High-frequency adventitious shoots regeneration from leaf of non-heading Chinese cabbage (Brassica campestris ssp. chinensis) cultured in vitro

Memon, Saba Ambreen; Hou, Xilin; Zhu, Bo; Wolukau, Joseph N.

doi:10.1007/s11738-009-0353-9

Cited by 9 publications

(6 citation statements)

References 9 publications

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“…It has also be found that addition of L-glutamine into the co-cultivation medium allowed the efficient Agrobacterium tumefaciens mediated transformation of tea by avoiding the bactericidal effect of leaf polyphenols produced by leaf tissues (Sandal et al, 2007). Addition of silver nitrate (AgNO 3 ), also known as an ethylene inhibitor, into the basal medium has significantly enhanced shoot production (Memon et al, 2009) and rooting (Dai et al, 2009); however, it has also been found that it can increase ethylene synthesis under in vitro cultures with several morphogenic responses but without basic molecular explanations (Zhang et al, 1998;Kumar et al, 2009).…”

Section: Stage I Introduction and Establishmentmentioning

confidence: 99%

Plant tissue culture: Current status, opportunities and challenges

et al. 2010

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R. García-Gonzáles, K. Quiroz, B. Carrasco, and P.D.S. Caligari. 2010. Plant tissue culture: Current status, opportunities and challenges. Cien. Inv. Agr. 37(3): 5-30. In the last two decades plant biotechnology applications have been widely developed and incorporated into the agricultural systems of many countries worldwide. Tissue culture tools have been a key factor to support such outcomes. Current results have allowed plant biotechnology and its products-including transgenic plants with several traits-to be the most assimilated technology for farmers and companies, representing several benefits such as: 125 millions ha of transgenic crops in 2008, the reduction of pesticides application by up to 9% in the last ten years, transgenic plants with a better nutritional quality, mass propagation of selected and healthy plants, and the production of proteins for industrial or therapeutic use. The rapid and extensive assimilation for this technology has improved the competences of the agricultural systems both in industrial and in developing countries, based on the proper application of research programs. Several theoretical and practical aspects supporting plant tissue culture applications, as well as the main results and current status of the technology are discussed in this review. The reader will find key elements to evaluate the potential of plant tissue culture tools for the development of agriculture, livestock, human health and nutrition, and human well being in general.

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Section: Stage I Introduction and Establishmentmentioning

confidence: 99%

Plant tissue culture: Current status, opportunities and challenges

et al. 2010

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“…The dose of cytokinin in the culture medium was sufficient to induce shoots in crambe, which is critical for effective organogenesis. The effect of BAP on the induction of multiple shoots has been previously reported in species of the family Brassicaceae (Shukla and Sawhney, 1991;Memon et al, 2009), but no studies have described the effect of this regulator over time in vitro cultures. Means followed by the same letter in the column do not differ by Tukey's test at 5% CV(%): percent coefficient of variation.…”

Section: Resultsmentioning

confidence: 98%

“…Means followed by the same lowercase letter in the columns do not differ by Tukey's test at 5%. Memon et al (2009) reported 90% rooting of Brassica campestris shoots in MS medium supplemented with 1 µM ANA, whereas only 54.4% rooting was observed when the medium was supplemented with 2.5 µM ANA. The results of Li et al (2010) using adventitious shoots generated from the hypocotyls of the C. abyssinica cultivar Galactica were not similar to those reported in the present study.…”

Section: Resultsmentioning

confidence: 99%

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Werner¹,

Soares²,

Gontijo³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Crambe (Crambe abyssinica) is a non-edible annual herb, which was first cultivated to extract oil for industry, and now has great potential for biodiesel production. The objective of this investigation was to evaluate the genetic stability of micropropagated plants of the C. abyssinica Hochst cultivar 'FMS brilhante' using polymerase chain reaction techniques based on inter-simple sequence repeat (ISSR) molecular markers. The aim was to develop a protocol for the in vitro regeneration of these plants with low genetic variation as compared to the donor plant. For micropropagation, shoot tips from in vitro germinated seedlings were used as explants and were initially cultivated for 90 days on MS medium with 5.0 µM 6-benzylaminopurine (BAP), which at 90 days, led to the highest number of shoots per explant (NSE) (12.20 shoots) being detected. After 120 days, the interaction between BAP concentration and naphthalene acetic acid (NAA) was tested, and the highest NSE was observed following exposure to 0.0/0.5 µM BAP/NAA (11.40 shoots) and 1.0/0.0 µM BAP/NAA (11.00 shoots). The highest proportion of rooting phase were observed following exposure to 0.5 µM NAA (30%). The 13 ISSR primers used to analyze genetic stability produced 91 amplification products, of which only eight bands were polymorphic and 83 were monomorphic for all 10 regenerated crambe plants, compared to the donor plant explant. These results indicate that crambe shoot tips are a highly reliable explant that can be used to micropropagate genetically true-to-type plants or to maintain genetic stability, as verified using ISSR markers.

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“…BAP is one of the cytokinins with lowest cost, and has been very efficacious in the multiplication of diverse Brassicaceae species (Shukla and Sawhney, 1991;Memon et al, 2009;Ishigaki et al, 2009). Studies related to induction of shoots and regeneration of whole plants from apical segments have not yet been described for crambe.…”

Section: Shoot Multiplicationmentioning

confidence: 99%

“…For this, genetic transformation and in vitro culture techniques are important tools to be employed. However, the genetic transformation of plants demands the successful application of these techniques, requiring an efficient, viable, genetically loyal propagation system that provides plant material of good quality to satisfy this demand (Memon et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Crambe Abyssinica Hochst. Ex R.E. Fr.

Werner¹,

Gontijo²,

Amaral³

et al. 2017

Aust J Crop Sci

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Crambe abyssinica Hochst. Ex. R. E. Fr. is an oilseed species that produces non-edible oil, initially employed in Brazil as a culture for soil surface coverage and animal feed. However, with stimulation of the production and use of renewable energies, it arose as an interesting alternative for biodiesel production. The aim of this work was to describe a protocol for micropropagation of crambe, using apical segments as explants. Crambe seeds germinated in vitro in medium MS½ provided apical segments adequate to the micropropagation process. In the shoot induction phase, testing the interaction of the cytokinins 6-benzylaminopurine (BAP), kinetin (KIN) and thidiazuron (TDZ) as well as different concentrations thereof, the highest numbers of shoots per explant were obtained with 5 µM BAP at 30 days (9.26 shoots) and 15 µM BAP at 60 days (18.08 shoots) of in vitro culture. In the elongation with 1 µM gibberellic acid (GA 3 ), the mean length of the shoots turned from 3.61 cm to 5.03 cm after 30 days. The highest percentage of rooting was of 30% with 0.25 µM naphthalene-acetic acid (NAA). The results of this work revealed a satisfactory frequency of shoot regeneration from apical segments of crambe in media containing 5 µM BAP, and adequate elongation with 1 µM GA 3 . Nevertheless, there is still need for efficient rooting and acclimatization for complete in vitro propagation of crambe.

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High-frequency adventitious shoots regeneration from leaf of non-heading Chinese cabbage (Brassica campestris ssp. chinensis) cultured in vitro

Cited by 9 publications

References 9 publications

Plant tissue culture: Current status, opportunities and challenges

Plant tissue culture: Current status, opportunities and challenges

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Micropropagation of Crambe Abyssinica Hochst. Ex R.E. Fr.

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