High Glycemic Index Diet Decreases Insulin Secretion without Altering Akt and Pdx1 Expression on Pancreatic Beta Cells in Mice

Herawati, Lilik; Sari, Gadis Meinar; Irawan, Roedi

doi:10.12982/cmujns.2020.0024

Cited by 10 publications

(10 citation statements)

References 20 publications

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“…The incidence of insulin resistance is indirectly related to an unhealthy lifestyle, such as excessive calorie consumption. In a previous study by Herawati et al [9] , mice that received a high-glucose diet experienced a higher increase in blood sugar levels than the control group and had a lower density of beta cells, resulting in a decrease in serum insulin levels. Low serum insulin levels cause reduced glucose uptake into cells.…”

Section: Introductionmentioning

confidence: 90%

Effect of Intermittent Fasting on Muscle Glycogen Stores in Rats with High-Calorie Diet

Kamila¹,

Wiyasihati²,

Lukitasari³

2022

IJRP

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Insulin resistance in skeletal muscle is important since it is normally responsible for most of the insulin-mediated glucose disposal and contains the largest glycogen storage regulated by insulin sensitivity. A high-calorie diet is known to be a risk factor for insulin resistance. Lifestyle modification by regulating caloric intake through intermittent fasting (IF) is recommended to prevent insulin resistance. This study aims to analyze the effect of eight weeks of intermittent fasting on skeletal muscle glycogen stores in rats with a four-week high-calorie diet. A total 24 healthy male Wistar rats were divided into four groups, Ad-libitum with a standard diet (AL-SD); Ad-libitum with a high-calorie diet (AL-HCD); IF with a standard diet (IF-SD); IF with a high-calorie diet (IF-HCD). Muscles were obtained and stained with PAS stain to evaluate glycogen expression based on color intensity. The results of the glycogen expression score were obtained as follows, AL-SD=2.113±0.589; AL-HCD=1.827±0.375; IF-SD=2.109±0.499; IF-HCD=1.979±0.334. Although there was no significant difference between groups (p> 0.05), IF can prevent excessive reduction of glycogen stores in subjects with a HCD compared to the AL-HCD group. In conclusion, this study suggests that IF might improve the reduction of glycogen stores in muscles due to a high-calorie diet.

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Section: Introductionmentioning

confidence: 90%

Effect of Intermittent Fasting on Muscle Glycogen Stores in Rats with High-Calorie Diet

Kamila¹,

Wiyasihati²,

Lukitasari³

2022

IJRP

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“…This study conducted an experimental laboratory study for four weeks (Hoshino et al, 2016;Prasetya et al, 2018;Herawati et al, 2020). The research subjects were 24 female mice (Mus musculus) Balb / C aged ± 8 weeks with a bodyweight of 20-30 g. The mice were acclimatized to the laboratory environment for one week.…”

Section: Subjects and Interventionsmentioning

confidence: 99%

“…All groups received a high-calorie diet every day. The high-calorie was a high glycemic index of 0.0325 ml dextrose 40% per 1 g of mice weight by oral gavage (Herawati, Sari, and Irawan 2020). Intermittent calorie restriction was 50% of the volume of standard, mouse diet given three times/week (Pósa et al 2015;Testa et al 2014).…”

Section: Subjects and Interventionsmentioning

confidence: 99%

The combination of intermittent caloric restriction and moderate-intensity interval training in decreasing blood glucose and CRP levels with a high glycemic index diet

As’ad

Sari

Othman

et al. 2022

KJS publishes peer-review articles in Mathematics, Computer Sci

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This study aims to analyze the effect of the combination of intermittent calorie restriction and moderate intensity interval training (MIIT) on serum c-reactive protein (CRP) levels as one of the inflammatory mediators and blood glucose levels in female mice exposed to a-high glycemic index diet. The sample of this study was female mice Balb/c strain, divided into four groups:, the control group (con), the intermittent calorie restriction group (restrict), the MIIT group (interv), and the combine group of intermittent calorie restriction and moderate-intensity interval training (restrict+interv). A high glycemic index diet was a high calorie and was provided by standard feed and oral gavage of 0.013 g/gBW glucosesolution. Intermittent calorie restriction was 50% reduction of the standard feed 3x/week. The MIIT was five-minute intervals of swimming and 30 seconds of passive rest consecutively for 30 minutes and 3x/week. Meanwhile, the treatment duration was four weeks. The oral glucose tolerance test consisting of fasting blood glucose (FBG) and a two-hour post -prandial (2HPP) blood glucose was measured before and after the treatment. CRP was examined only after the treatment. The restrict + interv group had a significant reduction in CRP levels compared to the restricted group (p = 0.005) and the interv group (p = 0.044). The FBG levels the restrict + interv group and restricted group had a significant decrease (p = 0.026). Moreover, 2HPP of the restrict + interv group and restricted group had a significant decline (p = 0.000). This study concludes that the combination of intermittent calorie restriction and moderate -intensity interval training can attenuate elevated serum CRP and blood glucose levels.

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“…Mice were given a high calorie diet consisting normal amount of food ad libit um per day added with oral gavage of glucose solution with D40 in the amount of 3-5% or 0.0325 ml/gBB (Herawati et al, 2020) every day for 4 weeks. The determination of the oral gavage dose was carried out individually for each mouse by weighing the weight of the mice every week.…”

Section: High Calorie Group Treatmentmentioning

confidence: 99%

Effect of Calorie Restriction on Intestinal Inflammation in Mice Treated with a High-Calorie Diet

Harlinda¹,

Wiyasihati²,

Kurniasari³

et al. 2022

CMUJNS

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Inflammatory bowel disease (IBD) is classified into ulcerative colitis and Crohn's disease. It is chronic inflammation of gastrointestinal tract and idiopathic disease. Sugar is proved to be risk factor for IBD. Calorie restriction is a nutrition intervention to reduce energy intake and can reduce chronic desease. This study aims to determine the difference in the effect of a high-calorie diet and calorie restriction on intestinal inflammation. Eight-week-old healthy female Balb/c mice were divided into two groups. The high calorie (HC) group was fed standard diet plus oral gavage of glucose solution every day for 4 weeks. The dose of glucose solution was 0.0325 ml/gBW or 3-5% additional calories each day. The calorie restriction (CR) group had their calorie intake restricted to 50% of standard diet 3 times a week. After 4 weeks of treatment, the mice had their colon removed. The assessment of the intestinal inflammation was carried out using a light microscope, with a scoring system by Erben et al (2014) in HE staining. The mean score of the HC group was 1.22, this was higher than the CR group with a mean score of 1.18 even tough the result revealed that the scores of intestinal inflammation were not significantly different in the two groups (P = 0.827). However, there is no evidence for the underlying mechanism of intestinal inflammation by high-calorie diet and improvement of intestinal inflammation in high-calorie diet via calorie restriction. Thus, further research is needed on other calorie restriction methods in high-calorie diet study. Keywords: High calorie diet, Calorie restriction, Intestinal inflammation

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High Glycemic Index Diet Decreases Insulin Secretion without Altering Akt and Pdx1 Expression on Pancreatic Beta Cells in Mice

Cited by 10 publications

References 20 publications

Effect of Intermittent Fasting on Muscle Glycogen Stores in Rats with High-Calorie Diet

Effect of Intermittent Fasting on Muscle Glycogen Stores in Rats with High-Calorie Diet

The combination of intermittent caloric restriction and moderate-intensity interval training in decreasing blood glucose and CRP levels with a high glycemic index diet

Effect of Calorie Restriction on Intestinal Inflammation in Mice Treated with a High-Calorie Diet

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