Heterologous expression of fungal AcGDH alleviates ammonium toxicity and suppresses photorespiration, thereby improving drought tolerance in rice

Lü, Yan; Gong, Yinyin; Luo, Qiong; Dai, Gaoxing; Teng, Zhonghua; He, Yong; Wu, Xiangxia; Liu, Cong; Tang, Dongying; Ye, Nenghui; Deng, Gaofeng; Lin, Juan; Liu, Xuanming

doi:10.1016/j.plantsci.2020.110769

Cited by 12 publications

(7 citation statements)

References 54 publications

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“…+ toxicity [24]. Interestingly, cytokinin could specifically enhance the activity of GDH3 in the roots of N-fed plants, which further indicates that some NAGs might have functional crosstalk on N metabolism and hormone signaling in different organs [25].…”

Section: Introductionmentioning

confidence: 92%

Nitrogen Assimilation Related Genes in Brassicanapus: Systematic Characterization and Expression Analysis Identified Hub Genes in Multiple Nutrient Stress Responses

Zhang

et al. 2021

Plants

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Nitrogen (N) is an essential macronutrient for plants. However, little is known about the molecular regulation of N assimilation in Brassica napus, one of the most important oil crops worldwide. Here, we carried out a comprehensive genome-wide analysis of the N assimilation related genes (NAGs) in B. napus. A total of 67 NAGs were identified encoding major enzymes involved in N assimilation, including asparagine synthetase (AS), glutamate dehydrogenase (GDH), glutamine oxoglutarate aminotransferase (GOGAT), glutamine synthetase (GS), nitrite reductase (NiR), nitrate reductase (NR). The syntenic analysis revealed that segmental duplication and whole-genome duplication were the main expansion pattern during gene evolution. Each NAG family showed different degrees of differentiation in characterization, gene structure, conserved motifs and cis-elements. Furthermore, diverse responses of NAG to multiple nutrient stresses were observed. Among them, more NAGs were regulated by N deficiency and ammonium toxicity than by phosphorus and potassium deprivations. Moreover, 12 hub genes responding to N starvation were identified, which may play vital roles in N utilization. Taken together, our results provide a basis for further functional research of NAGs in rapeseed N assimilation and also put forward new points in their responses to contrasting nutrient stresses.

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Section: Introductionmentioning

confidence: 92%

Nitrogen Assimilation Related Genes in Brassicanapus: Systematic Characterization and Expression Analysis Identified Hub Genes in Multiple Nutrient Stress Responses

Zhang

et al. 2021

Plants

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“…GSH, a major endogenous antioxidant, participates in H 2 O 2 detoxification via various glutathione peroxidases (Gu and Chauhan, 2015). Meanwhile, the GSH synthesis regulated by GDH helps plants absorb the excessive

{NH}_{4}^{+}

to produce glutamate, for the first committed step in GSH synthesis (Skopelitis et al ., 2006; Tercé‐Laforgue et al ., 2013; Yan et al ., 2021). However, the direct role of GDH in PM resistance in apple is unclear.…”

Section: Introductionmentioning

confidence: 99%

The m⁶A reader MhYTP2 regulates MdMLO19 mRNA stability and antioxidant genes translation efficiency conferring powdery mildew resistance in apple

Guo

Liu

Meng

et al. 2021

Plant Biotechnology Journal

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Summary N6‐methyladenosine (m6A) reader protein plays an important role in trichome morphology, developmental timing and morphogenesis in Arabidopsis. However, the function of m6A readers in plant‐microbe interaction remains unclear. Here, a Malus YTH‐domain family protein MhYTP2 was initially characterized as an m6A reader. MhYTP2 overexpression increased mRNA m6A modification level and translation efficiency. The m6A in the exon regions appeared to destabilize the mRNAs, whereas m6A in the untranslated regions positively correlated with the associated mRNA abundance. MhYTP2 overexpression enhanced apple powdery mildew resistance, possibly by rapidly degrading the bound mRNAs of MdMLO19 and MdMLO19‐X1 and improving the translation efficiency of the antioxidant genes. To conclude, the results shed light on the apple m6A profile, the effect of MhYTP2 on m6A profile, and the m6A roles in MdMLO19 and MdMLO19‐X1 mRNAs stability and glutamate dehydrogenase 1‐like MdGDH1L mRNA translation efficiency.

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“…As previously reported in wheat (Vita et al 2018), GDH genes show a higher expression in plants from organic plots compared to those grown using mineral fertilizers. It has also been recently demonstrated that GDH alleviates ammonium toxicity and suppresses photorespiration by assimilating excess NH 4 + and disturbing the delicate balance of carbon and nitrogen metabolism, thereby improving drought tolerance in rice (Yan et al 2021).…”

Section: Resultsmentioning

confidence: 99%

Amino acid metabolism and expression of genes involved in nitrogen assimilation in common oranges cv. Valencia Late

Fabroni¹,

Amenta²,

Rapisarda³

et al. 2022

Biol. plant.

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Biochemical and transcriptional approaches can provide crucial evidence about the physiological changes which can occur in organic and conventional cultivated common orange [Citrus sinensis (L.) Osbeck]. This study aimed to investigate the change in physicochemical parameters, the concentrations of free amino acids and other N-containing compounds, and the expressions of key genes coding for enzymes linked to N assimilation in fruits of common orange cv. "Valencia Late". Two enzymes involved in different ways in N assimilation were considered: nitrate reductase (NR), catalyzing the conversion of nitrate into nitrite, and glutamate dehydrogenase (GDH), operating in the assimilation of ammonium (interacting with glutamate synthase), and in ammonium re-assimilation through glutamate deamination.Results showed that the different fertilizers did not affect the physicochemical characteristics of fruits but induced the different accumulation of free amino acids, with higher concentrations of proline and contemporarily lower concentrations of glutamate, in addition to upregulated the expression of GDH gene in fruits from organically managed tress. This study identified a possible adaptive response of common orange plants to organic or conventional fertilizers. The present work is intended as a first step to make the mechanisms underlying plant responses to N supply clearer by comparing organic and conventional cultivation. It also can support breeders to select the best citrus cultivars and agronomists to improve crop fertilization and production management.

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Heterologous expression of fungal AcGDH alleviates ammonium toxicity and suppresses photorespiration, thereby improving drought tolerance in rice

Cited by 12 publications

References 54 publications

Nitrogen Assimilation Related Genes in Brassicanapus: Systematic Characterization and Expression Analysis Identified Hub Genes in Multiple Nutrient Stress Responses

Nitrogen Assimilation Related Genes in Brassicanapus: Systematic Characterization and Expression Analysis Identified Hub Genes in Multiple Nutrient Stress Responses

The m⁶A reader MhYTP2 regulates MdMLO19 mRNA stability and antioxidant genes translation efficiency conferring powdery mildew resistance in apple

Amino acid metabolism and expression of genes involved in nitrogen assimilation in common oranges cv. Valencia Late

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Heterologous expression of fungal AcGDH alleviates ammonium toxicity and suppresses photorespiration, thereby improving drought tolerance in rice

Cited by 12 publications

References 54 publications

Nitrogen Assimilation Related Genes in Brassicanapus: Systematic Characterization and Expression Analysis Identified Hub Genes in Multiple Nutrient Stress Responses

Nitrogen Assimilation Related Genes in Brassicanapus: Systematic Characterization and Expression Analysis Identified Hub Genes in Multiple Nutrient Stress Responses

The m6A reader MhYTP2 regulates MdMLO19 mRNA stability and antioxidant genes translation efficiency conferring powdery mildew resistance in apple

Amino acid metabolism and expression of genes involved in nitrogen assimilation in common oranges cv. Valencia Late

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The m⁶A reader MhYTP2 regulates MdMLO19 mRNA stability and antioxidant genes translation efficiency conferring powdery mildew resistance in apple