Heterogeneity of Human Urinay Glycosaminoglycans

Reducing terminals and molecular weights of glycosaminoglycans in normal human urine.

“…Although the presence of mammalian endoglucuronidases for heparin (Ogren and Lindahl 1975) and for heparan sulfate (Klein and Figura 1976a, b;Klein et al 1976;Kindler et al 1977) has been reported, endoglycuronidase for chondroitin sulfate isomers was not found yet. Heparin was not detected in any subfraction (Endo et al 1980a), but heparan sulfate was contained in some of the subfractions examined in the present study, which might be degraded by the action of the above endoglucuroni dase for heparan sulfate. However, 0.75 M Fr-1.5 M Fr of 60% Fr, 1.25 M Fr of 50% Fr and 1.5 M Fr of 30% Fr-50 % Fr did not contain heparan sulfate, but contained chondroitin sulfates and dermatan sulfate.…”

“…The acidic subfractions examined in the present study were the specimens of 20 % Fr-60% Fr obtained in Report I in this series (Endo et al 1980a). …”

“…To elucidate the reducing terminals and the molecular weights of urinary GAG, the reducing power of the GAG in the acidic subfractions obtained in Report I (Endo et al 1980a) was determined.…”

Polydispersity of glycosaminoglycans in normal human urine.

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To elucidate precise nature of urinary glycosaminoglycans (GAG), GAG in the acidic subfractions obtained in a previous paper (Endo et al 1980) were quantitated by digestion with mucopolysaccharidases, nitrous acid treatment and by quantitative cellulose acetate membrane (Separax) electrophoresis.Hyaluronic acid, chondroitin sulfates and chondroitin, dermatan sulfate and dermatan, heparan sulfate, keratan sulfate, and acidic glycopeptide were found in wide distribution in these subfrac tions. The results suggested the presence of various degree of the depolymerization products of each GAG and of wide range of the desulfation products of each sulfated GAG.

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“…To elucidate precise nature of urinary glycosaminoglycans (GAG), GAG in the acidic subfractions obtained in a previous paper (Endo et al 1980) were quantitated by digestion with mucopolysaccharidases, nitrous acid treatment and by quantitative cellulose acetate membrane (Separax) electrophoresis.…”