Herstellung heterologer Immunseren gegen Lymphocyten des Hundes

Pichlmayr, R.; Brendel, W.; Beck, G.; Schmittdiel, E.; Tidow, G.; Pichlmayr, Ina

doi:10.1007/bf01716908

Cited by 20 publications

(5 citation statements)

References 16 publications

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“…Das nach vollständiger Gerinnung des entnommenen Blutes gewonnene Serum weist nicht nur eine Antikörperwirkung gegen Lymphozyten, sondern mit unterschiedlicher Stärke auch gegen Erythrozyten, Thrombozyten, Leukozyten, andere Gewebszellen und Serumprotein auf. Und zwar werden agglutinierende, zytotoxische und präzipitierende Antikörper festgestellt (12,36). Bei unseren Immunseren wurden zum Beispiel die in Tabelle 2 angegebenen Antikörpertiter gegen andere Zellen als Lymphozyten gefunden.…”

Section: Die Absorption Toxischer Antikörperunclassified

“…Dies gilt insbesondere für die jüngsten Versuche von Starzl (41), der, wie aus seinen Angaben deutlich hervorgeht, zu wenig mit Erythrozyten absorbiert hat. Man muß immerhin bedenken, daß zur vollständigen Absorption einer bestimmten Serummenge mitunter die zehnfache Menge von Blut benötigt wird (36). Nach Absorption der antierythrozytären Antikörper waren die gewonnenen Seren jedoch in allen bisher bekannten Versuchen an Hunden und Kaninchen gut verträglich und führten abgesehen von schwachen anaphylaktischen Reaktionen innerhalb der ersten vier Wochen zu keiner wesentlichen Beeinträchtigung des Testtieres.…”

Section: Die Absorption Toxischer Antikörperunclassified

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Heterologe Antilymphozytenseren zur Immunsuppression bei Organtransplantationen

Brendel¹,

Pichlmayr²

1967

Dtsch med Wochenschr

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Section: Die Absorption Toxischer Antikörperunclassified

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Heterologe Antilymphozytenseren zur Immunsuppression bei Organtransplantationen

Brendel¹,

Pichlmayr²

1967

Dtsch med Wochenschr

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“…In view of the present finding that, in the rabbit at least, multiple booster injections lead to a progressive decline in the potency of ALS, it is of interest to note that several of the preparations of ALS currently in clinical use have been harvested from horses that have received multiple booster injections of human lymphoid tissue (8,9). While it is true that ALS of demonstrable immunosuppressive activity has been raised in horses which received multiple booster injections of dog lymphocytes (10,11), it would appear desirable to investigate the possibility that at some point increasing immunization of the horse, like the rabbit, may yield ALS of diminished potency.…”

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Effective and Ineffective Antilymphocyte Sera Correlation of in Vitro Tests with Potency of Early Antisera

Davis

Cooperband

Mannick

1970

Experimental Biology and Medicine

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“…The effect of the antilymphoid sera depended on their activity in a lymphocyte monoculture.Heterologous antisera against lymphocytes (ALS) or thymocytes (ATS) of a recipient give rise to a marked immunodepressive effect during allografting [1,2,[7][8][9][10]12]. A convenient working model for studying the mechanism of action of antilymphoid sera on lymphoid ceils is a culture of circulating blood lymphocytes.…”

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Investigation of activity of antilymphoid sera in cultures of human circulating blood lymphocytes

Govallo¹,

Grigor'eva²,

Kopelyan³

et al. 1970

Bull Exp Biol Med

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Individual heterologous antilymphoid sera act differently on the blast-transformation reaction in mixed cultures of homologous human circulating blood lymphocytes. The effect of the antilymphoid sera depended on their activity in a lymphocyte monoculture.Heterologous antisera against lymphocytes (ALS) or thymocytes (ATS) of a recipient give rise to a marked immunodepressive effect during allografting [1,2,[7][8][9][10]12]. A convenient working model for studying the mechanism of action of antilymphoid sera on lymphoid ceils is a culture of circulating blood lymphocytes. In the presence of ALS, mitotic activity of lymphocytes in culture is stimulated [3,5,6,11]. However, there is no information in the literature concerning the effect of ALS on a mixed culture of lymphocytes.The object of this investigation was to study the action of various heterologous antisera against human lymphoid cells on monocultures and mixed cultures of circulating blood lymphocytes. EXPERIMENTAL Antisera were obtained by immunizing rabbits and horses with a suspension of human fetal lymphocytes or thymocytes~ The scheme of immunization and method of obtaining antisera were described by the writers previously. Altogether 8 antisera were obtained for testing: 4 rabbit ATS, 2 rabbit ALS, and 2 horse ALS. The antibody titer in the antisera was tested by the lymphocyte-agglutination reaction (LAR) and the lymphocytetoxic test (LCT).The blast-transformation reaction in monocultures and mixed cultures of lymphocytes (BTR) was carried out by the writers ~ modification of the method described in [4]. Monocultures and mixed cultures of homologous lymphocytes obtained from 23 apparently healthy persons were studied~ The activity of the antilymphoid sere was estimated by their ability to cause blast transforming, cytotoxic and leukocyte-agglutinating effects in vitro. The cytotoxic effect was assessed quantitatively from the relative number of lysed and degenerated ceils (under 10% +, 10-20%++, 30-40~c +++, 50-60~c++++, and 60% +++++). The effect of the antisera on the BTR in a mixed culture was assessed from the change in intensity of reaction of ceils preliminarily tested with ALS or ATS.In all series of experiments the following standard scheme of treatment of lymphocytes was used. Whole antiserum, in a dose of 0.1 ml per million lymphocytes, was added to the monoculture~ Treatment of the lymphocyte partners during cultivation was carried out in the presence of a dose of 0~ ml per million cells. After incubation for 30 rain at 37 ~ the cells were washed with medium No. 199 and mixed in the proportion of 1 : 1 for subsequent cultivation by the usual scheme.

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Herstellung heterologer Immunseren gegen Lymphocyten des Hundes

Cited by 20 publications

References 16 publications

Heterologe Antilymphozytenseren zur Immunsuppression bei Organtransplantationen

Heterologe Antilymphozytenseren zur Immunsuppression bei Organtransplantationen

Effective and Ineffective Antilymphocyte Sera Correlation of in Vitro Tests with Potency of Early Antisera

Investigation of activity of antilymphoid sera in cultures of human circulating blood lymphocytes

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