Herstellung rekombinanter Proteine mit <i>Pichia pastoris</i> in integrierter Prozessführung

Cornelissen, Gesine; Bertelsen, H.‐P.; Hahn, B.; Schultz, M. A.; Scheffler, Uwe; Werner, E.; Leptien, H.; Krüß, S.; Jansen, Ann-Katrin; Gliem, T.; Hielscher, M.; Wilhelm, Bettina; Sowa, Eveline; Radeke, Heinfried H.; Luttmann, R.

doi:10.1002/cite.200390058

Cited by 4 publications

(1 citation statement)

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“…Advantages of integrating the separation process into the production would avoid inhibition by product feedback, prevent accumulation of toxic substances, minimize product decomposition and simplify downstream processing. There are some reports where the integration of cell separation into the production process has already been performed, for instance in the production of recombinant proteins using the yeast Pichia pastoris (Cornelissen et al [26]) or of organic acids with lactobacilli (Pai et al, Richter and Nottelmann [27,28]). There is an integrated bioprocess described for the production of biosurfactants, namely the production of rhamnolipids with Pseudomonas aeruginosa (Gruber et al, Ochsner et al [29,30]).…”

Section: Introductionmentioning

confidence: 98%

Sophorolipids from Candida bombicola: Cell separation by ultrasonic particle manipulation

Palme

Comanescu

Stoineva

et al. 2010

Euro J Lipid Sci & Tech

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The yeast Candida bombicola ATCC 22214 is well-known to produce mixtures of glycolipids containing the sugar sophorose, the so-called sophorolipids, especially when cultivated on hydrophobic carbon sources as co-substrates. To improve cultivation efficiency, an integrated process was developed using ultrasound separation technology. Since this technology is new for use with C. bombicola, it was first characterized in batch experiments and afterwards implemented in an integrated production process. In this process, separation efficiencies of about 99% C. bombicola cells could be achieved, leading to 8 g/L of nearly cell-free sophorolipid product and a total amount of 73.8 g/L sophorolipids. Furthermore, a technical mixture of unusual branched fatty alcohols containing mainly 2-hexyl-1-decanol was used as co-substrate with glucose in a shake flask study. This resulted in the production of a new product, 1-O-b-glucopyranosyl-2-hexyldecanol, a molecule containing glucose as the sugar moiety and 2-hexyl-1-decanol as a branched hydrophobic side chain.

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