Hexose transport across the apical and basolateral membrane of enterocytes from different regions of the chicken intestine

Abstract

The properties of hexose transport across the apical and basolateral membranes of chicken enterocytes have been studied in the small and large intestine. Results show that (a) isolated epithelial cells from all segments except the coprodeum can accumulate 3-O-methylglucose (Glc3Me) against a concentration gradient, by a Na(+)-dependent and phloridizin-sensitive mechanism. (b) The cell cumulative capacity for Glc3Me (control/phloridizin-incubated cells) is lower in the small intestine than in the large intestine (rectum = proximal caecum = ileum > jejunum > duodenum). (c) Theophylline enhances the cell Glc3Me cumulative capacity 2.9-fold in the duodenum and 2.4-fold in the jejunum but has no effect in the other segments studied. (d) Analysis of sugar uptake indicates that net hexose influx rates decrease from proximal to distal regions: jejunum > duodenum > ileum = proximal caecum = rectum for the apical transport system (alpha-methyl glucoside as substrate and phloridizin as inhibitor) and duodenum > jejunum > ileum = proximal caecum = rectum for the basolateral system (2-deoxyglucose; theophylline). (e) The duodenum and the jejunum show high apical and basolateral hexose transport rates, which confer a significant capacity for sugar absorption on the proximal intestine. More distal regions, including the ileum, the proximal caecum and the rectum, have transport systems analogous to those of the proximal intestine that keep a considerable potential capability to recover hexoses from the lumen.

Cell Apical Surface Area in Enterocytes from Chicken Small and Large Intestine During Development

“…From a regional point of view, the small intestine showed a considerable homogeneity in MAF at the day of hatch but in 6-wk-old chickens the duodenum and jejunum increased their surface area whereas the ileum remained unchanged. Isolated enterocytes from proximal segments (duodenum, jejunum) of 4-to 7-wk-old chickens, were shown to be better suited for apical hexose uptake than the ileum (Ferrer et al, 1994). Both observations suggested a morphological and functional correlation, which had to be considered to understand the physiology of the digestive system.…”

Influence of deoxynivalenol on the D‐glucose transport across the isolated epithelium of different intestinal segments of laying hens

“…The Na + ‐coupled glucose uptake across SGLT1 is present in all regions of the small and large intestine of chicken and the capacity to transport glucose decreased from the proximal to distal regions of the small intestine (Obst and Diamond, 1989; Ferrer et al., 1994). In our previous study (Awad et al., 2005a), it has been shown that DON inhibited Na + ‐ d ‐glucose co‐transport in the proximal jejunum of laying hens.…”

Effects of resalination on intestinal glucose transport in chickens adapted to low Na+ intakes

“…All regions of the lower intestine also contribute to the resorption of ureteral electrolyte and fluid (Skadhauge, 1993). Furthermore, the proximal caecum and the rectum can also absorb Na¤ cotransported with glucose and amino acids (Lind et al 1980;Moret o & Planas, 1989;Ferrer et al 1994b;Amat et al 1999). All these functions are affected by the NaCl status of the animal since restriction of Na¤ intake results in adaptive regulation, especially in the lower intestine, where there is a marked increase in the apical epithelial Na¤ channel (ENaC; Goldstein et al 1997) and the Na¤-H¤ exchanger (NHE2; Donowitz et al 1998) activities, and in the short-circuit current (Thomas & Skadhauge, 1982;Amat et al 1988).…”