Hibiscus sabdariffa L. water extract inhibits the adipocyte differentiation through the PI3-K and MAPK pathway

Kim, Jin‐Kyung; So, Hong-Seob; Youn, Myung-Ja; Kim, Hyungjin; Kim, Yunha; Park, Channy; Kim, Se-Jin; Ha, Young-Ae; Chai, Kyu Yun; Kim, Shin-Moo; Kim, Ki‐Young; Park, Raekil

doi:10.1016/j.jep.2007.08.028

Cited by 73 publications

(56 citation statements)

References 36 publications

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“…Effect of ADE extract to inhibit the differentiation of 3T3L1 preadipocyte to mature adipocyte was carried out based on reported protocol (Kim et al 2007). Briefly, 3T3L1 preadipocyte cells were maintained by 10 % FBS in DMEM containing 4.5 g/L glucose, 100 U/mL penicillin, 0.1 mg/mL streptomycin and 0.25 mg/mL amphotericin B at 37°C in 5 % CO 2 incubator.…”

Section: Adipocyte Differentiation Assaymentioning

confidence: 99%

Effect on oxidative stress, glucose uptake level and lipid droplet content by Apigenin 7, 4'-dimethyl ether isolated from Piper longum L

2014

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Piper longum L. (Family: Piperaceae), is a widely used herb in several Ayurvedic formulations prescribed for various diseases. Potential of the plant material as an antidiabetic and cardio protective agent has not been evaluated so far. In the study, we designed experiments to evaluate antioxidant, glucose uptake potential and lipid content regulating potential of extracts and compound from P. longum fruits. Solvent extracts from Piper longum fruits using hexane, ethyl acetate, methanol, 70 % methanol-water were taken and apigenin 7, 4'-dimethyl ether (ADE) was isolated from ethyl acetate extract. Antioxidant activity, glucose uptake potential and adipocyte differentiation assay was performed with extract and pure compound. Antioxidant activity in terms of TRP (196.03 μg/mg GAE), DPPH assay (IC 50 -173.09 μg/mL), hydroxyl radical scavenging assay (IC 50 -20.42 μg/mL), inhibiting LDL oxidation (IC 50 -51.99 μg/mL) and to enhance SOD activity (25.3 %) was higher in ethyl acetate extract (EAP). Phenolic and flavonoid content was measured and showed a positive correlation with antioxidant activity. Presence of apigenin 7, 4'-dimethyl ether (ADE) and piperine (Pip) in EAP was determined by HPTLC analysis and was isolated. ADE inhibited α-glucosidase and α-amylase enzymes and enhanced 2-NBDG uptake in L6 cells. Hypolipidemic effect of ADE on mouse pre-adipocyte (3T3L1) cell lines also showed a dose dependent reduction on lipid droplet content and effective concentration range was determined as 1-2.5 μg/ mL. The results suggested that Piper longum fruits can provide a natural source of antioxidants with antidiabetic and anti obesity potential.

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Section: Adipocyte Differentiation Assaymentioning

confidence: 99%

Effect on oxidative stress, glucose uptake level and lipid droplet content by Apigenin 7, 4'-dimethyl ether isolated from Piper longum L

2014

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“…[48][49][50] Evidence shows the influence of plant compounds in cell-signaling pathways that may lead to regulation of cell differentiation 50 and function. 51 The establishment and function of the human placenta depend on the proliferation, migration, and invasion of trophoblasts into the maternal deciduas and myometrium.…”

Section: Discussionmentioning

confidence: 99%

“…Cells were seeded in 9.2-cm 2 Petri plates (1.8 · 10 5 cell/plate) and incubated for 24 hours in 5% CO 2 and 95% humidity. Then, cells were treated with (1) the MAPK inhibitors PD98059 (50 lM) or SB203580 (10 lM), (2) plant extract (50,250, and 500 lg/mL) with or without FKL (50 lM), (3) FKL (50 lM), and (4) DMSO only (concentration of vehicle present in PD98059/SB203580-treated cells < 0.1%). Doses of MAPK inhibitors were established by dose-dependent tests (data not shown) and reports in the literature.…”

Section: Morphologic Evaluation Of Trophoblast Differentiation By Immmentioning

confidence: 99%

Genipa americana(Rubiaceae) Fruit Extract Affects Mitogen-Activated Protein Kinase Cell Pathways in Human Trophoblast–Derived BeWo Cells: Implications for Placental Development

Conceição

Rossi

Oliveira

et al. 2011

Journal of Medicinal Food

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Genipa americana L. (Rubiaceae) is a fruit tree and a traditional medicine used to treat anemia, icterus, asthma, and liver and spleen problems. The aim of the present study was to verify the effect of G. americana fruit ethanolic extract on the mechanism for proliferation and differentiation of trophoblast-like cells. Qualitative analysis of G. americana fruit extract was performed, and BeWo cells, a well-established placental choriocarcinoma cell line that can undergo differentiation, were used to analyze cell viability and proliferation. Methods consisted of cytotoxic and proliferation measurement, detection of release of human chorionic gonadotrophins, cell fusion observation, and evaluation of cell-signaling pathways (production of cyclic adenosine monophosphate and phosphorylation of mitogen-activated protein kinases [MAPKs]). A stock solution of the extract was diluted in Ham's F-12 medium with 10% fetal bovine serum at concentrations ranging from 50 to 1000 μg/mL. Cells treated with dimethylsulfoxide, forskoline, and MAPK inhibitors (PD98059 or SB203580) were used as a control. Forskoline was used to induce the differentiation state in BeWo cells. Phytoanalysis indicated the presence of steroids only. Results showed that the G. americana fruit extract did not cause any cytotoxicity or interference in cell differentiation. However, a significant antiproliferative state related to inhibition and reactivation of ERK1/2 and p38 MAPK in BeWo cells was seen. These results suggest that steroids from G. americana may affect placental cell regulation.

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“…The PBEE-induced decrease in PPARγ and C/EBPα expression suggested that another PBEE target molecule lay upstream of PPARγ and C/EBPα. To elucidate the signaling pathway through which PBEE affected preadipocyte proliferation, we first examined the possible involvement of ERKs, which have been linked to adipocyte differentiation [23,24]. However, PBEE treatment did not affect ERK phosphorylation.…”

Section: Discussionmentioning

confidence: 99%

A water-soluble extract of Petalonia binghamiae inhibits the expression of adipogenic regulators in 3T3-L1 preadipocytes and reduces adiposity and weight gain in rats fed a high-fat diet

Kang

Kim

Shin

et al. 2010

The Journal of Nutritional Biochemistry

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Hibiscus sabdariffa L. water extract inhibits the adipocyte differentiation through the PI3-K and MAPK pathway

Cited by 73 publications

References 36 publications

Effect on oxidative stress, glucose uptake level and lipid droplet content by Apigenin 7, 4'-dimethyl ether isolated from Piper longum L

Effect on oxidative stress, glucose uptake level and lipid droplet content by Apigenin 7, 4'-dimethyl ether isolated from Piper longum L

Genipa americana(Rubiaceae) Fruit Extract Affects Mitogen-Activated Protein Kinase Cell Pathways in Human Trophoblast–Derived BeWo Cells: Implications for Placental Development

A water-soluble extract of Petalonia binghamiae inhibits the expression of adipogenic regulators in 3T3-L1 preadipocytes and reduces adiposity and weight gain in rats fed a high-fat diet

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