Hempseed protein derived antioxidative peptides: Purification, identification and protection from hydrogen peroxide-induced apoptosis in PC12 cells

Improvement of ACE inhibitory activity of casein hydrolysate by Maillard reaction with xylose

“…The degree of hydrolysis (DH) was calculated by pH‐stat method . Molecular weight distribution was determined by the method of a previous publication …”

Processing, Nutrition, and Functionality of Hempseed Protein: A Review

“…Depending on the method of extraction employed, the final HPI could vary in protein content, composition, solubility, and, when applied as a functional ingredient, the reactivity with food additives. Alkaline extraction followed by isoelectric precipitation is the most common method for the preparation of HPI (Lu et al., ; Malomo et al., ; Orio et al., ; Raikos, Duthie, & Ranawana, ; Ren et al., ; Tang et al., ; Wang et al., ). The method can produce an isolate with a purity up to 94% depending on the specific extracting conditions, for example, pH, temperature, extraction time, and centrifugal force.…”

“…The specific health‐promoting activities include angiotensin I‐converting enzyme (ACE) inhibition, renin inhibition, acetylcholinesterase (AChE) inhibition, metal‐binding capacity, antioxidant activity, hypocholesterolemic effect, and serum glucose regulation. The bioactivity is elicited through the hydrolysis with gastrointestinal enzymes (pepsin, pancreatin, and trypsin) (Aiello et al., ; Girgih et al., ,b; Girgih, Alashi, He, Malomo, & Aluko, ; Girgih, He, & Aluko, ; Girgih, Udenigwe, & Akuko, , ; Girgih, Udenigwe, Li, Adebiyi, & Aluko, ; Zanoni, Aiello, Arnoldi, & Lammi, ) or exogenous proteases such as alcalase, papain, flavourzyme, neutrase, and thermolysin (Hadnađev et al., ; Lu et al., ; Malomo & Akuko, ; Malomo, Onuh, Girgih, & Aluko, ; Tang, Wang, & Yang, ; Wang, Tang, Chen, & Yang, ). Peptides with ACE inhibitory activity have also been produced from hemp protein with acid hydrolysis (Orio et al., ).…”

“…Lu et al. () also purified two peptides, Asn‐His‐Ala‐Val (NHAV) and His‐Val‐Arg‐Glu‐Thr‐Ala‐Leu‐Val (HVRETALV), from HPH that exhibited high antioxidant activity ( in vitro ) and protective effects against oxidative stress. More recently, two α‐glucosidase inhibitory peptides (Leu‐Arg and Pro‐Leu‐Met‐Leu‐Pro) were isolated from alcalase‐hydrolyzed HPI (Ren et al., ).…”

Sweet potato protein hydrolysates: antioxidant activity and protective effects on oxidative DNA damage

“…The reason for the results above might be due to the increase in basic and hydrophobic amino acids during hydrolysis by alcalase, which were considered to enhance the antioxidant activity of the peptides (Chen et al ., ; Ren et al ., ). In addition, the IC 50 value of alcalase hydrolysates was 1.74 mg mL −1 , which was less than the value (IC 50 7.25 mg mL −1 ) of hempseed protein hydrolysates by alcalase (Lu et al ., ).…”