Hierarchical self-assembly of a β-amyloid peptide derivative

Abstract

Neurodegenerative diseases including Alzheimer's, Parkinson's, and type II diabetes are recognized to be related to proteins misfolding into amyloid fibrils and other aggregates with a b-sheet conformation.Herein, self-assembled peptide micro/nanoarchitectures were designed and prepared to mimic those aggregates. A short b-amyloid peptide derivative with a diphenylalanine moiety was synthesized, which could self-assemble into nanofibers via b-sheet conformation in an aqueous solution with a concentration of 1 mg mL À1 at pH about 8. By adjusting the pH to around 6.5, a peptide solution with a concentration of 15 mg mL À1 could change to a supramolecular hydrogel. The influence of selfassembly conditions including peptide concentration, temperature, pressure, and self-assembly time were investigated in detail. It was found that the self-assembled nanofibers could further aggregate into catenulate microfibers in solution as well as layer-by-layer plaques in the hydrogel under particular conditions.

Theranostic GO‐Based Nanohybrid for Tumor Induced Imaging and Potential Combinational Tumor Therapy

“…[ 30,31 ] Butanedioic anhydride was used to link DOX with the N-terminal of the peptide. After the deprotection of the Fmoc group from the Procontaining peptide-resin, butanedioic anhydride (5 equiv.)…”

Semiconducting polymer–dipeptide nanostructures by ultrasonically-assisted self-assembling

“…studied in details the influence of driving forces in this process like peptide concentration, temperature, pressure, and selfassembly time for a β-amyloid peptide derivative. 10 Our attempts using the conventional synthesis protocol were fruitless to produce FF nanostructures with P3HT. To enhance the incorporation of P3HT and also to get control over the shape and size distribution of FF structures we developed a procedure using ultrasound energy to trigger the selfassembling process.…”

Unsaturation‐Dependent Nanostructures Self‐Assembled from Oligopeptide Amphiphiles Capable of Generating Singlet Oxygen

“…After elevating the peptide concentration, all the OPAs could form 3D hydrogel at a suitable concentration. Meanwhile, their critical aggregation concentration (CAC) of OPAs was evaluated by fluorescence spectrometry using pyrene as a hydrophobic probe . The CAC values of OPA1‐3 were 5.3 mg/L, 42.7 mg/L and 5.8 mg/L, respectively (Figure ).…”

“…Inspired by the robust self‐assembly tendency of peptides, peptide‐bearing derivatives have been well designed and exploited to build functional nanostructures. Over the past few decades, a variety of structural motifs such as rigid aromatic rings, active drugs, hydrophilic phosphate, and hydrophobic alkyl chains have been proposed to design various peptide derivatives. Among them, oligopeptide amphiphiles (OPAs) comprising of hydrophilic peptide headgroups and hydrophobic tails are widely employed as building blocks for the self‐assembly and form shape‐specific nanostructures.…”