM, with the detection limit of 1.2 μM. The favorable results mainly originated from both the high conductivity of PA‐PANI hydrogel and its network structure preventing hemin from self‐dimerization to provide active catalytic species. Furthermore, PA‐PANI with good biocompatibility allowed living cells to adhere and resulted in a short diffusion distance between H2O2 released from cells and electrode.

Synergism of Holmium Orthovanadate/Phosphorus-Doped Carbon Nitride Nanocomposite: Nonenzymatic Electrochemical Detection of Hydrogen Peroxide

“…The LOD and the sensitivity of HoVO 4 /P-CN/GCE are calculated as 0.003 μM and 0.72 μA μM –1 cm –2 , respectively. The analytical performance of the proposed HoVO 4 /P-CN-modified GCE for H 2 O 2 detection is compared to the previous reports in Table . ,− This analytical study reveals that the proposed HoVO 4 /P-CN nanocomposite-based electrochemical sensor shows superior or equivalent sensing performance for H 2 O 2 detection compared with the formerly reported electrochemical platforms. The superior electrochemical performance of the HoVO 4 /P-CN nanocomposite is attributed to good electrical conductivity, synergistic effect, large surface area, and more active sites.…”

Recent Progress in Biomedical Sensors Based on Conducting Polymer Hydrogels

“…The ECL sensor exhibited a detection range of 0.01–40 μM with an excellent detection limit of 3.3 nM and also good selectivity in the presence of other interfering molecules. Likewise, a similar hemin/phytic acid-doped PANI hydrogel composite was used to fabricate an electrode for another electrochemical in situ detection of hydrogen peroxide . In this work, instead of using ABEI-Ag, Shang et al functionalized the surface of the hydrogel with hemin, a natural metalloporphyrin that has a peroxidase-like ability (Figure c).…”

Recent theranostic applications of hydrogen peroxide-responsive nanomaterials for multiple diseases

“…In other studies, hydrogels also had great applications in the eld of biosensing. [72][73][74] Guo et al designed L012@PAni-PAAm hydrogels as biosensors to monitor H 2 O 2 produced from cardiomyocytes. 72 In conclusion, organic nanomaterials play a very important role in H 2 O 2 -related biomedical applications.…”

“…In other studies, hydrogels also had great applications in the field of biosensing. 72–74 Guo et al designed L012@PAni-PAAm hydrogels as biosensors to monitor H 2 O 2 produced from cardiomyocytes. 72…”