Hesperidin, a citrus bioflavonoid, decreases the oxidative stress produced by carbon tetrachloride in rat liver and kidney

Abstract

BackgroundCCl4 is a well-established hepatotoxin inducing liver injury by producing free radicals. Exposure to CCl4 also induces acute and chronic renal injuries. The present study was designed to establish the protective effect of hesperidin (HDN), a citrus bioflavonoid, on CCl4-induced oxidative stress and resultant dysfunction of rat liver and kidney.MethodsAnimals were pretreated with HDN (100 and 200 mg/kg orally) for one week and then challenged with CCl4 (2 ml/kg/s.c.) in olive oil. Rats were sacrificed by carotid bleeding under ether anesthesia. Liver enzymes, urea and creatinine were estimated in serum. Oxidative stress in liver and kidney tissue was estimated using Thiobarbituric acid reactive substances (TBARS), glutathione (GSH) content, superoxide dismutase(SOD), and Catalase (CAT)ResultsCCl4 caused a marked rise in serum levels of ALT and AST (P < 0.05). TBARS levels were significantly increased whereas GSH, SOD and CAT levels decreased in the liver and kidney homogenates of CCl4 treated rats. HDN (200 mg/kg) successfully attenuated these effects of CCl4ConclusionIn conclusion, our study demonstrated a protective effect of HDN in CCl4 induced oxidative stress in rat liver and kidney. This protective effect of HDN can be correlated to its direct antioxidant effect.

Ameliorative Action of Mn-Salen Derivatives on CCl&lt;sub&gt;4&lt;/sub&gt;-Induced Destructive Effects and Lipofuscin-Like Pigment Formation in Rats’ Liver and Brain: Post-Treatment of Young Rats with EUKs

“…According to the literature data, radicals formed through metabolic conversion of CCl 4 , initiate the peroxidation of membrane poly-unsaturated fatty acids (PUFA) [11] [30] [33] with ultimate production of MDA and 4-HNE [3] [33]. These aldehydes are believed to be the main indicator of age pigment formation.…”

“…Several studies have indicated that CCl 4 produces free radicals with consequent harmful damage to tissues such as the liver, kidneys, heart, lung and brain as well as blood [11]. One mechanism behind these damaging effects is the formation of trichloromethyl ( 3 CCl  ) and trichloromethylperoxyl ( 3 2 CCl O  ) radicals and the covalent binding of 3 CCl  to vital target molecules such as DNA, lipids, proteins and carbohydrates.…”

“…CCl 4 is a lipid-soluble toxic agent used commonly to induce oxidative stress in rats [11]. Several studies have indicated that CCl 4 produces free radicals with consequent harmful damage to tissues such as the liver, kidneys, heart, lung and brain as well as blood [11].…”

“…The accumulation of these pigments can be the inevitable consequence of normal oxygen metabolism, which is associated with a surplus formation of reactive oxygen species (ROS) [7]. In addition, the range of exogenous factors such as different kinds of radiation, exposure to heavy metals, drugs and xenobiotics and carbon tetrachloride (CCl 4 ) may induce the lipofuscin and LFPs formation [8]- [11].…”

Assessment of Hepatoprotective Role of Phenolic Extract of Urtica Dioica and Silver Nanoparticles in Male Rat Induced by Carbon Tetra-Chloride

“…The belief causes of carbon tetrachloride are provoked hepatic destruction in lipid peroxidation and decline action of antioxidant enzymes and accumulation of free radicals. 6,7 Urtica dioica L. herbaceous plant that grows over a wide area of the world belongs to the family Urticaceae and includes more than sixty genera and about more than seven hundred species the commonly known as Stinging Nettle. 8,9 The diskette leaves rich in fine hair, which irritates the skin on contact because they contain many materials including acetylcholine, histamine.…”