High lactobionic acid production by immobilized Zymomonas mobilis cells: a great step for large-scale process

Carra, Sabrina; Rodrigues, Daniela Cauzzi; Beraldo, Natália Moreno Conceição; Folle, Analia Borges; Delagustin, Maria Gabriele; Souza, Bruna Campos de; Reginatto, Caroline; Polidoro, Tomás Augusto; Silveira, Maurício Moura da; Bassani, Valquíria Linck; Malvessi, Eloane

doi:10.1007/s00449-020-02323-7

Cited by 12 publications

(6 citation statements)

References 25 publications

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“…1 However, the pathogenicity of B. cepacia has prevented its use in commercial LBA production. 17 Z. mobilis coproduces sorbitol owing to the catalytic properties of its lactose-oxidizing enzyme (glucose− fructose oxidoreductase), requiring additional purification step to separate LBA and sorbitol, 18,19 and A. orientalis is a lowproducing microorganism with regard to LBA. 1 P. taetrolens can replace all of these strains because it is nonpathogenic, produces only LBA, and produces it at high levels.…”

Section: ■ Introductionmentioning

confidence: 99%

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Enhancement of Lactobionic Acid Productivity by Homologous Expression of Quinoprotein Glucose Dehydrogenase in Pseudomonas taetrolens

Jang

Lee

et al. 2020

J. Agric. Food Chem.

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This is the first study on improving lactobionic acid (LBA) production capacity in Pseudomonas taetrolens by genetic engineering. First, quinoprotein glucose dehydrogenase (GDH) was identified as the lactose-oxidizing enzyme of P. taetrolens. Of the two types of GDH genes in P. taetrolens, membrane-bound (GDH1) and soluble (GDH2), only GDH1 showed lactose-oxidizing activity. Next, the genetic tool system for P. taetrolens was developed based on the pDSK519 plasmid for the first time, and GDH1 gene was homologously expressed in P. taetrolens. Recombinant expression of the GDH1 gene enhanced intracellular lactose-oxidizing activity and LBA production of P. taetrolens in flask culture. In batch fermentation of the recombinant P. taetrolens using a 5 L bioreactor, the LBA productivity of the recombinant P. taetrolens was approximately 17% higher (8.70 g/(L h)) than that of the wild type (7.41 g/(L h)). The LBA productivity in this study is the highest ever reported using bacteria as production strains for LBA.

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Section: ■ Introductionmentioning

confidence: 99%

“…Z. mobilis coproduces sorbitol owing to the catalytic properties of its lactose-oxidizing enzyme (glucose–fructose oxidoreductase), requiring additional purification step to separate LBA and sorbitol, , and A. orientalis is a low-producing microorganism with regard to LBA …”

Section: Introductionmentioning

confidence: 99%

Enhancement of Lactobionic Acid Productivity by Homologous Expression of Quinoprotein Glucose Dehydrogenase in Pseudomonas taetrolens

Jang

Lee

et al. 2020

J. Agric. Food Chem.

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“…It utilizes the Entner-Doudoroff pathway, which allows fermentation with 50% less ATP production than Embden–Meyerhof–Parnas pathway, leads to a lower biomass yield and a higher uptake rate of sugars when compared to yeasts [ 37 ]. A high level production of native organic acids has been achieved in Z. mobilis [ 38 ] and strains with tolerance to inhibitors found in biomass hydrolysates have been isolated [ 37 ], others being engineered for the same purpose [ 39 ]. The tolerance to inhibitors is an important phenotype when utilizing lignocellulosic biomass hydrolysate, since its pre-treatment generate molecules such as furfural and 5-hydroxymethylfurfural as products of sugar degradation, being acetic acid ubiquitous in this material [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

Engineering Zymomonas mobilis for the Production of Xylonic Acid from Sugarcane Bagasse Hydrolysate

et al. 2021

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Sugarcane bagasse is an agricultural residue rich in xylose, which may be used as a feedstock for the production of high-value-added chemicals, such as xylonic acid, an organic acid listed as one of the top 30 value-added chemicals on a NREL report. Here, Zymomonas mobilis was engineered for the first time to produce xylonic acid from sugarcane bagasse hydrolysate. Seven coding genes for xylose dehydrogenase (XDH) were tested. The expression of XDH gene from Paraburkholderia xenovorans allowed the highest production of xylonic acid (26.17 ± 0.58 g L−1) from 50 g L−1 xylose in shake flasks, with a productivity of 1.85 ± 0.06 g L−1 h−1 and a yield of 1.04 ± 0.04 gAX/gX. Deletion of the xylose reductase gene further increased the production of xylonic acid to 56.44 ± 1.93 g L−1 from 54.27 ± 0.26 g L−1 xylose in a bioreactor. Strain performance was also evaluated in sugarcane bagasse hydrolysate as a cheap feedstock, which resulted in the production of 11.13 g L−1 xylonic acid from 10 g L−1 xylose. The results show that Z. mobilis may be regarded as a potential platform for the production of organic acids from cheap lignocellulosic biomass in the context of biorefineries.

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“…P. taetrolens is one of the most studied LBA-producing bacteria. ,, However, the LBA production ability of this strain was relatively low compared to those of Burkholderia cepacia and Zymomonas mobilis, which are other efficient LBA-producing bacteria. , In our previous studies, we successfully improved the LBA-producing ability of P. taetrolens by optimizing culture conditions and genetically engineering this strain, making P.…”

Section: Introductionmentioning

confidence: 99%

“…9,14,17 However, the LBA production ability of this strain was relatively low compared to those of Burkholderia cepacia and Zymomonas mobilis, which are other efficient LBAproducing bacteria. 18,19 In our previous studies, we successfully improved the LBA-producing ability of P. taetrolens by optimizing culture conditions and genetically engineering this strain, making P. taetrolens more suitable for commercial LBA production. 10,11 Meanwhile, screening of new excellent LBA-producing bacteria can be an alternative approach for economically feasible LBA production.…”

Section: ■ Introductionmentioning

confidence: 99%

Isolation and Characterization of a New Superior Lactobionic Acid-Producing Bacterium, Enterobacter cloacae KRICT-1, from Environmental Soil Samples

Han

Eom

2021

ACS Food Sci. Technol.

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Lactobionic acid (LBA) is a specialty aldonic acid that has been broadly employed in various industries, including food, cosmetics, and pharmaceuticals. In this study, we isolated a new excellent LBA-producing bacterium from soil, designated as strain KRICT-1. A phylogenetic analysis revealed that this strain was identified as Enterobacter cloacae. Previously, we successfully produced LBA using Pseudomonas taetrolens, an excellent LBA-producing bacterium. In a flask culture at 30 °C, E. cloacae KRICT-1 showed the same LBA production concentration (209.3 g/L) and yield (100%) from 200 g/L lactose, compared with those obtained from P. taetrolens cultivated at 25 °C, the growth temperature of which showed the highest LBA production ability. E. cloacae KRICT-1 exhibited an approximately 1.08-fold higher LBA productivity (8.37 g/L/h) than that (7.75 g/L/h) of P. taetrolens, indicating that E. cloacae KRICT-1 was a more efficient LBA-producing bacterium than P. taetrolens. The LBA productivity of E. cloacae KRICT-1 further improved to 8.72 g/L/h at 35 °C. Furthermore, the LBA productivity (9.97 g/L/h) improved at 35 °C in a 5 L fermenter. After whole-genome sequencing of E. cloacae KRICT-1, we found that two quinoprotein glucose dehydrogenases (GDH1 and GDH2) from this strain could produce LBA from lactose by recombinantly expressing these genes in Escherichia coli. Our present results clearly demonstrated that E. cloacae KRICT-1 can be a new promising host for the industrial production of LBA.

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High lactobionic acid production by immobilized Zymomonas mobilis cells: a great step for large-scale process

Cited by 12 publications

References 25 publications

Enhancement of Lactobionic Acid Productivity by Homologous Expression of Quinoprotein Glucose Dehydrogenase in Pseudomonas taetrolens

Enhancement of Lactobionic Acid Productivity by Homologous Expression of Quinoprotein Glucose Dehydrogenase in Pseudomonas taetrolens

Engineering Zymomonas mobilis for the Production of Xylonic Acid from Sugarcane Bagasse Hydrolysate

Isolation and Characterization of a New Superior Lactobionic Acid-Producing Bacterium, Enterobacter cloacae KRICT-1, from Environmental Soil Samples

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