Herbal extracts of Tribulus terrestris and Bergenia ligulata inhibit growth of calcium oxalate monohydrate crystals in vitro

Joshi, V. S.; Parekh, B. B.; Joshi, Mayank; Vaidya, Ashok D.B.

doi:10.1016/j.jcrysgro.2004.11.240

Cited by 70 publications

(54 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our studies are in agreement with the studies previously reported as regards the anti-urolithiatic potency of Tribulus terrestris on the growth COM crystals using double diffusion gel growth technique (21). The anti-urolithiatic ability of the plant is also currently being evaluated in animal models and has exhibited dose-dependent anti-urolithiatic activity and almost completely inhibited stone formation further supporting our results (22,23).…”

Section: Commentssupporting

confidence: 93%

Diminution of oxalate induced renal tubular epithelial cell injury and inhibition of calcium oxalate crystallization in vitro by aqueous extract of Tribulus terrestris

Aggarwal¹,

Tandon²,

Singla

et al. 2010

Int. braz j urol.

View full text Add to dashboard Cite

Purpose: Recurrence and persistent side effects of present day treatment for urolithiasis restrict their use, so an alternate solution, using phytotherapy is being sought. The present study attempted to evaluate the antilithiatic properties of Tribulus terrestris commonly called as "gokhru" which is often used in ayurveda to treat various urinary diseases including urolithiasis. Materials and Methods:The activity of Tribulus terrestris was investigated on nucleation and the growth of the calcium oxalate (CaOx) crystals as well as on oxalate induced cell injury of NRK 52E renal epithelial cells. Results: Tribulus terrestris extract exhibited a concentration dependent inhibition of nucleation and the growth of CaOx crystals. When NRK-52E cells were injured by exposure to oxalate for 72 h, Tribulus terrestris extract prevented the injury in a dose-dependent manner. On treatment with the different concentrations of the plant, the cell viability increased and lactate dehydrogenase release decreased in a concentration dependent manner. Conclusion: The current data suggests that Tribulus terrestris extract not only has a potential to inhibit nucleation and the growth of the CaOx crystals but also has a cytoprotective role. Our results indicate that it could be a potential candidate for phytotherapy against urolithiasis.

show abstract

Section: Commentssupporting

confidence: 93%

Diminution of oxalate induced renal tubular epithelial cell injury and inhibition of calcium oxalate crystallization in vitro by aqueous extract of Tribulus terrestris

Aggarwal¹,

Tandon²,

Singla

et al. 2010

Int. braz j urol.

View full text Add to dashboard Cite

show abstract

“…Thus formed crystals can be easily observed 13 . The glass slide was observed under microscope till it was completely dry.…”

Section: Methods Of Crystal Growthmentioning

confidence: 91%

“…Earlier reported method was used with some modifications 13 . The different stages of calcium oxalate monohydrate crystal growth were studied under a compound microscope.…”

Section: Methods Of Crystal Growthmentioning

confidence: 99%

In Vitro Microscopic Study of Calcium Oxalate Monohydrate Crystals Growth Patterns

Ahmed¹,

Hasan²,

Mahmood³

2016

J. Pharm. Sci. Innov.

View full text Add to dashboard Cite

The purpose of the present study was to explore the possible morphological features and growth patterns of calcium oxalate monohydrate crystals. The study was carried out on a glass slide under microscope. The results showed that the calcium oxalate monohydrate crystals may be present in the form of arborescent, donut, dumbbell, needles, platy, prismatic, rosette, round edges, loose agglomerates and compact aggregates. The study provides detailed information about the morphology and aggregation patterns of calcium oxalate monohydrate crystals.

show abstract

“…The aqueous extract of Costus igneus was prepared with 25g of the shade dried leaves, stems and rhizomes of Costus igneus boiled in 100 ml distilled water for 30 min and then filtered through whatmann filter paper twice [6]. The filtrate was condensed using a rotary evaporator and the residue 1.2 g (leaves), 1.5 g (stems) and 0.8 g (rhizomes) obtained were used to prepare the series (0.15, 0.25, 0.50, 0.75 and 1.0%) of aqueous supernatant concentrations for in vitro studies (table 1).…”

Section: Collection and Extraction Of Plant Materialsmentioning

confidence: 99%

“…Epidemiological data collected during several decades showed that the majority of stones, up to 80%, are composed mainly of calcium oxalate (CaOx) [5]. Calcium-containing stones are the most common comprising about 75% of all urinary calculi, which may be in the form of pure calcium oxalate (50%) or calcium phosphate (5%) and a mixture of both (45%) [6,7]. Calcium oxalate stones are found in two different varieties, calcium oxalate monohydrate or whewellite and calcium oxalate dihydrate or weddellite [8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Growth Characterization of Calcium Hydrogen Phosphate Dihydrate Crystals Influenced by Costus Igneus Aqueous Extract

Yuvarani

Manjula²,

Gopu

2017

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

Objective: To investigate the inhibitory effect of aqueous extract of leaves, stems and rhizome of Costus igneus on the growth of calcium hydrogen phosphate dihydrate (CHPD) crystals. Methods:Calcium hydrogen phosphate dihydrate (CHPD) crystals were grown by the single diffusion gel growth technique and the inhibitory effect of aqueous extracts of leaves, stems and rhizome of Costus igneus on the growth of CHPD crystals has been studied. The grown crystals were characterised by Fourier Transform Infrared Spectroscopy (FTIR), Powder X-Ray diffraction (XRD) for further confirmations. Results:With an increase in the concentration of aqueous extract of Costus igneus, the weight of the formed crystals were gradually reduced from 2.03 g to 0.06 g (leaves), 0.05 g (rhizome), 0.03 g (stem) for the CHPD crystals, respectively. The crystals harvested from the CHPD were characterised by Fourier Transform Infrared Spectroscopy (FTIR) to confirm the functional groups, and Powder X-Ray Diffraction technique (XRD) analyses to confirm the crystalline phases of the CHPD and hydroxyapatite (HAP) crystals. Results obtained indicated that Costus igneus (leaves, stems and rhizome) has the potential to inhibit the formation of calcium hydrogen phosphate dihydrate crystals. Conclusion:This study confirms that using an aqueous extract of stem and rhizome of Costus igneus can promote the formation of hydroxyapatite (HAP) crystals and reduce the nucleation rate of CHPD crystals, a major component of calcium urinary stone.

show abstract

Herbal extracts of Tribulus terrestris and Bergenia ligulata inhibit growth of calcium oxalate monohydrate crystals in vitro

Cited by 70 publications

References 12 publications

Diminution of oxalate induced renal tubular epithelial cell injury and inhibition of calcium oxalate crystallization in vitro by aqueous extract of Tribulus terrestris

Diminution of oxalate induced renal tubular epithelial cell injury and inhibition of calcium oxalate crystallization in vitro by aqueous extract of Tribulus terrestris

In Vitro Microscopic Study of Calcium Oxalate Monohydrate Crystals Growth Patterns

Growth Characterization of Calcium Hydrogen Phosphate Dihydrate Crystals Influenced by Costus Igneus Aqueous Extract

Contact Info

Product

Resources

About