Hepatitis C virus genotypes and hepatitis G virus in hemodialysis patients from Syria: identification of two novel hepatitis C virus subtypes.

Abdul‐Karim, Ahmad; Zein, Nizar N.; Germer, Jeffrey J.; Kolbert, Christopher P.; Kabbani, L; Krajnik, K L; Hola, Alan; Agha, M N; Tourogman, M; Persing, David H.

doi:10.4269/ajtmh.1998.59.571

Cited by 81 publications

(65 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…HCV genotype 1a was found to be the predominant sub-type among blood donors and Jordanian hemodialysis patients as well as other Middle Eastern countries including Lebanon, Turkey, Cyprus, and Syria [13,14]. HCV genotype 4 is the most prevalent in Saudi Arabia, Egypt, Yemen, and Bahrain [15].…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence and genotyping of hepatitis C virus in multiple transfused Jordanian patients with β-thalassemia major

Al‐Sweedan¹,

Jaradat²,

Amer³

et al. 2011

Turk J Hematol

View full text Add to dashboard Cite

Objective: The main objectives of this study were to investigate the prevalence of hepatitis C virus (HCV) among patients with β-thalassemia major and to determine the most prevalent genotype for this virus. Materials and Methods: One hundred twenty-two β-thalassemia major patients who were previously diagnosed at the molecular level were included. All plasma samples were tested for the presence of antibodies by ELISA. Real-time polymerase chain reaction (PCR) was used in the quantitation the HCV RNA viral loads, and consequently, patients with high virus titer were genotyped by the linear array. Results: Forty of the patients were anti-HCV positive. The prevalence of anti-HCV was significantly higher in patients who received blood transfusion before 1993 (83.7%) than in those who received it after 1993 (16.3%) (p=0.000). β-thalassemia major patients with HCV infection had significantly higher rates of elevated aspartate aminotransferase (54.4% vs 40.5%, p=0.045) and alanine aminotransferase (72.47% vs 37.47%, p=0.00) and of splenectomy (54.8% vs 45.2%, p=0.004) than β-thalassemia major patients without HCV. Conclusion: HCV genotype 4 is the commonest genotype in multi-transfused patients with β-thalassemia major in Jordan. (Turk J Hematol 2011; 28: 47-51) Key words: Virology, transfusion, thalassemia Received: August 7, 2009 Accepted: February 22, 2010 Özet Amaç: Bu çalışma kapsamında, β-talasemi majörlü hastalarda HCV prevalansının araştırılması ve bu virüsün söz konusu hastalar arasında en yaygın genotipinin belirlenmesi amaçlanmaktadır. Yöntem ve Gereçler: Önceden moleküler düzeyde tanı konan 122 β-talasemi majörlü hasta çalışmaya alınmıştır. Tüm plazma numuneleri antikor varlığına yönelik olarak ELISA ile test edilmiştir. HCV RNA

show abstract

Section: Introductionmentioning

confidence: 99%

Seroprevalence and genotyping of hepatitis C virus in multiple transfused Jordanian patients with β-thalassemia major

Al‐Sweedan¹,

Jaradat²,

Amer³

et al. 2011

Turk J Hematol

View full text Add to dashboard Cite

show abstract

“…The distribution of HCV genotypes in liver disease patients living in Syria, Iraq and Iran is unknown. In one study performed in hemodialysis patients from Syria, genotypes 4a, 1b and 1a were identified in respectively 30%, 27% and 19% of patients, and 30% of sequences were unmatched [10]. On the other hand, in countries located on the northern frontiers of Turkey, subtype 1b appears to be the dominant form [11±13].…”

Section: Introductionmentioning

confidence: 99%

Untitled

et al. 2002

View full text Add to dashboard Cite

Abstract. Based on direct sequencing information from 5H UTR and NS5B regions, we identified subtype 1b as a predominant hepatitis C virus genome in Turkey, which affected more than 91% of 79 patients studied. Next, the full genome sequence of a Turkish 1b isolate was obtained by the cloning of polypeptide-encoding region into 7 overlapping fragments. Turkish 1b isolate, which was named HCV-TR1, comprises 9361 nucleotides, including 306 nucleotides of 5 H UTR, a single long open reading frame of 9033 nucleotides, and 22 nucleotides of 3 H UTR. When compared to HCV 1b polypeptide sequences available at GenBank, the predicted polypeptide displayed a total of 36 amino acid substitutions, of which 16 was specific for HCV-TR1 isolate. Despite these changes, major structural and functional motifs of HCV proteins were maintained in HCV-TR1. In contrast, HCV-TR1 displayed amino acid substitutions in 6 out of 9 major cytotoxic T-cell epitopes. These data suggest that HCV-TR1 encodes functionally intact viral proteins, but it also encodes altered viral epitopes, which may affect host immune-response.

show abstract

“…Most infections in Egypt, North Africa, Central Africa, and the Middle East are of genotype 4. Type 5 has been described in South Africa (1,10,11). Genotype 6 isolates are primarily found in Southeast Asia.…”

mentioning

confidence: 99%

Comparison of Hepatitis C Virus Genotyping by 5′ Noncoding Region- and Core-Based Reverse Transcriptase PCR Assay with Sequencing and Use of the Assay for Determining Subtype Distribution in India

et al. 2003

View full text Add to dashboard Cite

Phylogenetic analysis of the sequences of the 5 noncoding regions (5NCR) of 149 samples from hepatitis C virus (HCV) RNA-positive chronic carriers representing northern, southern, eastern, and western India showed that type 3 and type 1 are the predominant genotypes circulating in India, with an overall prevalence of 53.69 and 38.25%, respectively. Type 4 viruses (6.04%) were seen only in southern India. Sequence analysis of the core region of 51 of the above isolates enabled us to classify them further into subtypes as 1b (number of isolates [n] ‫؍‬ 10), 1a (n ‫؍‬ 6), 3a (n ‫؍‬ 9), 3g (n ‫؍‬ 14), 3f (n ‫؍‬ 1), and 4d (n ‫؍‬ 3). Three new subtypes were identified for the first time and designated as 3i (n ‫؍‬ 5), 3j (n ‫؍‬ 2), and 6l (n ‫؍‬ 1). Sequencing the 5NCR could differentiate HCV types, whereas classification at the level of subtype was possible with sequence analysis of the core region.

show abstract

Hepatitis C virus genotypes and hepatitis G virus in hemodialysis patients from Syria: identification of two novel hepatitis C virus subtypes.

Cited by 81 publications

References 35 publications

Seroprevalence and genotyping of hepatitis C virus in multiple transfused Jordanian patients with β-thalassemia major

Seroprevalence and genotyping of hepatitis C virus in multiple transfused Jordanian patients with β-thalassemia major

Untitled

Comparison of Hepatitis C Virus Genotyping by 5′ Noncoding Region- and Core-Based Reverse Transcriptase PCR Assay with Sequencing and Use of the Assay for Determining Subtype Distribution in India

Contact Info

Product

Resources

About