Hepatoprotective effect of Bathysa cuspidata in a murine model of severe toxic liver injury

Abstract

The objective of this study was to investigate the hepatoprotective effect of a bark extract of Bathysa cuspidata extract (BCE) in a murine model of severe liver injury induced by carbon tetrachloride (CCl(4) ). Forty-two Wistar rats were randomized into six groups of seven animals each: Group 1(G1): CCl(4) ; Group 2 (G2): dimethyl sulfoxide (DMSO) + CCl(4) ; Group 3 (G3): BCE 400 mg/kg alone; Group 4 (G4): BCE 200 mg/kg + CCl(4) ; Group 5 (G5): BCE 400 mg/kg + CCl(4) ; Group 6 (G6): DMSO alone. The extract was administered by gavage for 18 days beginning 6 days prior to the first application of CCl(4) . After completing CCl(4) administration, the animals were euthanized. The animals in G1, G2, G4 and G5 experienced significant body weight loss and had an increased liver somatic index compared with G3 and G6 (P < 0.05). A significant reduction in serum aspartate and alanine transaminase and gamma-glutamyl transferase (P < 0.05) and a significant increase in the activity of the anti-oxidant enzyme superoxide dismutase were found in G5 (P < 0.05). Lower proportions of cellular necrosis and lipid droplets were found in the livers of animals in G4 and G5 compared with G1 and G2 (P < 0.05). These results confirm the marked hepatoprotective activity of the bark extract of Bathysa cuspidata in severe injuries induced by CCl(4) in rats and suggest that this effect may be associated with the inhibition of oxidative damage.

<b><i>Bathysa cuspidata</i></b> Extract Modulates the Morphological Reorganization of the Scar Tissue and Accelerates Skin Wound Healing in Rats: A Time-Dependent Study

“…According to Novaes et al [2012a], BCE is able to modulate the inflammatory process and promote rapid recovery of lung parenchyma after exposure to toxic agents. Similar results were found by Gonçalves et al [2012a] reporting a decrease in cell death, tissue fibrosis and inflammatory infiltrate in liver tissue after exposure to carbon tetrachloride. Similar to the results of these previous studies, it is not unrealistic to assume a possible relationship between the protective and curative effect of B. cuspidata and with certain phytochemicals found in the BCE, especially polyphenols Novaes et al, 2012a, b].…”

Chemistry and Biological Activity of Condamineeae Tribe: A Chemotaxonomic Contribution of Rubiaceae Family

“…The hepatic injury was induced by intraperitoneal injection with administration of CCl 4 (60% v/v, 1ml/kg) at each 48h during 12 days, the administration of the extract in 200 mg/kg and 400 mg/kg was started six days before the first application of CCl 4 and progressed to be administrated to animals during the 12 days. A significant reduction on serum aspartate transaminase, alanine and gamma-glutamyl transferase and a significant increase on the activity of the antioxidant enzyme superoxide dismutase, low proportions of cellular necrosis and lipid droplets were observed, results that confirm the hepatoprotective activity pronounced of the bark extract of B. cuspidata and suggests this effect can be associated with the inhibition of oxidative damages [28].…”

Long‐lasting morphofunctional remodelling of liver parenchyma and stroma after a single exposure to low and moderate doses of cadmium in rats

“…These findings are consistent with the toxicity mechanisms of many xenobiotics in their ability to induce oxidative stress (Gonçalves et al . ; Novaes et al . ).…”

“…However, depending on the route, dose and exposure time to metal, this balance is disturbed either by increased production of free radicals or by a mechanism inhibiting these enzymes, thereby initiating a process of oxidative stress, which can result in liver injuries (Gonçalves et al . ).…”