High-density mapping of brain slices using a large multi-functional high-density CMOS microelectrode array system

Abstract

We present a CMOS-based high-density microelectrode array (HD-MEA) system that enables high-density mapping of brain slices in-vitro with multiple readout modalities. The 4.48×2.43 mm 2 array consists of 59,760 micro-electrodes at 13.5 µm pitch (5487 electrodes/mm 2 ). The overall system features 2048 action-potential, 32 local-field-potential and 32 current recording channels, 32 impedance-measurement and 28 neurotransmitter-detection channels and 16 voltage/ current stimulation channels. The system enables real-time and label-free monitoring of position, size, morphology and electrical activity of brain slices.

Large-area electrical imaging having single neuron resolution using 236,880 electrodes CMOS-MEA technology

“…The data and scripts that support the findings of this study are available from the corresponding author upon reasonable request. (D) Comparison of the number of electrodes to the measurement area 14,15,77,79,133,134,135,136,137,138,139,140,141,142,143 . (E) Comparison of the reciprocal of the electrode size to the number of electrodes per measurement area 14,15,77,133,134,135,136,137,138,140,141,142,143 .…”

“…(C) Electron microscope image of single electrode size (11.22 µm × 11.22 µm, pitch＝0.25 µm). (D) Comparison of the number of electrodes to the measurement area14,15,77,79,133,134,135,136,137,138,139,140,141,142,143 . (E) Comparison of the reciprocal of the electrode size to the number of electrodes per measurement area14,15,77,133,134,135,136,137,138,140,141,142,143 .…”

Xenon LFP Analysis Platform Is a Novel Graphical User Interface for Analysis of Local Field Potential From Large-Scale MEA Recordings

“…The technology of neuronal data acquisition using high density multi-electrode arrays (HD-MEAs) in tissue and cell cultures has grown dramatically over the past decade (Maccione et al, 2013(Maccione et al, , 2014(Maccione et al, , 2015Ingebrandt, 2015;Müller et al, 2015;Dragas et al, 2017;Viswam et al, 2017;Steinmetz et al, 2019;Paulk et al, 2022). A brief history of MEA technology and advancement of these devices is discussed extensively by Didier et al (2020).…”

Assessing the Feasibility of Developing in vivo Neuroprobes for Parallel Intracellular Recording and Stimulation: A Perspective

“…Rather, it appears that the in vivo neuroengineering/neuroscience communities are focusing their efforts on developing high density extracellular MEA for in vivo use. These efforts led to very successful realization of MEA platforms carrying thousands of high-density, small diameter low impedance and addressable electrodes (for example, Jackel et al, 2017 ; Jun et al, 2017 ; Raducanu et al, 2017 ; Viswam et al, 2017 ; Dorigo et al, 2018 ; Angotzi et al, 2019 ; Steinmetz et al, 2021 ) or the development of highly efficient robotic tools to implant thousands of individual electrodes ( Musk, 2019 ). In spite of the immense progress, the new generations of dense extracellular in vivo MEAs relies on the use of planar electrodes and suffers from the innate limitations of being “blind” to subthreshold electrophysiological signals, low signal-to-noise ratio, low source resolution and deterioration of the recording yield and FP amplitudes within days to weeks after implantation.…”