Heterogeneity of changes in lymphoproliferative ability with increasing age

Murasko, Donna M.; Nelson, Barbara J.; Matour, Deborah; Goonewardene, I. Michael; Kaye, Donald

doi:10.1016/0531-5565(91)90020-m

Cited by 34 publications

(19 citation statements)

References 11 publications

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“…We saw a wide range of values in the immune parameters and inflammatory markers in these elderly patients demonstrating diversity or heterogeneity. This is consistent with other investigators who have observed this same phenomenon (wide variability) when studying T-suppressor activity in patients with heart failure [9], Variation in measures of cellular immune function in the elderly is widely reported, and our study is consistent with this observation [30][31][32], It is therefore particularly interesting that in this study we observed a significant correlation between TNF levels and NK activity, such that varia tion in TNF levels accounted for about half the variation in NK activity, and differences in Z)-dimer levels accounted for an additional component of the variation.…”

Section: Discussionsupporting

confidence: 82%

Tumor Necrosis Factor, Natural Killer Activity and Other Measures of Immune Function and Inflammation in Elderly Men with Heart Failure

Pritchett¹,

Cohen²,

Rao³

et al. 1995

Gerontology

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Objective: To determine the status of tumor necrosis factor (TNF) and other measures of immunity and inflammation in chronic heart failure (CHF) in the elderly. Design: Comparative survey study of subjects with heart failure and age-matched controls. Settings: University affiliated tertiary care VA Medical Center, Heart Failure Clinic. Patients: Twenty men with New York Class II and III heart failure and 17 age-matched controls. Interventions: None. Main outcome measure: Levels of lymphocyte mitogenesis, TNF, natural killer (NK) cell activity, elastase-Α₁-antitrypsin (E/Α) and cross-linked fibrin D-dimers (XDP). Results: TNF levels (p = 0.27), NK activity (p = 0.56), and lymphocyte mitogenesis (p = 0.67) were similar in patients and controls. E/Α levels were somewhat lower in CHF patients (p = 0.05) and XDP were similar (p = 0.59). However, TNF levels were significantly related to NK activity and to E/Α activity in elderly men with heart failure but not controls. XDP were positively related to NK in heart failure patients but not controls. Conclusion: TNF and other measures of immune function and inflammation do not appear to be significantly elevated in elderly patients with heart failure of moderate severity. However, significant relationships exist between TNF, NK activity, XDP and E/Α in the heart failure patients only, suggesting that immune activation and subclinical inflammation does exist in these patients.

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Section: Discussionsupporting

confidence: 82%

Tumor Necrosis Factor, Natural Killer Activity and Other Measures of Immune Function and Inflammation in Elderly Men with Heart Failure

Pritchett¹,

Cohen²,

Rao³

et al. 1995

Gerontology

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“…Differences in sample size appear to distinguish studies that found an age-related difference in IL-2 production from those that did not. All but one of the studies that found a difference evaluated 10-17 elderly [10,14,18,[20][21][22], while 3 of the 4 studies reporting comparable IL-2 production between young and elderly subjects evaluated 31-46 elderly [9,23,Bernstein et al submitted]. Interestingly, one of the larger studies that demonstrated no age-related difference in IL-2 production by mitogen-induced PBMC also evaluated IL-2 production by PBMC induced with anti-CD2/CD28 antibodies [23].…”

Section: Il-2mentioning

confidence: 95%

“…For example, while peak lymphocyte proliferation or cytokine production of young and elderly subjects occurs on the same day after stimulation, the young response peaks and falls sharply, while the elderly response peaks and slowly declines (i.e. flattened curve) [10]. Since most studies measure proliferation and cytokine production at one time point and one concentration of stimulus, differences among studies may reflect subtle shifts in these conditions.…”

Section: Variation In Induction and Measurement Of Cytokinesmentioning

confidence: 98%

“…It is critical to understand that even within subpopulations of the elderly classified by health status, there is considerable heterogeneity of immune response [9,10]. In any given sampling of elderly subjects there will be a certain percentage with immune responses similar to young controls, a percentage that respond at a very low level, with the majority responding at 50-75% of the level of young controls [10]. Whether the heterogeneity reflects health status, genetic variability, or behaviors such as smoking or level of physical activity, is not fully understood.…”

Section: Health Status Of Subjectsmentioning

confidence: 99%

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Effect of age on cytokine production in humans

Bernstein

Murasko

1998

AGE

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Aging is accompanied by many changes in immune response, with the most consistent and dramatic alterations occurring within the T cell compartment. Since cytokines are central to immune cell communications, age-associated changes in cytokine production may contribute to these alterations. While data from murine studies suggest a switch from a Th1 (IL-2, IFNγ) to a Th2 (IL-4, IL-6, IL-10) cytokine response, this model has not been as clearly established in humans. In addition, this current review of over 50 studies in humans suggests that age-associated changes in cytokine production are not consistent.

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“…We also analyze the cell type specificity and conservation of related genomic sequences of EPC-1 as a measure of both the generality of its expression and the potential tendency of its loss in an age-associated manner. Murasko et al (1991). All other cell lines used in this study were obtained from the Aging Cell Repository (Coriell Institute, Camden, NJ).…”

mentioning

confidence: 99%

Analysis of EPC‐1 growth state–dependent expression, specificity, and conservation of related sequences

Pignolo

Rotenberg

Cristofalo

1995

Journal Cellular Physiology

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The transcript for EPC-1 (early population doubling level (PDL) cDNA-1) is induced under conditions of growth arrest due to density-dependent contact inhibition and/or serum deprivation in early-passage but not in senescent WI-38 fibroblasts. We have characterized the EPC-1 transcript with respect to its cell-cycle regulation, tissue specificity, and interspecies conservation of related genomic sequences. In low density, quiescent (serum-deprived), early-passage fibroblasts that are stimulated to proliferate with fresh serum, steady-state EPC-1 transcript levels are steadily reduced until they reach a basal level approximately 24 h after stimulation. However, when early-passage fibroblasts are made quiescent by both serum deprivation and density-dependent contact inhibition and then stimulated with serum, steady-state EPC-1 transcript levels remain relatively constant throughout a 36 h period following serum stimulation. Senescent WI-38 cells (> 95% life span completed) do not express EPC-1 under these conditions. We show that differences in the regulation of EPC-1 transcript levels in early-passage cells are due to differences in growth state rather than changes in cell density or contact. We also show that expression of the EPC-1 transcript is limited to specific cell types and that related genomic sequences are found in all mammalian species examined as well as in the chicken.

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Heterogeneity of changes in lymphoproliferative ability with increasing age

Cited by 34 publications

References 11 publications

Tumor Necrosis Factor, Natural Killer Activity and Other Measures of Immune Function and Inflammation in Elderly Men with Heart Failure

Tumor Necrosis Factor, Natural Killer Activity and Other Measures of Immune Function and Inflammation in Elderly Men with Heart Failure

Effect of age on cytokine production in humans

Analysis of EPC‐1 growth state–dependent expression, specificity, and conservation of related sequences

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