High cell density cultivation of Escherichia coli with surface anchored transglucosidase for use as whole-cell biocatalyst for α-arbutin synthesis

Wu, Po-Hung; Nair, Giridhar R.; Chu, I‐Ming; Wu, Wen‐Teng

doi:10.1007/s10295-007-0270-0

Cited by 26 publications

(10 citation statements)

References 24 publications

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“…In addition, the biocatalytic ability of E. coli JM109/pet28a-xcas cells was inhibited by a high HQ concentration (208 mM), which is consistent with previous studies [6,7,11,12]. A high concentration of HQ may induce cell apoptosis by changing intracellular redox status through decreasing the thiol level and increasing the reactive oxygen species level [31,32]. To prevent the toxicity of high HQ concentration while retaining α-arbutin production yield, a gradient decreasing fed-batch strategy was applied.…”

Section: Discussionsupporting

confidence: 90%

Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of α-Arbutin

Yang

Zhang

et al. 2018

Catalysts

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α-Arbutin (4-hydroquinone-α-D-glucopyranoside), an effective skin-lightening agent due to its considerable inhibitory effect on human tyrosinase activity, is widely used in the pharmaceutical and cosmetic industries. Recently, α-arbutin was prepared through transglucosylation of hydroquinone using microbial glycosyltransferases as catalysts. However, the low yield and prolonged reaction time of the biotransformation process of α-arbutin production limited its industrial application. In this work, an amylosucrase (ASase) from Xanthomonas campestris pv. campestris str. ATCC 33913 (XcAS) was expressed efficiently in Escherichia coli JM109. The catalytic property of the purified XcAS for the synthesis of α-arbutin was tested. The recombinant strain was applied for highly efficient synthesis of α-arbutin using sucrose and hydroquinone as glucosyl donor and acceptor, respectively. By optimizing the biotransformation conditions and applying a fed-batch strategy, the final production yield and conversion rate of α-arbutin reached 60.9 g/L and 95.5%, respectively, which is the highest reported yield by engineered strains. Compared to the highest reported value (<1.4 g/L/h), our productivity (7.6 g/L/h) was improved more than five-fold. This work represents an efficient and rapid method for α-arbutin production with potential industrial applications.

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Section: Discussionsupporting

confidence: 90%

Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of α-Arbutin

Yang

Zhang

et al. 2018

Catalysts

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“…In this case, the accumulation of glucose and acetate that affected the cell growth was avoided. 23 116.2 g L −1 of succinate was produced at the end of the fermentation by E. coli for the first time. The final OD after 86 h was 35.75.…”

Section: Comparison Of Different Cell Densities At the Transition Poimentioning

confidence: 99%

High cell density fermentation via a metabolically engineered Escherichia coli for the enhanced production of succinic acid

Wang

Song

et al. 2010

J of Chemical Tech & Biotech

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BACKGROUND: Succinic acid is a valuable four-carbon organic chemical with applications in many fields. It was found that cell mass was an important factor in succinic acid production by metabolically engineered Escherichia coli strains. In this work, high cell density fermentation was investigated for succinic acid production by a metabolically engineered strain SD121 with ldhA, pflB, ptsG mutation and heterogenous cyanobacterial ppc overexpression.

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“…For instance, 0.544 g/L arbutin was produced from 49.5 g/L quinol (HQ) when catalyzed by purified dextransucrase [ 12 ]. The whole-cell bioconversion with surface-anchored transglucosidase enabled 21 g/L arbutin production from 200 g/L glucose in high cell density of Escherichia coli under fed-batch fermentation [ 13 ]. Nevertheless, 4.19 g/L arbutin was accumulated in E. coli by plasmid-based expression of biosynthetic enzymes using the shikimate pathway under optimized glucose concentration [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced biosynthesis of arbutin by engineering shikimate pathway in Pseudomonas chlororaphis P3

Wang

Bilal

et al. 2018

Microb Cell Fact

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BackgroundArbutin is a plant-derived glycoside with potential antioxidant, antibacterial and anti-inflammatory activities. Currently, it is mainly produced by plant extraction or enzymatic processes, which suffers from expensive processing cost and low product yield. Metabolic engineering of microbes is an increasingly powerful method for the high-level production of valuable biologicals. Since Pseudomonas chlororaphis has been widely engineered as a phenazine-producing platform organism due to its well-characterized genetics and physiology, and faster growth rate using glycerol as a renewable carbon source, it can also be engineered as the cell factory using strong shikimate pathway on the basis of synthetic biology.ResultsIn this work, a plasmid-free biosynthetic pathway was constructed in P. chlororaphis P3 for elevated biosynthesis of arbutin from sustainable carbon sources. The arbutin biosynthetic pathway was expressed under the native promoter Pphz using chromosomal integration. Instead of being plasmid and inducer dependent, the metabolic engineering approach used to fine-tune the biosynthetic pathway significantly enhanced the arbutin production with a 22.4-fold increase. On the basis of medium factor optimization and mixed fed-batch fermentation of glucose and 4-hydroxybenzoic acid, the engineered P. chlororaphis P3-Ar5 strain led to the highest arbutin production of 6.79 g/L with the productivity of 0.094 g/L/h, with a 54-fold improvement over the initial strain.ConclusionsThe results suggested that the construction of plasmid-free synthetic pathway displays a high potential for improved biosynthesis of arbutin and other shikimate pathway derived biologicals in P. chlororaphis.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-1022-8) contains supplementary material, which is available to authorized users.

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High cell density cultivation of Escherichia coli with surface anchored transglucosidase for use as whole-cell biocatalyst for α-arbutin synthesis

Cited by 26 publications

References 24 publications

Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of α-Arbutin

Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of α-Arbutin

High cell density fermentation via a metabolically engineered Escherichia coli for the enhanced production of succinic acid

Enhanced biosynthesis of arbutin by engineering shikimate pathway in Pseudomonas chlororaphis P3

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