Hemolysin and K antigens in relation to serotype and hemagglutination type of Escherichia coli isolated from extraintestinal infections

Evans, Doyle J.; Evans, Dolores G.; Höhne, C; Noble, Michael A.; Haldane, E. V.; Lior, H.; Young, Lowell S.

doi:10.1128/jcm.13.1.171-178.1981

Cited by 96 publications

(48 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In particular, strains of MRHA types VI and VII and MSHA type III were over 60% Hly+, in contrast to types V (16%) and IVB (HA-, 8%). This is in general agreement with previous studies (8,13,17) of unselected UTI isolates, i.e., not restricted to the eight most important 0-types.…”

Section: Resultssupporting

confidence: 93%

“…In this hospital, strains of serotypes 01, 02, 04, 06, 07, 09, 018, and 075 cause approximately 7, 2, 5, 10, 3, 2, 5, and 9% of UTI, respectively. Several characteristics of urinary strains are strongly associated with particular 0-types, including hemolysin production (Hly) (predominantly serotypes 06, 075, 018, and 04) (8,13,15) and colicin V production (Cva) (serotypes 09 and 01). It also seems (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Many cell surface components have been shown to be associated with E. coli strains causing extraintestinal infections, including fimbriae (hemagglutination [HA] antigens) (3,8,13,16,30) and specific 0 and K antigens (3,8,14,31), as have the extracellular proteins colicin V (6) and hemolysin (3,5,8,13,17). Although the effects of these factors on virulence characteristics such as adhesion and serum resistance are in certain cases partially elucidated, it remains unclear to what extent they interact to determine virulence in the strains of E. coli isolated from UTI and to what extent they are associated with the establishment of clinical symptoms in the host.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Hemolysin Production as a Virulence Marker in Symptomatic and Asymptomatic Urinary Tract Infections Caused by Escherichia coli

Hughes¹,

Hacker²,

Roberts

et al. 1983

Infect Immun

112

View full text Add to dashboard Cite

Potential virulence, as defined by combined levels of adhesion to urinary epithelial cells, serum resistance, and mouse toxicity, was assessed for Escherichia coli strains causing symptomatic and asymptomatic urinary tract infections in relation to the carriage of hemolysin and other suspected virulence determinants. Hemolysin production (Hly), associated with certain 0 (04, 06, 018, and 075), K (5), and hemagglutination (VI and VII) antigenic types but not colicin V production (Cva), was evident in 83 and 60% of isolates in groups possessing high potential virulence and in only 11 and 6% of those with low virulence. Strains of particular 0-types were not more virulent per se, but among the serotypes, specific combinations of virulence factors appeared decisive, e.g., 018 HAVI B/D/G Hly+ K5'/and 018 HAIII/IVB/V Hly-Cva'/-K1'/strains were, respectively, of high and low potential virulence. Isolates with high potential virulence were found to a similar extent in symptomatic and asymptomatic infections. 546 on July 3, 2020 by guest http://iai.asm.org/ Downloaded from

show abstract

Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Hemolysin Production as a Virulence Marker in Symptomatic and Asymptomatic Urinary Tract Infections Caused by Escherichia coli

Hughes¹,

Hacker²,

Roberts

et al. 1983

Infect Immun

112

View full text Add to dashboard Cite

show abstract

“…Uropathogenic E. coil exhibit a constellation of pathogenic features: they usually belong to a restricted number of O and K antigen serogroups (19,20); they are resistant to the bactericidal action of normal human serum (2t, 22); they secrete hemolysin (23,24) and produce colicin V (25,26); and they attach to uroepithelial cells in vitro (27,28). Epithelial cell adherence is the in vitro analogue of mucosai colonization, an event that appears to precede and be a prerequisite for subsequent pathogenic steps.…”

Section: Discussionmentioning

confidence: 99%

Gal-Gal pyelonephritis Escherichia coli pili linear immunogenic and antigenic epitopes.

Schmidt¹,

O’Hanley²,

Schoolnik³

1985

The Journal of Experimental Medicine

View full text Add to dashboard Cite

The linear immunogenic and antigenic structure of E. coli Gal-Gal pili from the recombinant strain HU 849 was investigated with nine synthetic peptides corresponding to regions of the pilus sequence predicted to contain hydrophilic beta-turns. Five peptides, as bovine serum albumin conjugates, were found by anti-HU 849 pilus serum and were thus designated "immunogenic epitopes." Peptides corresponding to R 25-38, R 38-50, and R 48-61 (which jointly comprise the single intramolecular disulfide loop), and R 103-116, were bound in low titer. A prominent immunogenic epitope was specified by a peptide corresponding to R 65-75. Four peptides, as thyroglobulin conjugates, elicited antisera in rabbits that bound intact HU 849 pili. These were designated "antigenic epitopes." Two prominent antigenic epitopes were localized to peptides corresponding to R 5-12 and R 93-104, whereas peptides corresponding to R 65-75 and R 119-131 represented two minor antigenic epitopes. None of the peptide antisera bound Gal-Gal pili from heterologous strains except anti-R 93-104 and anti-R 5-12. In 8 of the 10 Gal-Gal-binding pyelonephritis isolates tested, anti-R 5-12 detected a protein with an apparent molecular weight of 18,000 co-migrating with several Gal-Gal pili. Anti-R 93-104 detected a corresponding protein in 4 of 8 fecal and 7 of 12 pyelonephritis Gal-Gal-binding isolates; however, it also bound apparently unrelated proteins of higher molecular weight.

show abstract

“…Approximately 50% of Escherichia coli strains causing extra-intestinal infections in humans elaborate a cytolysin, designated E. coli hemolysin (ECH)t, which is responsible for the characteristic zones of (3-hemolysis surrounding bacterial colonies on blood agar (1)(2)(3)(4)(5)(6). Experimental support for a role of this cytolysin as a virulence factor derives from numerous studies with isogenic bacterial strains conducted in animal models (7)(8)(9)(10) .…”

mentioning

confidence: 99%

Potent leukocidal action of Escherichia coli hemolysin mediated by permeabilization of target cell membranes.

Bhakdi

Greulich

Muhly

et al. 1989

The Journal of Experimental Medicine

126

View full text Add to dashboard Cite

The contribution of Escherichia coli hemolysin (ECH) to bacterial virulence has been considered mainly in context with its hemolytic properties. We here report that this prevalent bacterial cytolysin is the most potent leukocidin known to date. Very low concentrations (approximately 1 ng/ml) of ECH evoke membrane permeability defects in PMN (2-10 x 10(6) cells/ml) leading to an efflux of cellular ATP and influx of propidium iodide. The attacked cells do not appear to repair the membrane lesions. Human serum albumin, high density and low density lipoprotein, and IgG together protect erythrocytes and platelets against attack by even high doses (5-25 micrograms/ml) of ECH. In contrast, PMN are still permeabilized by ECH at low doses (50-250 ng/ml) in the presence of these plasma inactivators. Thus, PMN become preferred targets for attack by ECH in human blood and protein-rich body fluids. Kinetic studies demonstrate that membrane permeabilization is a rapid process, ATP-release commencing within seconds after application of toxin to leukocytes. It is estimated that membrane permeabilization ensues upon binding of approximately 300 molecules ECH/PMN. This process is paralleled by granule exocytosis, and by loss of phagocytic killing capacity of the cells. The recognition that ECH directly counteracts a major immune defence mechanism of the human organism through its attack on granulocytes under physiological conditions sheds new light on its possible role and potential importance as a virulence factor of E. coli.

show abstract

Hemolysin and K antigens in relation to serotype and hemagglutination type of Escherichia coli isolated from extraintestinal infections

Cited by 96 publications

References 37 publications

Hemolysin Production as a Virulence Marker in Symptomatic and Asymptomatic Urinary Tract Infections Caused by Escherichia coli

Hemolysin Production as a Virulence Marker in Symptomatic and Asymptomatic Urinary Tract Infections Caused by Escherichia coli

Gal-Gal pyelonephritis Escherichia coli pili linear immunogenic and antigenic epitopes.

Potent leukocidal action of Escherichia coli hemolysin mediated by permeabilization of target cell membranes.

Contact Info

Product

Resources

About