Heparan sulfates of cultured cells. I. Membrane-associated and cell-sap species in Chinese hamster cells

Kraemer, Paul M.

doi:10.1021/bi00784a026

Cited by 308 publications

(96 citation statements)

References 11 publications

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“…The oxidized products were subsequently cleaved by alkaline elmination at pH 12.0 for 30min at room temperature (Fransson & Carlstedt, 1974). Deaminative cleavage of heparan sulphate was performed by the method of Lagunoff & Warren (1962) (Kraemer, 1971). As shown by Kraemer (1971), no irreversible cell damage occurs under these conditions.…”

Section: Analytical and Separatory Methodsmentioning

confidence: 99%

“…Deaminative cleavage of heparan sulphate was performed by the method of Lagunoff & Warren (1962) (Kraemer, 1971). As shown by Kraemer (1971), no irreversible cell damage occurs under these conditions. Moreover, the cells used in the present study excluded Trypan Blue (>95%Y.)…”

Section: Analytical and Separatory Methodsmentioning

confidence: 99%

“…1977 The cell residue (containing material not solubilized by trypsin) was suspended in water and extracted with trichloroacetic acid (final concn. 7o%, w/v) for 30min in an ice bath (Kraemer, 1971). After centrifugation (10000g for 20min) a precipitate and a supernatant solution were obtained.…”

Section: Analytical and Separatory Methodsmentioning

confidence: 99%

“…Both fractions were then freeze-dried. The trichloroacetic acid extract (soluble material) has been shown to contain cell-sap heparan sulphate (Kraemer, 1971). The medium and the trypsin digest (plus 1 mg of carrier dermatan sulphate) were dialysed against 0.1 M-(NH4)2SO4 for 6h, followed by water for 3 days.…”

Section: Analytical and Separatory Methodsmentioning

confidence: 99%

“…Therefore the significance of a relative enrichment of chondroitin sulphate-like chains in the trypsin digest cannot be evaluated. Although heparan sulphate is considered to be a general cellsurface component (Kraemer, 1971), its biological function remains unknown. Therefore it is difficult to ascertain whether the relatively high content of heparan sulphate in the trypsin digest of lung fibroblasts serves a specific purpose.…”

Section: Structure Of 35s-labelled Galactosaminoglycansmentioning

confidence: 99%

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Synthesis of glycosaminoglycans by human embryonic lung fibroblasts. Different distribution of heparan sulphate, chondroitin sulphate and dermatan sulphate in various fractions of the cell culture

Sjöberg

Fransson

1977

Biochemical Journal

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Foetal human lung fibroblasts, grown in monolayer, were allowed to incorporate 35SO42-for various periods of time. 35S-labelled macromolecular anionic products were isolated from the medium, a trypsin digest of the cells in monolayer and the cell residue. The various radioactive polysaccharides were identified as heparan sulphate and a galactosaminoglycan population (chondroitin sulphate and dermatan sulphate) by ion-exchange chromatography and by differential degradations with HNO2 and chondroitinase ABC. Most of the heparan sulphate was found in the trypsin digest, whereas the galactosaminoglycan components were largely confined to the medium. Electrophoretic studies on the various 35S-labelled galactosaminoglycans suggested the presence of a separate chondroitin sulphate component (i.e. a glucuronic acid-rich galactosaminoglycan). The 35S-labelled galactosaminoglycans were subjected to periodate oxidation of L-iduronic acid residues followed by scission in alkali. A periodate-resistant polymer fraction was obtained, which could be degraded to disaccharides by chondroitinase AC. However, most of the 35S-labelled galactosaminoglycans were extensively degraded by periodate oxidation-alkaline elimination. The oligosaccharides obtained were essentially resistant to chondroitinase AC, indicating that the iduronic acid-rich galactosaminoglycans (i.e. dermatan sulphate) were composed largely of repeating units containing sulphated or non-sulphated L-iduronic acid residues. The L-iduronic acid residues present in dermatan sulphate derived from the medium and the trypsin digest contained twice as much ester sulphate as did material associated with the cells. The content of D-glucuronic acid was low and similar in all three fractions. The relative distribution of glycosaminoglycans among the various fractions obtained from cultured lungfibroblasts was distinctly different from that ofskin fibroblasts [Malmstrom, Carlstedt, Aberg & Fransson (1975) Biochem. J. 151, 477-489]. Moreover, subtle differences in co-polymeric structure of dermatan sulphate isolated from the two cell types could be detected.

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Section: Analytical and Separatory Methodsmentioning

confidence: 99%

Section: Analytical and Separatory Methodsmentioning

confidence: 99%

Section: Analytical and Separatory Methodsmentioning

confidence: 99%

Section: Analytical and Separatory Methodsmentioning

confidence: 99%

Section: Structure Of 35s-labelled Galactosaminoglycansmentioning

confidence: 99%

See 3 more Smart Citations

Synthesis of glycosaminoglycans by human embryonic lung fibroblasts. Different distribution of heparan sulphate, chondroitin sulphate and dermatan sulphate in various fractions of the cell culture

Sjöberg

Fransson

1977

Biochemical Journal

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Changes in the Concentration of Microvilli on the Free Surface of Healing Mesothelium Are Associated With Alterations in Surface Membrane Charge

1996

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Glycosaminoglycans in human lung cancer

Horai

Nakamura

Tateishi

et al. 1981

Cancer

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The quantitative changes of glycosaminoglycans in tumor tissue of human lung cancers (2 squamous cell carcinomas, 4 adenocarcinomas and 5 small cell carcinomas) were studied. The total amount of glycosaminoglycans in human lung cancer tissues increased 1.4 to 4 times in comparison with that in normal lung tissues. The increase in tissue content of glycosaminoglycans was accompanied by an increase in the chondroitin sulfate level in every histologic type of lung cancer, as well as by a marked increase in hyaluronic acid level in squamous cell carcinomas, and a moderate increase in its level in small cell carcinomas. The concentrations of dermatan sulfate and heparan sulfate in lung cancer tissues did not show any significant changes compared with those in normal lung tissues. The increase in total amount and changes in the composition of glycosaminoglycans in human lung cancer tissue were closely related to the histologic type of the tumor.

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Heparan sulfates of cultured cells. I. Membrane-associated and cell-sap species in Chinese hamster cells

Cited by 308 publications

References 11 publications

Synthesis of glycosaminoglycans by human embryonic lung fibroblasts. Different distribution of heparan sulphate, chondroitin sulphate and dermatan sulphate in various fractions of the cell culture

Synthesis of glycosaminoglycans by human embryonic lung fibroblasts. Different distribution of heparan sulphate, chondroitin sulphate and dermatan sulphate in various fractions of the cell culture

Changes in the Concentration of Microvilli on the Free Surface of Healing Mesothelium Are Associated With Alterations in Surface Membrane Charge

Glycosaminoglycans in human lung cancer

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