High cell density lipid rich cultivation of a novel microalgal isolate Chlorella sorokiniana FC6 IITG in a single-stage fed-batch mode under mixotrophic condition

Kumar, Vikram; Muthuraj, Muthusivaramapandian; Palabhanvi, Basavaraj; Ghoshal, Aloke Kumar; Das, Debasish

doi:10.1016/j.biortech.2014.07.066

Cited by 56 publications

(18 citation statements)

References 34 publications

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“…7B) at the end of the 5 th batch. The lipid productivity obtained in this study (193.37 mg L -1 day -1 ; Table 4) was higher than that in previous studies with C. sorokiniana grown with glucose or sodium acetate [18,49] or with other microalgal species in mixotrophic conditions [50].…”

Section: Fed-batch Strategy On Biomass and Neutral Lipids Productivitycontrasting

confidence: 70%

“…These observations are in agreement with recent reported studies on the effect of other carbon sources, such as glucose [47], inorganic carbonates [39] or glycerol [20] on lipid productivity in C. sorokiniana. It was also found [18] that acetate acted as an elicitor for lipid enrichment in C. sorokiniana, cultured with glucose as C source.…”

Section: Influence Of Acetate Concentration On Neutral Lipids Productmentioning

confidence: 92%

“…In addition to the standard photoautotrophic growth, many microalgal species can be cultured under heterotrophic conditions with an organic source of carbon and/or energy [15], or under mixotrophic conditions, where they are grown with an organic carbon source in the presence of light [16]. Mixotrophic growth is a mixed approach that combines aspects of both photo-and heterotrophic technologies and a promising alternative for microalgae, capitalizing on the simultaneous assimilation of carbon dioxide and organic carbon sources such as sugars [17,18], acetate [19] or glycerol [20]. However, to achieve economically feasible mixotrophic production it is necessary to choose a cheap organic carbon source.…”

Section: Introductionmentioning

confidence: 99%

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Using agro-industrial wastes for mixotrophic growth and lipids production by the green microalga Chlorella sorokiniana

et al. 2019

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There has been growing interest in the use of microalgae for the production of biofuels, but production costs continue to be too high to compete with fossil fuel prices. One of the main limitations for photobioreactor productivity is light shielding, especially at high cell densities. The growth of the green microalga Chlorella sorokiniana, a robust industrial species, has been evaluated under different trophic conditions with traditional carbon sources, such as glucose and sucrose, and alternative low cost carbon sources, such as carob pod extract, industrial glycerol and acetate-rich oxidized wine waste lees. The mixotrophic cultivation of this microalga with wine waste lees alleviated the problems of light shielding observed in photoautotrophic cultures, improving specific growth rate (0.052 h-1) compared with the other organic sources. The fed-batch mixotrophic culture of Chlorella sorokiniana in a 2 L stirred tank reactor, with optimized nutritional conditions, 100 mM of acetate coming from the oxidized wine waste lees and 30 mM of ammonium, produced an algal biomass concentration of 11 gL-1 with a lipid content of 38% (w/w). This fed-batch strategy has been found to be a very effective means to enhance the biomass and neutral lipid productivity.

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Section: Fed-batch Strategy On Biomass and Neutral Lipids Productivitycontrasting

confidence: 70%

Section: Influence Of Acetate Concentration On Neutral Lipids Productmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Using agro-industrial wastes for mixotrophic growth and lipids production by the green microalga Chlorella sorokiniana

et al. 2019

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“…Glycine, which showed the second highest cell growth rate, forms the dissolved free amino acids in culture medium that can be readily utilized by microalgae (Kumar et al 2014). Some microalgae species, such as Chlorella, were reported to produce a significantly high cell concentration when glycine was the nitrogen source (Li et al 2013.…”

Section: Transesterification and Fame Analysismentioning

confidence: 99%

Effects of nitrogen sources on cell growth and biochemical composition of marine chlorophyte Tetraselmis sp. for lipid production

Kım¹,

Mujtaba²,

Lee³

2016

ALGAE

134

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Nitrogen is one of the most critical nutrients affecting cell growth and biochemical composition of microalgae, ultimately determining the lipid or carbohydrate productivity for biofuels. In order to investigate the effect of nitrogen sources on the cell growth and biochemical composition of the marine microalga Tetraselmis sp., nine different N sources, including NaNO 3 , KNO 3 , NH 4 NO 3 , NH 4 HCO 3 , NH 4 Cl, CH 3 COONH 4 , urea, glycine, and yeast extract were compared at the given concentration of 8.82 mM. Higher biomass concentration was achieved under organic nitrogen sources, such as yeast extract (2.23 g L-1) and glycine (1.62 g L-1), compared to nitrate-(1.45 g L-1) or ammonium-N (0.98 g L-1). All ammonium sources showed an inhibition of cell growth, but accumulated higher lipids, showing a maximum content of 28.3% in ammonium bicarbonate. When Tetraselmis sp. was cultivated using yeast extract, the highest lipid productivity of 36.0 mg L-1 d-1 was achieved, followed by glycine 21.5 mg L-1 d-1 and nitrate 19.9 mg L-1 d-1. Ammonium bicarbonate resulted in the lowest lipid productivity of 14.4 mg L-1 d-1. The major fatty acids in Tetraselmis sp. were palmitic, oleic, linoleic and linolenic acids, regardless of the nutritional compositions, indicating the suitability of this species for biodiesel production.

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“…These act as true storage compounds that 28 are mobilized in the absence of an extracellular carbon source [9], 29 and can, therefore, act as an alternative non-food oil feedstock for 30 the biodiesel industry. These microbial oils differ in composition 31 and properties depending on the organism and substrate used 32 [10,11]. 33 At present, Rhodococcus opacus strain PD630 has been studied 34 mainly to understand the metabolism and biosynthesis of TAGs in 35 bacteria.…”

mentioning

confidence: 99%

Simultaneous lipid production and dairy wastewater treatment using Rhodococcus opacus in a batch bioreactor for potential biodiesel application

Kumar

Gupta

Pakshirajan

2015

Journal of Environmental Chemical Engineering

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High cell density lipid rich cultivation of a novel microalgal isolate Chlorella sorokiniana FC6 IITG in a single-stage fed-batch mode under mixotrophic condition

Cited by 56 publications

References 34 publications

Using agro-industrial wastes for mixotrophic growth and lipids production by the green microalga Chlorella sorokiniana

Using agro-industrial wastes for mixotrophic growth and lipids production by the green microalga Chlorella sorokiniana

Effects of nitrogen sources on cell growth and biochemical composition of marine chlorophyte Tetraselmis sp. for lipid production

Simultaneous lipid production and dairy wastewater treatment using Rhodococcus opacus in a batch bioreactor for potential biodiesel application

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