High frequency ultrasonic backscatter from erythrocyte suspension

Kuo, I.Y.; Shung, K. Kirk

doi:10.1109/10.277268

Cited by 31 publications

(19 citation statements)

References 17 publications

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“…This scatterer density is high in blood, and therefore it makes ultrasonic blood characterization difficult. A nonlinear relationship has been found between backscattering power and scatterer concentration in an extremely dense medium (such as blood) [17]. Backscattering is dependent on an acoustic impedance mismatch between the tissue and cell components [12].…”

Section: Introductionmentioning

confidence: 99%

A pulsed photoacoustic technique for studying red blood cell sedimentation

Kinnunen

2015

JBPE

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Abstract. This study shows the capability of a pulsed photoacoustic (PA) technique to measure red blood cell sedimentation and aggregation processes in vitro. Red blood cells are the main source of absorption in blood. The PA signal is proportional to the sample's optical absorption coefficient, and hence, dynamic changes in the sample can be monitored by analyzing the PA pulse amplitude and pulse arrival time. Optical coherence tomography (OCT) is used as a parallel method for comparison. Diluted whole blood and different concentrations of washed red blood cells were used as samples. The pulsed PA technique is suitable for monitoring changes in sedimentation velocity when dextran is added to the sample. When the measurement section with the fastest sedimentation rate was selected for analysis, a more than 10--fold increase in the sedimentation rate, induced by dextran, was found with both the PA and OCT techniques. The PA pulse delay was found to be a more reliable measure of changes in the sample than the PA signal amplitude. © 2015 Samara State Aerospace University (SSAU).

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Section: Introductionmentioning

confidence: 99%

A pulsed photoacoustic technique for studying red blood cell sedimentation

Kinnunen

2015

JBPE

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“…Under the condition ka < 1, ultrasound backscattering is not affected by the shape of scatterers [30]. Savery and Cloutier reported that ultrasound backscattering behavior of RBCs as a function of frequency is very similar to Rayleigh scattering below 87 MHz with analytical and semi-analytical models study [31].…”

Section: Resultsmentioning

confidence: 99%

Ultrasonic scattering measurements of a live single cell at 86 MHz

Lee

Jung

Lam

et al. 2015

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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Cell separation and sorting techniques have been employed biomedical applications such as cancer diagnosis and cell gene expression analysis. The capability to accurately measure ultrasonic scattering properties from cells is crucial in making an ultrasonic cell sorter a reality if ultrasound scattering is to be used as the sensing mechanism as well. To assess the performance of sensing and identifying live single cells with high-frequency ultrasound, an 86-MHz lithium niobate press-focused single-element acoustic transducer was used in a high-frequency ultrasound scattering measurement system that was custom designed and developed for minimizing noise and allowing better mobility. Peak-to-peak echo amplitude, integrated backscatter (IB) coefficient, spectral parameters including spectral slope and intercept, and midband fit from spectral analysis of the backscattered echoes were measured and calculated from a live single cell of two different types on an agar surface: leukemia cells (K562 cells) and red blood cells (RBCs). The amplitudes of echo signals from K562 cells and RBCs were 48.25 ± 11.98 mVpp and 56.97 ± 7.53 mVpp, respectively. The IB coefficient was −89.39 ± 2.44 dB for K562 cells and −89.00 ± 1.19 dB for RBCs. The spectral slope and intercept were 0.30 ± 0.19 dB/MHz and −56.07 ± 17.17 dB, respectively, for K562 cells and 0.78 ± 0.092 dB/MHz and −98.18 ± 8.80 dB, respectively, for RBCs. Midband fits of K562 cells and RBCs were −31.02 ± 3.04 dB and −33.51 ± 1.55 dB, respectively. Acoustic cellular discrimination via these parameters was tested by Student’s t-test. Their values, except for the IB value, showed statistically significant difference (p < 0.001). This paper reports for the first time that ultrasonic scattering measurements can be made on a live single cell with a highly focused high-frequency ultrasound microbeam at 86 MHz. These results also suggest the feasibility of ultrasonic scattering as a sensing mechanism in the development of ultrasonic cell sorters.

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“…The T-matrix formulation for a sphere was used to compare with its experimental results. T mv , an indexed element in the matrix, was defined as the ratio of scattering field coefficients

Q_{m v}^{s}

to incident field coefficients

Q_{m v}^{i}

and written as [22]…”

Section: Methodsmentioning

confidence: 99%

Backscattering measurement from a single microdroplet

Lee

Chang

Jeong

et al. 2011

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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Backscattering measurements for acoustically trapped lipid droplets were undertaken by employing a P[VDF-TrFE] broadband transducer of f-number = 1, with a bandwidth of 112%. The wide bandwidth allowed the transmission of the 45 MHz trapping signal and the 15 MHz sensing signal using the same transducer. Tone bursts at 45 MHz were first transmitted by the transducer to hold a single droplet at the focus (or the center of the trap) and separate it from its neighboring droplets by translating the transducer perpendicularly to the beam axis. Subsequently, 15 MHz probing pulses were sent to the trapped droplet and the backscattered RF echo signal received by the same transducer. The measured beam width at 15 MHz was measured to be 120 μm. The integrated backscatter (IB) coefficient of an individual droplet was determined within the 6-dB bandwidth of the transmit pulse by normalizing the power spectrum of the RF signal to the reference spectrum obtained from a flat reflector. The mean IB coefficient for droplets with a 64 μm average diameter (denoted as cluster A) was −107 dB, whereas it was −93 dB for 90-μm droplets (cluster B). The standard deviation was 0.9 dB for each cluster. The experimental values were then compared with those computed with the T-matrix method and a good agreement was found: the difference was as small as 1 dB for both clusters. These results suggest that this approach might be useful as a means for measuring ultrasonic backscattering from a single microparticle, and illustrate the potential of acoustic sensing for cell sorting.

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High frequency ultrasonic backscatter from erythrocyte suspension

Cited by 31 publications

References 17 publications

A pulsed photoacoustic technique for studying red blood cell sedimentation

A pulsed photoacoustic technique for studying red blood cell sedimentation

Ultrasonic scattering measurements of a live single cell at 86 MHz

Backscattering measurement from a single microdroplet

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