High Energy Shock Waves Activate 5′-Aminolevulinic Acid and Increase Permeability to Paclitaxel: Antitumor Effects of a New Combined Treatment on Anaplastic Thyroid Cancer Cells

Catalano, Maria Graziella; Costantino, Lucia; Fortunati, Nicoletta; Bosco, Ornella; Pugliese, Mariateresa; Boccuzzi, Giuseppe; Berta, Laura; Frairia, Roberto

doi:10.1089/thy.2006.0142

Cited by 19 publications

(14 citation statements)

References 39 publications

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“…Indeed, we here demonstrated that NB entrance was significantly increased by ESW treatment. Accordingly, it has already been reported that ESWs increase cell permeability [15,16,18], affecting drug efficacy in breast cancer cells [14], in anaplastic thyroid cancer cells [18], and in an in vivo breast cancer model [19]. Therefore, NBs can act as stable taxane reservoir in the castration-resistant prostate cancer cells and ESWs further increase drug release from NBs, leading to higher cytotoxic and anti-migration effects.…”

Section: Discussionmentioning

confidence: 99%

“…We recently reported elsewhere [17], that ESWs enhance cytotoxicity of doxorubicin-loaded glycol chitosan NBs in anaplastic thyroid cancer cells. Moreover, ESWs increase paclitaxel-induced apoptosis in breast cancer cell lines [14], in anaplastic thyroid cancer cells [18] and in a Mat B-III rat syngeneic model of breast cancer [19]. Lastly, they enhance the cytotoxic effect of doxorubicin and methotrexate in human osteosarcoma cell lines [20].…”

Section: Introductionmentioning

confidence: 99%

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Targeting Taxanes to Castration-Resistant Prostate Cancer Cells by Nanobubbles and Extracorporeal Shock Waves

et al. 2016

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To target taxanes to castration-resistant prostate cancer cells, glycol-chitosan nanobubbles loaded with paclitaxel and docetaxel were constructed. The loaded nanobubbles were then combined with Extracorporeal Shock Waves, acoustic waves widely used in urology and orthopedics, with no side effects. Nanobubbles, with an average diameter of 353.3 ± 15.5 nm, entered two different castration-resistant prostate cancer cells (PC3 and DU145) as demonstrated by flow cytometry and immunofluorescence. The shock waves applied increased the amount of intracellular nanobubbles. Loading nanobubbles with paclitaxel and docetaxel and combining them with shock waves generated the highest cytotoxic effects, resulting in a paclitaxel GI50 reduction of about 55% and in a docetaxel GI50 reduction of about 45% respectively. Combined treatment also affected cell migration. Paclitaxel-loaded nanobubbles and shock waves reduced cell migration by more than 85% with respect to paclitaxel alone; whereas docetaxel-loaded nanobubbles and shock waves reduced cell migration by more than 82% with respect to docetaxel alone. The present data suggest that nanobubbles can act as a stable taxane reservoir in castration-resistant prostate cancer cells and shock waves can further increase drug release from nanobubbles leading to higher cytotoxic and anti-migration effect.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Targeting Taxanes to Castration-Resistant Prostate Cancer Cells by Nanobubbles and Extracorporeal Shock Waves

et al. 2016

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“…Extracorporeal shock waves (ESWs) are short-duration focused acoustic waves widely used in urology for lithotripsy (Rassweiler et al 2011) and, more recently, in several musculoskeletal diseases (Wang 2012). ESWs can be focused with high precision in depth, and they determine permeabilization of plasma membranes (Lauer et al 1997, Kodama et al 2002, Frairia et al 2003, Catalano et al 2007. As a consequence of ESWinduced cell permeability, ESWs increase the cytotoxic effects of different anti-cancer drugs (Frairia et al 2003, Palmero et al 2006, Catalano et al 2007, Canaparo et al 2008.…”

Section: :6mentioning

confidence: 99%

“…ESWs can be focused with high precision in depth, and they determine permeabilization of plasma membranes (Lauer et al 1997, Kodama et al 2002, Frairia et al 2003, Catalano et al 2007. As a consequence of ESWinduced cell permeability, ESWs increase the cytotoxic effects of different anti-cancer drugs (Frairia et al 2003, Palmero et al 2006, Catalano et al 2007, Canaparo et al 2008. All these features make ESWs an ideal tool to be used in combination with drug-loaded NBs.…”

Section: :6mentioning

confidence: 99%

Combining doxorubicin-nanobubbles and shockwaves for anaplastic thyroid cancer treatment: preclinical study in a xenograft mouse model

Marano¹,

Frairia²,

Rinella³

et al. 2017

Endocrine-Related Cancer

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Anaplastic thyroid cancer is one of the most lethal diseases, and a curative therapy does not exist. Doxorubicin, the only drug approved for anaplastic thyroid cancer treatment, has a very low response rate and causes numerous side effects among which cardiotoxicity is the most prominent. Thus, doxorubicin delivery to the tumor site could be an import goal aimed to improve the drug efficacy and to reduce its systemic side effects. We recently reported that, in human anaplastic thyroid cancer cell lines, combining doxorubicin-loaded nanobubbles with extracorporeal shock waves, acoustic waves used in lithotripsy and orthopedics without side effects, increased the intracellular drug content and cytotoxicity. In the present study, we tested the efficacy of this treatment on a human anaplastic thyroid cancer xenograft mouse model. After 21 days, the combined treatment determined the greatest drug accumulation in tumors with consequent reduction of tumor volume and weight, and an extension of the tumor doubling time. Mechanistically, the treatment induced tumor apoptosis and decreased cell proliferation. Finally, although doxorubicin caused the increase of fibrosis markers and oxidative stress in animal hearts, loading doxorubicin into nanobubbles avoided these effects preventing heart damage. The improvement of doxorubicin anti-tumor effects together with the prevention of heart damage suggests that the combination of doxorubicin-loaded nanobubbles with extracorporeal shock waves might be a promising drug delivery system for anaplastic thyroid cancer treatment.

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“…Furthermore, encapsulation in NPs provided other pathways for uptake and intracellular release of the drug. The hydrophobic Paclitaxel in solution is taken up by tumor cells primarily by diffusion through the cell membrane (58,59). On the other side, Paclitaxel loaded NPs attach to tumor cell membrane (although the extent of attachment in the absence of specific ligand-receptor interaction is low), which minimizes the gradient between the extracellular matrix and cell surface, and are internalized by pinocytosis or phagocytosis.…”

Section: Tumor Cell Viabilitymentioning

confidence: 99%

Cytotoxicity of Paclitaxel in Biodegradable Self-Assembled Core-Shell Poly(Lactide-Co-Glycolide Ethylene Oxide Fumarate) Nanoparticles

Mercado

et al. 2008

Pharm Res

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Groups with Paclitaxel loaded NPs had higher cytotoxicity compared to Paclitaxel directly added to the media at the same concentration. NPs acted as reservoirs to protect the drug from epimerization and hydrolysis while providing a sustained dose of Paclitaxel with time. Infrared image of the Apc(Min/+) mouse injected with NPs showed significantly higher concentration of NPs in the intestinal tissue.

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High Energy Shock Waves Activate 5′-Aminolevulinic Acid and Increase Permeability to Paclitaxel: Antitumor Effects of a New Combined Treatment on Anaplastic Thyroid Cancer Cells

Abstract: These preliminary data suggest the possibility of using HESW and ALA in combination with paclitaxel as a promising new therapy in the treatment of anaplastic thyroid cancer.

Cited by 19 publications

References 39 publications

Targeting Taxanes to Castration-Resistant Prostate Cancer Cells by Nanobubbles and Extracorporeal Shock Waves

Targeting Taxanes to Castration-Resistant Prostate Cancer Cells by Nanobubbles and Extracorporeal Shock Waves

Combining doxorubicin-nanobubbles and shockwaves for anaplastic thyroid cancer treatment: preclinical study in a xenograft mouse model

Cytotoxicity of Paclitaxel in Biodegradable Self-Assembled Core-Shell Poly(Lactide-Co-Glycolide Ethylene Oxide Fumarate) Nanoparticles

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