Hexadecane mineralization activity in hydrocarbon-contaminated soils of Ross Sea region Antarctica may require nutrients and inoculation

Aislabie, Jackie; Ryburn, J.; Gutierrez-Zamora, Maria-Luisa; Rhodes, Phillipa; Hunter, David W.F.; Sarmah, Ajit K.; Barker, G. M.; Farrell, Roberta L.

doi:10.1016/j.soilbio.2011.10.001

Cited by 17 publications

(10 citation statements)

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“…Several studies have stressed the importance of using multiple REs (see Thies 2007), either by simultaneous digestion (Bastias et al 2007;Kluber et al 2011;Aislabie et al 2012;Godin et al 2012) or by combining the data (Klamer and Hedlund 2004;Kasel et al 2008;Bennett et al 2009;Trabelsi et al 2012) to obtain an accurate representation of the microbial community composition (Marsh et al 2000). In the present study, the effect of soil treatments on bacterial community composition and the relationship between community composition and environmental variables were lower when the four REs were combined, and when only AluI and MspI were combined, most of the soil treatment replicates did not group together and soil treatment effect was low (data not shown).…”

Section: Different Restriction Enzymes Reveal Different Storiesmentioning

confidence: 99%

Analysing the effect of soil organic matter on bacterial communities using T-RFLP fingerprinting: different methods, different stories?

et al. 2015

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Soil microbial ecology needs robust tools to elucidate ecological questions, such as the impact of fertilisation on soil microbial communities. However, the methods and data analysis used can directly affect the biological conclusions. In this study, the sensitivity of terminal-restriction fragment length polyphorism (T-RFLP) to four restriction enzymes (RE), six peak area thresholds (PAT) from 0 to 10 % and two matrices (presence/absence and relative abundance) was assessed on soils subjected to eight different long-term amendments. The T-RFLP profiles were analysed using a three-step multivariate analysis approach: (i) cluster analysis and non-metric multi-dimensional scaling, (ii) ANOSIM and PERMANOVA and (iii) correlations. The application of organic and mineral fertilisers over 53 years changed the bacterial community composition regardless if the RE, PAT and matrix were used. However, the clustering of the community, the strength of these differences, the correlations with environmental variables and, subsequently, the biological conclusions varied with the use of RE, PAT and matrix. Hence, the bacterial community composition was found to be either highly sensitive to any changes in soil organic matter strongly correlated to C and N concentration, or only affected by large inputs of C or soil management. Different REs can reveal different bacterial populations affected by different drivers, but PATs 0.5 and 1 % should be used especially when using presence/absence matrix. This study also shows the complexity of the effect of organic and mineral amendment on bacterial community composition and stresses the importance to inform on methodological and data analysis parameters.

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Section: Different Restriction Enzymes Reveal Different Storiesmentioning

confidence: 99%

Analysing the effect of soil organic matter on bacterial communities using T-RFLP fingerprinting: different methods, different stories?

et al. 2015

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“…Despite the presence of hydrocarbon-degrading bacteria, petroleum hydrocarbons accumulate for long periods in contaminated Antarctic soils (Cury et al, 2014;de Jesus, Peixoto, Cury, et al, 2015;Martínez Álvarez et al, 2017;Vázquez et al, 2017). As shown by Atlas and Hazen (2011) and Aislabie et al (2012), micro-organisms require elements other than carbon for growth and the availability of N, P, and K can limit rates of oil biodegradation in polar environments. It is therefore essential to gain a better understanding of the complex interactions between soil physical-chemical proprieties and microbial community distribution and metabolism in order to optimize in situ bioremediation of oil-contaminated Antarctic soils.…”

Section: Introductionmentioning

confidence: 99%

Metagenomic analysis of microbial communities across a transect from low to highly hydrocarbon‐contaminated soils in King George Island, Maritime Antarctica

et al. 2021

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Soil samples from a transect from low to highly hydrocarbon-contaminated soils were collected around the Brazilian Antarctic Station Comandante Ferraz (EACF), located at King George Island, Antarctica. Quantitative PCR (qPCR) analysis of bacterial 16S rRNA genes, 16S rRNA gene (iTag), and shotgun metagenomic sequencing were used to characterize microbial community structure and the potential for petroleum degradation by indigenous microbes. Hydrocarbon contamination did not affect bacterial abundance in EACF soils (bacterial 16S rRNA gene qPCR). However, analysis of 16S rRNA gene sequences revealed a successive change in the microbial community along the pollution gradient. Microbial richness and diversity decreased with the increase of hydrocarbon concentration in EACF soils. The abundance of Cytophaga, Methyloversatilis, Polaromonas, and Williamsia was positively correlated (pvalue = <.05) with the concentration of total petroleum hydrocarbons (TPH) and/or polycyclic aromatic hydrocarbons (PAH). Annotation of metagenomic data revealed that the most abundant hydrocarbon degradation pathway in EACF soils was related to alkyl derivative-PAH degradation (mainly methylnaphthalenes) via the CYP450 enzyme family. The abundance of genes related to nitrogen fixation increased in EACF soils as the concentration of hydrocarbons increased. The results obtained here are valuable for the future of bioremediation of petroleum hydrocarbon-contaminated soils in polar environments.

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“…Antarctic mineral soils are generally low in carbon and nutrients and therefore, hydrocarbon spills can increase soil carbon concentrations but can further deplete nitrogen and phosphorus when they are assimilated during biodegradation. Hence, soil C:N ratios of >70 have been measured in contaminated sites, whereas those in control sites were <17 (Aislabie et al 2012).…”

Section: Heavy Metalsmentioning

confidence: 99%

Human Impacts on Soils

O’Neill

Aislabie

Balks

2015

World Soils Book Series

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Hexadecane mineralization activity in hydrocarbon-contaminated soils of Ross Sea region Antarctica may require nutrients and inoculation

Cited by 17 publications

References 50 publications

Analysing the effect of soil organic matter on bacterial communities using T-RFLP fingerprinting: different methods, different stories?

Analysing the effect of soil organic matter on bacterial communities using T-RFLP fingerprinting: different methods, different stories?

Metagenomic analysis of microbial communities across a transect from low to highly hydrocarbon‐contaminated soils in King George Island, Maritime Antarctica

Human Impacts on Soils

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