<b><i>Bathysa cuspidata</i></b> Extract Modulates the Morphological Reorganization of the Scar Tissue and Accelerates Skin Wound Healing in Rats: A Time-Dependent Study

Abstract

The technological development of pharmaceutical products based on plant extracts is currently responsible for a large number of recent innovations in healthcare. The objective of this study was to develop and investigate the effect and potential applicability of an ointment-based Bathysa cuspidata extract (BCE) for the management of skin wounds in rats. Threeskin wounds of 12 mm in diameter were made on the backs of the animals, which were randomized into 4 groups according to the application received, i.e. the SAL group: 0.9% saline solution, the LAN group: lanolin, the BCE 2.5% group: 2.5% BCE emulsified in lanolin and the BCE 5% group: 5% BCE emulsified in lanolin. The applications were made daily over 21 days, and every 7 days tissue from different wounds was removed. On days 7, 14 and 21, the BCE 2.5% and BCE 5% groups showed the best results in relation to wound closure, and a higher proportion (in length, density and volume) of blood vessels and fibroblasts compared to the other groups. On days 7 and 14, there was a significant increase in the number of mast cells in these 2 groups when compared to the SAL and LAN groups. On day 21, they also had a higher proportion of collagen I than collagen III. B. cuspidata in an ointment base was effective in stimulating tissue cellularity, mast cell recruitment, neoangiogenesis, synthesis and maturation of collagen, epidermal thickness and surface area in scar tissue. These events were potentially related to the best quality and speed for skin regeneration in the rats treated with the BCE ointment.

Augmentation of chronic rotator cuff healing using adipose‐derived stem cell‐seeded human tendon‐derived hydrogel

“…5 During the initial phase of healing after tendon injury, the predominant collagen is type III rather than type I. 36 Therefore, the type I collagen-positive region detected at 2 weeks in this study was most likely derived from injected tHG. Merged images with PKH26 and DAPI revealed that PKH26 positive cells were detected inside and outside of tHG.…”

“…For confirmation of tHG placement after implantation, IHC for type I collagen was performed, since tHG is mainly composed of type I collagen . During the initial phase of healing after tendon injury, the predominant collagen is type III rather than type I . Therefore, the type I collagen‐positive region detected at 2 weeks in this study was most likely derived from injected tHG.…”

Chemistry and Biological Activity of Condamineeae Tribe: A Chemotaxonomic Contribution of Rubiaceae Family

“…After 21 treatment days, the groups with the extract had given better results according to the skin regeneration and wound healing. This study suggests that the healing effects observed in ointment with the B. cuspidata's extract are related to polyphenols compounds, including flavonoids, found in these extracts and which had their presence identified by phytochemical tests [29].…”

Ointment ofBrassica oleraceavar.capitataMatures the Extracellular Matrix in Skin Wounds of Wistar Rats

“…The formulations investigated were prepared as follows: Balsam : B. oleracea var. capitata glycolic extract (10 wt.%), preservatives (methylparaben, propylparaben, and butylated hydroxytoluene [1 : 1, 30 wt.%]), oils (hydrogenated castor oil, triglycerides of caprylic acid, capric acid, caproic acid, and lauric acid [1 : 1, 20 wt.%]), and demineralized water (40 wt.%) [ 18 ]; ointment : B. oleracea var. capitata glycolic extract (10 wt.%), cetostearyl alcohol (5 wt.%), ethoxylated sorbitan monooleate (10 wt.%), Vaseline (10 wt.%), fatty acid with polyol ester (5 wt.%), anhydrous lanolin (15 wt.%), carbamide (5 wt.%), preservatives (methylparaben, propylparaben, and butylated hydroxytoluene [1 : 1, 10 wt.%]), hydrogenated castor oil (5 wt.%), and demineralized water (15 wt.%) [ 18 ]; sunflower oil ( H. annuus ): medium-chain triglyceride oil (caprylic acid, capric acid, caproic acid, and lauric acid [80 wt.%]) and essential fatty acids (linoleic acid [20 wt.%]), in a ratio of 1 : 1 (purchased from Saniplan Essential Products, Rio de Janeiro, RJ, Brazil).…”

“…A grid containing 300 points in a test area of 73 × 10 3 μ m 2 was superimposed on each image for this analysis. The stereological parameters of volume density ( V v ) were calculated by the count of points on fibroblasts, total collagen fibers, and collagen types I and III, using the index V v = P p / P t , in which P p was the number of points occurring over the structure of interest and P t is the total number of points on the test system [ 18 ]. The collagen fibers were analyzed according to their properties of birefringence under polarization.…”