Heteroassembled gold nanoparticles with sandwich-immunoassay LSPR chip format for rapid and sensitive detection of hepatitis B virus surface antigen (HBsAg)

Kim, Jinwoon; Oh, Sejong; Shukla̽, Shruti; Hong, Seongkweon; Heo, Nam Su; Bajpai, Vivek K.; Chun, Hyang Sook; Jo, Cheon-Ho; Choi, Bong Gill; Huh, Yun Suk; Han, Young‐Kyu

doi:10.1016/j.bios.2018.02.019

Cited by 103 publications

(66 citation statements)

References 23 publications

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“…For example, Thamm utilized this system for sensing DNA in real time by immobilizing gold nanoparticles on glass . In other study, this particles have been used in exosome detection, cancer therapy, hepatitis B virus detection …”

Section: Resultsmentioning

confidence: 99%

“…39 In other study, this particles have been used in exosome detection, 40 cancer therapy, 41 hepatitis B virus detection. 42 These properties in one hand are benefiting since make gold particles useful in bio sensing and drug delivery on the other hand are hazard since they can attach to variety of biomolecules, enzymes and DNA, which can cause abnormalities. They showed no or minimal acute toxicity of gold nanoparticles in zooplankters behavior.…”

Section: Fluorescence Response To Aumentioning

confidence: 99%

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A novel optical probe based on d‐penicillamine‐functionalized graphene quantum dots: Preparation and application as signal amplification element to minoring of ions in human biofluid

Pashazadeh-Panahi

Hasanzadeh

Eivazzadeh‐Keihan

2020

J of Molecular Recognition

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In this study, d‐penicillamine‐functionalized graphene quantum dots (DPA‐GQD) has been synthesized, which significantly increases the fluorescence intensity of GQD. We used this simple fluorescent probe for metal ions detection in human plasma samples. Designed DPA‐GQD respond to Hg2+, Cu2+, Au2+, Ag+, Co2+, Zn2+, and Pb2+ with high sensitivity. The fluorescence intensity of this probe decreased significantly in the presence of metal ions such as, Hg2+, Cu2+, Au2+, Ag+, Co2+, Zn2+, and Pb2+. In this work, a promising probe for ions monitoring was introduced. Moreover, DPA‐GQD probe has been tested in plasma samples. The functionalized DPA‐GQDs exhibits great promise as an alternative to previous fluorescent probes for bio‐labeling, sensing, and other biomedical applications in aqueous solution.

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Section: Resultsmentioning

confidence: 99%

Section: Fluorescence Response To Aumentioning

confidence: 99%

A novel optical probe based on d‐penicillamine‐functionalized graphene quantum dots: Preparation and application as signal amplification element to minoring of ions in human biofluid

Pashazadeh-Panahi

Hasanzadeh

Eivazzadeh‐Keihan

2020

J of Molecular Recognition

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“…Moreover, inhibited NETs do not effectively trap and kill the virus, which may be another reason for HBV persistence. HBsAg is the first indicator and an important biomarker for the diagnosis of HBV infection(42).…”

Section: Discussionmentioning

confidence: 99%

Hepatitis B Virus Inhibits Neutrophil Extracellular Traps Release by Modulating Reactive Oxygen Species Production and Autophagy

Liu

Gao

et al. 2018

Preprint

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19Neutrophils, an important component of the innate immune system, release 20 extracellular traps (NETs) to eliminate invaded pathogens by trapping and killing 21 microbes. A dysfunctional innate immune response is a major cause of persistent 22 hepatitis B virus (HBV) infection. HBV has been shown to reduce neutrophil 23 responses. The objectives of the present study were to determine whether HBV 24 influenced NETs release and to identify the underlying mechanisms. Primary 25 neutrophils and circulating blood samples were collected from 40 patients with a 26 chronic hepatitis B infection (CHB) and 40 healthy controls to detect NETs release 27 using a Quant-iT Pico Green dsDNA assay and to determine the levels of HBV-DNA 28 and HBV markers. NETs release was decreased in patients with a CHB infection, and 29 hepatitis B surface antigen, hepatitis B e antigen and hepatitis B core antibody levels 30 negatively correlated with NETs release. The Quant-iT Pico Green dsDNA assay and 31 western blotting were used to examine the effect of HBV proteins (HBV X protein, 32 HBV C protein, HBV E protein and HBV S protein) on NETs release in vitro. Based 33 on the flow cytometry and western blot data, HBV C protein and HBV E protein34 inhibited NETs release by decreasing reactive oxygen species (ROS) production and 35 autophagy. Overall, HBV may inhibit NETs release by modulating ROS production 36 and autophagy to escape the immune system and promote the establishment of a 37 chronic infection. 38 39 40 41 Hepatitis B virus (HBV) infection is a serious liver disease. Owing to its chronicity 42 and the increased risk of hepatocellular carcinoma, it has become a global burden. 43 HBV has infected 2 billion people, 360 million chronically, and leads to 44 approximately 600,000 deaths each year(1). A dysfunctional innate immune response 45 is a major cause of persistent HBV infection(2). Neutrophils [polymorphonuclear 46 neutrophil granulocytes (PMNs)] are effector cells involved in innate antimicrobial 47 defence and are also related to liver disease, since have key functions in the 48 pathogenesis of alcoholic hepatitis(3). Neutrophils employ three major strategies to 49 combat microbes: phagocytosis, degranulation, and the release of neutrophil 50 extracellular traps (NETs), a process referred to as NETsosis(4), which differs from 51 apoptosis and necrosis(5). NETs are extracellular fibrous structures composed of 52 chromatin, histones, and several proteins, such as neutrophil elastase (NE), 53 myeloperoxidase (MPO), cathepsin G, and proteinase 3 (PR3)(6-9). These large 54 extracellular structures trap and kill a variety of microbes by exposing them to high 55 concentrations of NETs-associated microbicidal factors and providing a physical 56 barrier to prevent microbial dissemination(6, 10). In addition to their roles in infection, 57 NETs were recently shown to have roles in various sterile diseases, such 58 autoinflammatory and autoimmune diseases(11). 59 The intracellular signalling pathways that regulate NETs formation are s...

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“…Moreover, AuNPs do not disturb the functional activity even after antigen immobilization (Choi and Frangioni 2010;Sonawane and Nimse 2016). The antibody-antigen reaction is enhanced by the surface functionalization of gold nanoparticles, thereby increasing immunoassay signals, which ultimately increase the test sensitivity (Kim et al 2018). It offers an easy, low-cost assay, which allows simultaneously numerous sample testing.…”

Section: Gold Nanoparticle (Aunps)-based Diagnostics For Tbmentioning

confidence: 99%

Diagnosis of Tuberculosis: Nanodiagnostics Approaches

Gupta

Singh

2020

NanoBioMedicine

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Tuberculosis (TB) remains one of the most devastating infectious diseases worldwide. The burden of TB is alarmingly high in developing countries, where diagnosis latent TB infection (LTBI), Extra-pulmonary tuberculosis (EPTB), drug-resistant tuberculosis (DR-TB), HIV-associated TB, and paediatric TB is still a challenge. This is mainly due to delayed or misdiagnosis of TB, which continues to fuel its worldwide epidemic. The ideal diagnostic test is still unavailable, and conventional methods remain a necessity for TB diagnosis, though with poor diagnostic ability. The nanoparticles have shown potential for the improvement of drug delivery, reducing treatment frequency and diagnosis of various diseases. The engineering of antigens/antibody nanocarriers represents an exciting front in the field of diagnostics, potentially flagging the way toward development of better diagnostics for TB. This chapter discusses the presently available tests for TB diagnostics and also highlights the recent advancement in the nanotechnology-based detection tests for M. tuberculosis.

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Heteroassembled gold nanoparticles with sandwich-immunoassay LSPR chip format for rapid and sensitive detection of hepatitis B virus surface antigen (HBsAg)

Cited by 103 publications

References 23 publications

A novel optical probe based on d‐penicillamine‐functionalized graphene quantum dots: Preparation and application as signal amplification element to minoring of ions in human biofluid

A novel optical probe based on d‐penicillamine‐functionalized graphene quantum dots: Preparation and application as signal amplification element to minoring of ions in human biofluid

Hepatitis B Virus Inhibits Neutrophil Extracellular Traps Release by Modulating Reactive Oxygen Species Production and Autophagy

Diagnosis of Tuberculosis: Nanodiagnostics Approaches

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