Traditional methods for measuring acoustic speed require knowledge of either the specimen thickness or the distances between the transducers and the specimen. In general, the accuracy in measuring these quantities determines the accuracy of the experimental technique for measuring speed. This problem is particularly acute in measuring sound speed in biological specimens. A new method for measuring acoustic speed of materials, which eliminates the need for determining these quantities, has been developed. The technique, which necessitates the use of only one transducer, requires measurement of four times of flight of a sound pulse and the knowledge of the speed of sound in a reference fluid medium in which the specimen is placed. Ultrasonic speed in stainless steel and Plexiglas was measured using this method to verify its validity. Results on measurements on porcine liver, myocardium, and soft fat are also reported.

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Previous studies have shown that ultrasonic backscattering from red blood cells suspended in saline is proportional to the fourth power of frequency for frequencies below 15 MHz, as predicted by Rayleigh scattering theory. Recently, we have extended the measurements up to 30 MHz, because scattering of ultrasound by red blood cells may no longer be negligible at these frequencies and can affect, to a great degree, the operation of intravascular imaging devices. The experimental results show that the fourth power dependence on frequency of the backscattering coefficient for porcine erythrocytes suspended in saline solution appears to be valid up to 30 MHz. To confirm this, backscattering cross-section of porcine red cells was computed as a function of frequency using the T-matrix method. Since at higher frequencies the shape of the scatterers may also play a significant role, its effect was investigated by treating the red cell as a sphere, a disc, and a biconcave disc of the same volume. Good agreement was obtained between the experimental and theoretical results.

The backscattering coefficient of saline suspensions of porcine red blood cells was measured for hematocrits up to about 90%. It was found that the coefficient peaks at approximately 15%, but then, contrary to what a simple "gap theory" might suggest, it decays smoothly to zero, without showing another peak at high hematocrits. A one-dimensional (1-D) slab scattering model, in which the number of slabs per unit length represents the hematocrit and whose thickness and acoustical properties are similar to red cells/plasma, was also used to investigate the relation between the backscattered power and hematocrit. Monte-Carlo simulations performed for randomized boundary conditions show a similar relation to that of the 3-D system. The experimental data is compared to the Percus-Yevick theory for the packing of hard spheres, and the simulated data is compared to the Percus-Yevick theory for infinite slabs.

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