Heterogeneity of Genotypic and Phenotypic Characteristics of Fifteen Permanent Cell Lines Derived from Human Gliomas

Bigner, Darell D.; Bigner, Sandra H.; Pontén, Jan; Westermark, Bengt; Mahaley, M. S.; Ruoslahti, Erkki; Herschman, Harvey; Eng, Lawrence F.; Wikstrand, Carol J.

doi:10.1097/00005072-198105000-00001

Cited by 432 publications

(185 citation statements)

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“…For example, Rettig et al (1986) found that only six of 22 malignant glioma lines were GFAP positive. Similar results have been reported by other investigators (Bigner et al, 1981). Transfection of a GFAP-de®cient malignant glioma line with a functional GFAP cDNA decreased cell proliferation and growth in soft agar (Rutka and Smith, 1993), suggesting a role for GFAP in maintaining a reduced transformed state.…”

Section: Introductionsupporting

confidence: 88%

“…Both the rpL32 and actin probes indicate that similar amounts of RNA were loaded in each lane with the possible exception of M059J which had low levels of both these control RNAs (Figure 4c and d). U138MG has previously been reported to be negative for GFAP mRNA and protein (Bigner et al, 1981;Tohyama et al, 1993). The U138MG line that we tested was obtained from Dr Fogh (Sloane Kettering Institute, NY) in 1973 and may represent an earlier version of the cell line tested by others or may represent a selected subpopulation which is positive for GFAP.…”

Section: Expression Of B-fabp and Gfap Mrna In Malignant Gliomamentioning

confidence: 96%

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Correlation of B-FABP and GFAP expression in malignant glioma

et al. 1998

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The murine brain fatty acid binding protein (B-FABP) is encoded by a developmentally regulated gene that is expressed in radial glial cells and immature astrocytes. We have cloned the human B-FABP gene and have mapped it to chromosome 6q22-23. We show that B-FABP mRNA is expressed in human malignant glioma tumor biopsies and in a subset of malignant glioma cell lines, as well as in human fetal retina and brain. Malignant glioma tumors are characterized by cytoplasmic bundles of glial ®brillary acidic protein (GFAP), a protein normally expressed in mature astrocytes. Establishment of malignant glioma cell lines often results in loss of GFAP. The subset of malignant glioma cell lines that express GFAP mRNA also express B-FABP mRNA. Co-localization experiments in cell lines indicate that the same cells produce both GFAP and B-FABP. We suggest that some malignant gliomas may be derived from astrocytic precursor cells which can express proteins that are normally produced at di erent developmental stages in the astrocytic di erentiation pathway.

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Section: Introductionsupporting

confidence: 88%

Section: Expression Of B-fabp and Gfap Mrna In Malignant Gliomamentioning

confidence: 96%

Correlation of B-FABP and GFAP expression in malignant glioma

et al. 1998

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“…Both cell lines were isolated from human glioblastomas and various characteristics have been described (Bigner et al, 1981;Bongcam-Rudloff et al, 1991;Uhrbom et al, 1997;Hussaini et al, 1999). U-1242-Z cells are U-1242 cells that stably express PKC-Z (Hussaini et al, 2000).…”

Section: Cell Culturesmentioning

confidence: 99%

The protein kinase C-η isoform induces proliferation in glioblastoma cell lines through an ERK/Elk-1 pathway

et al. 2006

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“…The human D-54Mg cell line was kindly supplied by Dr DD Bigner, Duke University Medical Center, Durham, NC, USA (Bigner et al, 1981). The GaMg was obtained from a 42-year-old woman and histologically identified as a glioblastoma (Akslen et al, 1988).…”

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confidence: 99%

Proliferation, migration and invasion of human glioma cells exposed to paclitaxel (Taxol) in vitro

Terzis

Thorsen²,

Heese³

et al. 1997

Br J Cancer

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Summary Paclitaxel (Taxol), an anti-cancer drug derived from Taxus species, was tested for its anti-migrational, anti-invasive and antiproliferative effect on two human glioma cell lines (GaMg and D-54Mg) grown as multicellular tumour spheroids. In addition, the direct effect of paclitaxel on glioma cells was studied using flow cytometry and scanning confocal microscopy. Both cell lines showed a dose-dependent growth and migratory response to paclitaxel. The GaMg cells were found to be 5-10 times more sensitive to paclitaxel than D-54Mg cells. Paclitaxel also proved to be remarkably effective in preventing invasion in a co-culture system in which tumour spheroids were confronted with fetal rat brain cell aggregates. Control experiments with Cremophor EL (the solvent of paclitaxel for clinical use) in this study showed no effect on tumour cell migration, cell proliferation or cell invasion. Scanning confocal microscopy of both cell lines showed an extensive random organization of the microtubules in the cytoplasm. After paclitaxel exposure, the GaMg and the D-54Mg cells exhibited a fragmentation of the nuclear material, indicating a possible induction of apoptosis. In line with this, flow cytometric DNA histograms showed an accumulation of cells in the G/M phase of the cell cycle after 24 h of paclitaxel exposure. After 48 h, a deterioration of the DNA histograms was observed indicating nuclear fragmentation.

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Heterogeneity of Genotypic and Phenotypic Characteristics of Fifteen Permanent Cell Lines Derived from Human Gliomas

Cited by 432 publications

References 0 publications

Correlation of B-FABP and GFAP expression in malignant glioma

Correlation of B-FABP and GFAP expression in malignant glioma

The protein kinase C-η isoform induces proliferation in glioblastoma cell lines through an ERK/Elk-1 pathway

Proliferation, migration and invasion of human glioma cells exposed to paclitaxel (Taxol) in vitro

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