Hepatoprotective activity of Tephrosia purpurea against arsenic induced toxicity in rats

Abstract

Aim:The present study was conducted to evaluate the hepatoprotective activity of Tephrosia purpurea (TP) against sodium arsenite (NaAsO2) induced sub-acute toxicity in rats.Materials and Methods:Twenty four wistar albino rats of either sex were randomly divided into three groups. Group II and III were orally administered with sodium arsenite (10 mg/kg) daily in drinking water for 28 days. Additionally Group III was orally treated with hydro-alcoholic extract of Tephrosia purpurea (TP) @ 500 mg/kg daily for the same time period, whereas only deionized water was given to Group I (control). Serum biomarker levels, oxidative stress parameters and arsenic concentration were assessed in liver. Histopathology was also conducted.Results:It has been seen that TPE (500 mg/kg) significantly (P < 0.01) reduced serum ALT, AST, ALP activity and increased total protein and reduced necrosis and inflammation in liver of group III compared to group II. A significantly (P < 0.01) higher LPO and lower GSH levels without change in SOD activity in liver was also observed in group II compared to group III, though there was no significant difference in arsenic accumulation between them. The plant extract also protects the animals of group III from significant (P < 0.01) reduction in body weight.Conclusion:Our study shows that supplementation of Tephrosia purpurea extract (500 mg/kg) could ameliorate the hepatotoxic action of arsenic.

Anxiolytic and anti-inflammatory role of thymoquinone in arsenic-induced hippocampal toxicity in Wistar rats

“…As neurological implications of arsenic are known, and many chronic and sub-chronic exposures at a much higher dose as this have already been reported ( Gora et al., 2014 ; Kassab and El-Hennamy, 2017 ); we used this exposure regimen of 11 days to mimic sub-acute exposure which might occur accidentally. Dosing regime was planned to have same endpoints and animals were sacrificed on the 12 th day.…”

Effects of olive oil supplementation on sodium arsenate-induced hepatotoxicity in mice

“…Increased levels of serum liver enzymes have been confirmed by previous studies. [ 39 ] It was shown in a study that extra virgin OO decreases oxidative stress and plays a hepatoprotective role. [ 40 ] In this study, the above enzymes increased in OO alone.…”

Arsenic-induced Histological Alterations in Various Organs of Mice

“…The sections of liver in Sa group showed moderate degeneration and necrosis in hepatic parenchyma with mild to moderate fatty change where as control did not reveal any lesions of pathological significance ( Figure 2 ). Earlier, arsenic mediated hepatocytic degeneration was characterized by vacuolar degeneration followed by hepatic necrosis observed in case of rat [ 11 ]. Further, we have observed increased SGPT level in arsenic exposed mice ( Figure 5 ).…”

“…Recently, a series of animal and human epidemiological studies have indicated an association between As exposure and adverse reproductive and developmental outcomes [ 7 – 9 ]. Several line of studies [ 10 , 11 ] have indicated tissue architecture change in heart and hepatic organs by sodium arsenite (Sa). Exposure of Sa showed haemorrhages in myocardium, and degeneration and separation of muscle bundles.…”