High‐Density Peptide Arrays with Combinatorial Laser Fusing

Combinatorial Particle Patterning

“…If the particles are fused to a surface via laser irradiation, their adhesion is greater than those that are not processed with the laser. This fact is exploited in the CLF approach ( Figure a) . Combinatorial patterning of different particles was realized by repetitive deposition of particles with an advanced aerosol generation and deposition technique, followed by melting them with the laser and the subsequent removal of loose particles .…”

“…The image area is 1.5 × 1.5 cm 2 resulting in 90 000 spots with a spot density of 40 000 spots per cm 2 . Reproduced with permission . Copyright 2014, John Wiley and Sons.…”

Selective Functionalization of Microstructured Surfaces by Laser‐Assisted Particle Transfer

“…Also lithographic synthesis methods, [7] based on These particle deposition techniques are limited to 800 spots per cm 2 for xerographic approaches [11] and 40,000 spots per cm 2 for combinatorial laser fusing. [10] Here, we present a laser-assisted particle transfer method for the selective functionalization of up to 1 million microcavities per cm². In our approach, we use styrene acrylic copolymer microparticles with embedded pre-activated monomers, which are selectively transferred into microcavities of a microstructured glass surface.…”

Replication of Polymer‐Based Peptide Microarrays by Multi‐Step Transfer

“…[4] Itsb asicp rinciple is to freeze activated aminoa cids within polymerp articles,w hich aret hend eposited on topo f ap olymer-baseds ubstrate by usinge itheralaserp rinter [5] or ac omplementarym etal oxides emiconductor (CMOS) chip. [6] A newa pproache mploys al aser to immobilize particlesi nc ertain spotso nt op of thes ynthesiss urface,y ieldingf eature sizesa s smalla s1 0mm. [6] Allt hese techniques,h owever,s hare thei ssue of varyingp eptide concentrations within differents pots,a ss yn-thesis yields arev eryd ependent on thes equenceo ft he peptide.T hisr esults in an increasing amount of incomplete peptideswithincreasingpeptide length.…”

“…[6] A newa pproache mploys al aser to immobilize particlesi nc ertain spotso nt op of thes ynthesiss urface,y ieldingf eature sizesa s smalla s1 0mm. [6] Allt hese techniques,h owever,s hare thei ssue of varyingp eptide concentrations within differents pots,a ss yn-thesis yields arev eryd ependent on thes equenceo ft he peptide.T hisr esults in an increasing amount of incomplete peptideswithincreasingpeptide length. [7] The efficiencies of differenta mino acid couplings range from 92 %t oa sl ow as 42 %.…”