High-frequency, dielectric spectroscopy for the detection of electrophysiological/biophysical differences in different bacteria types and concentrations

Russel, Mohammad; Sophocleous, Marios; Shan, Jiajia; Xu, Weiping; Xiao, Lehui; Maskow, Thomas; Alam, Md. Mahbub; Georgiou, Julius

doi:10.1016/j.aca.2018.04.045

Cited by 18 publications

(4 citation statements)

References 60 publications

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“…Following the studies on dielectric spectroscopy for cells and bacteria [41], [42], [43], SRRbased sensors have been developed for real-time monitoring of bacteria concentration and growth [44], [45], [46] and the effect of glucose or antibiotics on this growth [47], [48]. As SRRs have also been implemented in tissue studies, focusing on the tissues' dielectric characteristics as a key feature of their physiological state.…”

Section: Split Ring Resonatorsmentioning

confidence: 99%

Dielectric Spectroscopy: Revealing the True Colors of Biological Matter

et al. 2023

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Accurate characterization of biological matter, for example, in tissue, cells, and biological fluids, is of high importance. For example, early and correct detection of abnormalities, such as cancer, is essential as it enables early and effective type-specific treatment, which is crucial for mortality reduction [1]. Moreover, it is imperative to investigate the effectiveness and toxicity of pharmaceutical treatments before administration in clinical practice [2]. However, biological matter characterization still faces many challenges. State-of-the-art imaging and characterization methods have drawbacks, such as the requirement to attach difficult-to-find and costly labels to the biological target (e.g., COVID-19 rapid test), expensive equipment (e.g., magnetic resonance imaging or MRI), low accuracy (e.g., ultrasound), use of ionizing radiation (e.g., X-rays), and invasiveness [3]. The characterization of biological matter using microwave (µW), millimeter wave (mmW), and Terahertz (THz) spectroscopy is a promising alternative: it is label-free, does not require ionizing radiation, and can be non-invasive.Moreover, there is a significant difference in how different biological materials absorb, reflect, and transmit electromagnetic (EM) waves [4] that is due to the difference in their dielectric properties. The dielectric properties are described by the frequency-dependent material parameter called the complex permittivity 𝜺(𝒇), which expresses how the material

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Section: Split Ring Resonatorsmentioning

confidence: 99%

Dielectric Spectroscopy: Revealing the True Colors of Biological Matter

et al. 2023

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“…Key assumptions made in the model formulation are addressed in the following section. Dielectric properties of B. subtilis are applied to the particle [16] and the fluid medium is specified as DI water. All parameters used in the simulation are provided in Table 1.…”

Section: Simulation Of Dep Force and Particle Tracingmentioning

confidence: 99%

Bacteria and cancer cell pearl chain under dielectrophoresis

et al. 2021

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In this work, we aim to observe and study the physics of bacteria and cancer cells pearl chain formation under dielectrophoresis (DEP). Experimentally, we visualized the formation of Bacillus subtilis bacterial pearl chain and human breast cancer cell (MCF-7) chain under positive and negative dielectrophoretic force, respectively. Through a simple simulation with creeping flow, AC/DC electric fields, and particle tracing modules in COMSOL, we examined the mechanism by which bacteria self-organize into a pearl chain across the gap between two electrodes via DEP. Our simulation results reveal that the region of greatest positive DEP force shifts from the electrode edge to the leading edge of the pearl chain, thus guiding the trajectories of free-flowing particles toward the leading edge via positive DEP. Our findings additionally highlight the mechanism why the free-flowing particles are more likely to join the existing pearl chain rather than starting a new pearl chain. This phenomenon is primarily due to the increase in magnitude of electric field gradient, and hence DEP force exerted, with the shortening gap between the pearl chain leading edge and the adjacent electrode. The findings shed light on the observed behavior of preferential pearl chain formation across electrode gaps.

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“…Clostridium difficile, sensitivity 95% and specificity 95% [90] Monoclonal antibodies Surface plasmon resonance M. tuberculosis, 10 2 -10 6 ufc/mL [93,101] Aptamer Fluorescent S. enterica Typhimurium, 6-10 ufc/mL [92] Monoclonal antibodies Potentiometry S. enterica Typhimurium, 6 ufc/mL [91] Nucleic acids (DNA) Electrochemical impedance M. tuberculosis, 10 2 -10 6 ufc/mL [50] Aptamer (RNA) Fluorescent S. aureus, 10 2 -10 6 ufc/mL [3] Nicolson-Ross-Weir method Dielectric spectroscopy Bacillus Subtilis, 2.10-1.30 × 10 9 ufc/mL E. coli 1.60-1.00 × 10 9 ufc/mL [106] Note: * Man/MUA-MH/Au-mannose/11-Mercaptoundecanoic acid/6-mercapto-hexanol/gold.…”

Section: Electrochemical Label-free Biosensorsmentioning

confidence: 99%

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

et al. 2020

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Infections pose a serious global public health problem and are a major cause of premature mortality worldwide. One of the most challenging objectives faced by modern medicine is timely and accurate laboratory-based diagnostics of infectious diseases. Being a key factor of timely initiation and success of treatment, it may potentially provide reduction in incidence of a disease, as well as prevent outbreak and spread of dangerous epidemics. The traditional methods of laboratory-based diagnostics of infectious diseases are quite time- and labor-consuming, require expensive equipment and qualified personnel, which restricts their use in case of limited resources. Over the past six decades, diagnostic technologies based on lateral flow immunoassay (LFIA) have been and remain true alternatives to modern laboratory analyzers and have been successfully used to quickly detect molecular ligands in biosubstrates to diagnose many infectious diseases and septic conditions. These devices are considered as simplified formats of modern biosensors. Recent advances in the development of label-free biosensor technologies have made them promising diagnostic tools that combine rapid pathogen indication, simplicity, user-friendliness, operational efficiency, accuracy, and cost effectiveness, with a trend towards creation of portable platforms. These qualities exceed the generally accepted standards of microbiological and immunological diagnostics and open up a broad range of applications of these analytical systems in clinical practice immediately at the site of medical care (point-of-care concept, POC). A great variety of modern nanoarchitectonics of biosensors are based on the use of a broad range of analytical and constructive strategies and identification of various regulatory and functional molecular markers associated with infectious bacterial pathogens. Resolution of the existing biosensing issues will provide rapid development of diagnostic biotechnologies.

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High-frequency, dielectric spectroscopy for the detection of electrophysiological/biophysical differences in different bacteria types and concentrations

Cited by 18 publications

References 60 publications

Dielectric Spectroscopy: Revealing the True Colors of Biological Matter

Dielectric Spectroscopy: Revealing the True Colors of Biological Matter

Bacteria and cancer cell pearl chain under dielectrophoresis

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

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