Hemolytic Disease of the Newborn Due to Anti‐Leb

Abstract

. A case of hemolytic disease of the newborn due to anti‐Leb is described. The mother possessed high titer IgG anti‐Leb reacting at 37°C and complement binding in nature. The infant had mild hemolytic disease with a positive direct Coombs' test using anti‐IgG and anticomplement. Anti‐Leb could be eluted from the infant's red cells.

The prozone effect exerted by the complement-binding anti-Lea on anti-D

“…[ 11 ] detected IgG anti-Le a in 12 of the 13 cord sera tested by kinetic enzyme-linked immunosorbent assay indicating that the IgG anti-Le a antibodies do cross the placenta though they do not cause HDNB because of the low levels of Lewis antigens on fetal red cells. However, the mild HDNB due to anti-Le a [ 12 ] and anti-Le b [ 13 14 ] has been documented in literature.…”

Mild Hemolytic Disease of the Newborn due to Anti-Lewis^a

“…HDN due to antibod ies in the Lewis system, although extremely remote, becomes possible. We have found in the literature a single documented case of mild FIDN due to anti-Leb [9], and the case of a newborn with a positive DAT on cord red cells caused by anti-Le", but with no evidence of hemolytic disease [10]. The patient de scribed here seems to be the first reported case, albeit mild, of HDN due to anti-Lea.…”

Detection of IgG Anti-Lewis (a) Antibodies in Cord Sera by Kinetic Elisa

“…Although Lewis blood group antibodies can cause accelerated destruction of antigen positive red blood cel Is in vivo, they rarely, if ever, cause hemolytic disease of the newborn (HDN) [1,2], The usual explanation is that only small amounts of Lewis antigens are on fetal red cells [3] and that Lewis antibodies detectable by hemagglutination are predom inantly IgM and thus do not cross the pla centa [1], However, when sera containing mti-Led are examined by sensitive binding :echniques such as radioimmunoassay [4] rnd kinetic enzyme-linked immunosorbent issay (k-Elisa) [5], IgG Lewis antibodies are Methods trol)]. The same negative control serum was used throughout the study.…”