Heterogeneous Post‐Translational Modification of <i>Actinobacillus actinomycetemcomitans</i> Fimbrillin

Inoue, Tetsuyoshi; Ohta, Hiroyuki; Tanimoto, Ichiro; Shingaki, Ryuji; Fukui, Kiichi

doi:10.1111/j.1348-0421.2000.tb02554.x

Cited by 22 publications

(27 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The involvement of glycoconjugates in the adherence of A. actinomycetemcomitans to surfaces was also suggested by Fine et al (3). With regard to this, we previously proposed the possibility that the fimbriae of A. actinomycetemcomitans are glycosylated (8). However, the present result suggests that surface-exposed carbohydrate residues other than fimbriae-associated residues are involved in the biofilm formation.…”

Section: Disorganization Of 310-df Biofilm With Sodium Metaperiodate contrasting

confidence: 55%

See 1 more Smart Citation

Biofilm Formation by a Fimbriae‐Deficient Mutant of Actinobacillus actinomycetemcomitans

Inoue

Shingaki

Sogawa

et al. 2003

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

Actinobacillus actinomycetemcomitans strain 310‐TR produces fimbriae and forms a tight biofilm in broth cultures, without turbid growth. The fimbriae‐deficient mutant 310‐DF, constructed in this study, was grown as a relatively fragile biofilm at the bottom of a culture vessel. Scanning electron microscopy revealed that on glass coverslips, 310‐TR formed tight and spherical microcolonies, while 310‐DF produced looser ones. These findings suggest that fimbriae are not essential for the surface‐adherent growth but are required for enhancing cell‐to‐surface and cell‐to‐cell interactions to stabilize the biofilm. Treatment of the 310‐DF biofilm with either sodium metaperiodate or DNase resulted in significant desorption of cells from glass surfaces, indicating that both carbohydrate residues and DNA molecules present on the cell surface are also involved in the biofilm formation.

show abstract

Section: Disorganization Of 310-df Biofilm With Sodium Metaperiodate contrasting

confidence: 55%

“…The fimbriae are composed of subunits of approximately 6.5 kDa (7), which are post-translationally modified (8), and share amino acid sequence similarity with type IV pilin of gram-negative bacteria (7,12). Recent genetic and molecular biological studies have revealed that the flp-rcp-tad gene cluster is required for fimbriae production (5,7,11).…”

mentioning

confidence: 99%

Biofilm Formation by a Fimbriae‐Deficient Mutant of Actinobacillus actinomycetemcomitans

Inoue

Shingaki

Sogawa

et al. 2003

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…This pilus is encoded by a cluster of 14 chromosomal genes (13), the first of which, flp-1, encodes the major pilin subunit (14). A. actinomycetemcomitans Flp-1 protein been shown to be posttranslationally modified, probably by glycosylation (11). It is possible that this posttranslational glycosylation includes an N-acetylglucosamine component and that cleavage of this sugar by DspB alters the adhesive properties of the pili, thereby releasing cells from the aggregate.…”

Section: Discussionmentioning

confidence: 99%

Detachment of Actinobacillus actinomycetemcomitans Biofilm Cells by an Endogenous β-Hexosaminidase Activity

et al. 2003

View full text Add to dashboard Cite

When cultured in broth, fresh clinical isolates of the gram-negative periodontal pathogen Actinobacillus actinomycetemcomitans form tenaciously adherent biofilm colonies on surfaces such as plastic and glass. These biofilm colonies release adherent cells into the medium, and the released cells can attach to the surface of the culture vessel and form new colonies, enabling the biofilm to spread. We mutagenized A. actinomycetemcomitans clinical strain CU1000 with transposon IS903kan and isolated a transposon insertion mutant that formed biofilm colonies which were tightly adherent to surfaces but which lacked the ability to release cells into the medium and disperse. The transposon insertion in the mutant strain mapped to a gene, designated dspB, that was predicted to encode a secreted protein homologous to the catalytic domain of the family 20 glycosyl hydrolases. A plasmid carrying a wild-type dspB gene restored the ability of biofilm colonies of the mutant strain to disperse. We expressed A. actinomycetemcomitans DspB protein engineered to contain a hexahistidine metal-binding site at its C terminus in Escherichia coli and purified the protein by using Ni affinity chromatography. Substrate specificity studies performed with monosaccharides labeled with 4-nitrophenyl groups showed that DspB hydrolyzed the 134 glycosidic bond of ␤-substituted N-acetylglucosamine, which is consistent with the known functions of other family 20 glycosyl hydrolases. When added to culture medium, purified DspB protein, but not heat-inactivated DspB, restored the ability of the mutant strain to release cells and disperse. DspB protein also caused the detachment of cells from preformed biofilm colonies of strain CU1000 grown attached to plastic and the disaggregation of highly autoaggregated clumps of CU1000 cells in solution. We concluded that dspB encodes a soluble ␤-N-acetylglucosaminidase that causes detachment and dispersion of A. actinomycetemcomitans biofilm cells.

show abstract

“…The glycan was proven to be crucial for collagen binding and the protection of EmaA against proteolytic degradation (439). Previously, the potential glycosylation of the Flp fimbriae was reported (440).…”

Section: Protein Glycosylationmentioning

confidence: 99%

The Sweet Tooth of Bacteria: Common Themes in Bacterial Glycoconjugates

Tytgat

Lebeer

2014

Microbiol Mol Biol Rev

129

View full text Add to dashboard Cite

SUMMARY Humans have been increasingly recognized as being superorganisms, living in close contact with a microbiota on all their mucosal surfaces. However, most studies on the human microbiota have focused on gaining comprehensive insights into the composition of the microbiota under different health conditions (e.g., enterotypes), while there is also a need for detailed knowledge of the different molecules that mediate interactions with the host. Glycoconjugates are an interesting class of molecules for detailed studies, as they form a strain-specific barcode on the surface of bacteria, mediating specific interactions with the host. Strikingly, most glycoconjugates are synthesized by similar biosynthesis mechanisms. Bacteria can produce their major glycoconjugates by using a sequential or an en bloc mechanism, with both mechanistic options coexisting in many species for different macromolecules. In this review, these common themes are conceptualized and illustrated for all major classes of known bacterial glycoconjugates, with a special focus on the rather recently emergent field of glycosylated proteins. We describe the biosynthesis and importance of glycoconjugates in both pathogenic and beneficial bacteria and in both Gram-positive and -negative organisms. The focus lies on microorganisms important for human physiology. In addition, the potential for a better knowledge of bacterial glycoconjugates in the emerging field of glycoengineering and other perspectives is discussed.

show abstract

Heterogeneous Post‐Translational Modification of Actinobacillus actinomycetemcomitans Fimbrillin

Cited by 22 publications

References 14 publications

Biofilm Formation by a Fimbriae‐Deficient Mutant of Actinobacillus actinomycetemcomitans

Biofilm Formation by a Fimbriae‐Deficient Mutant of Actinobacillus actinomycetemcomitans

Detachment of Actinobacillus actinomycetemcomitans Biofilm Cells by an Endogenous β-Hexosaminidase Activity

The Sweet Tooth of Bacteria: Common Themes in Bacterial Glycoconjugates

Contact Info

Product

Resources

About