High-level expression by tissue/cancer-specific promoter with strict specificity using a single-adenoviral vector

Kanegae, Yumi; Terashima, Miho; Kondo, Shu; Fukuda, Hiromitsu; Maekawa, Akio; Pei, Zhihua; Saito, Izumu

doi:10.1093/nar/gkq966

Cited by 20 publications

(40 citation statements)

References 39 publications

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“…The former pre-vector lacks both B-box sequences of the VAI and VAII that are essential for the activity of the internal polymerase-III promoter because of the deletions of 15 nt and 17 nt, respectively, (Fig. 1b), and instead bears the FVF fragment at the E4 insertion site near the right end of the viral genome10 (Fig. 1a, upper left).…”

Section: Resultsmentioning

confidence: 99%

“…When the gene product is not deleterious, the titer obtained using this method corresponds to about one fifth of that using either a 50% tissue culture infectious dose (TCID 50 /mL)2526 or a plaque assay; the reason for this difference is probably because the transduction efficiency of 293 cells is much higher than that of other cells. The sequences of TaqMan probes for the titration (named AdV-11012) are derived from Ad5 pIX gene: forward primer, 5′-TGTGATGGGCTCCAGCATT-3′; probe, 5′-ATGGTCGCCCCGTCCTGCC-3′; reverse primer, 5′-TCGTAGGTCAAGGTAGTAGAGTTTGC-3′. A recommended protocol of the titration is available as Supplementary data of reference 12.…”

Section: Methodsmentioning

confidence: 99%

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Efficient production of adenovirus vector lacking genes of virus-associated RNAs that disturb cellular RNAi machinery

Maekawa

Pei

Suzuki

et al. 2013

Sci Rep

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First-generation adenovirus vectors (FG AdVs) are widely used in basic studies and gene therapy. However, virus-associated (VA) RNAs that act as small-interference RNAs are indeed transcribed from the vector genome. These VA RNAs can trigger the innate immune response. Moreover, VA RNAs are processed to functional viral miRNAs and disturb the expressions of numerous cellular genes. Therefore, VA-deleted AdVs lacking VA RNA genes would be advantageous for basic studies, both in vitro and in vivo. Here, we describe an efficient method of producing VA-deleted AdVs. First, a VA RNA-substituted “pre-vector” lacking the original VA RNA genes but alternatively possessing an intact VA RNA region flanked by a pair of FRTs was constructed. VA-deleted AdVs were efficiently obtained by infecting 293hde12 cells, which highly express FLP, with the pre-vector. The resulting transduction titers of VA-deleted AdVs were sufficient for practical use. Therefore, VA-deleted AdVs may be substitute for current FG AdV.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Efficient production of adenovirus vector lacking genes of virus-associated RNAs that disturb cellular RNAi machinery

Maekawa

Pei

Suzuki

et al. 2013

Sci Rep

Self Cite

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“…Accordingly, low level expression of the suicide gene may not be sufficient to activate significant number of prodrug molecules into the toxic forms. To overcome these challenges, construction of chimeric promoters and artificial promoters are currently being investigated which are discussed elsewhere [26, 27] [28, 29]. …”

Section: Cancer Gene Therapymentioning

confidence: 99%

Progress and problems with the use of suicide genes for targeted cancer therapy

Karjoo

Chen

Hatefi

2016

Advanced Drug Delivery Reviews

156

168

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Among various gene therapy methods for cancer, suicide gene therapy attracts a special attention because it allows selective conversion of non-toxic compounds into cytotoxic drugs inside cancer cells. As a result, therapeutic index can be increased significantly by introducing high concentrations of cytotoxic molecules to the tumor environment while minimizing impact on normal tissues. Despite significant success at the preclinical level, no cancer suicide gene therapy protocol has delivered the desirable clinical significance yet. This review gives a critical look at the six main enzyme/prodrug systems that are used in suicide gene therapy of cancer and familiarizes readers with the state-of-the-art research and practices in this field. For each enzyme/prodrug system, the mechanisms of action, protein engineering strategies to enhance enzyme stability/affinity and chemical modification techniques to increase prodrug kinetics and potency are discussed. In each category, major clinical trials that have been performed in the past decade with each enzyme/prodrug system are discussed to highlight the progress to date. Finally, shortcomings are underlined and areas that need improvement in order to produce clinical significance are delineated.

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“…These efforts are described in detail below. The general philosophy of iPlant's development effort is to take advantage of the numerous existing analysis tools and data sets by adapting them to iPlant's foundational CI platform rather than re-developing tools and support systems (Galperin and Cochrane, 2011; Gaudet et al, 2011). (see Box 1 for a summary of the integrated tools.)…”

Section: Collaborations and Grand Challengesmentioning

confidence: 99%

The iPlant Collaborative: Cyberinfrastructure for Plant Biology

Goff¹,

Vaughn²,

McKay³

et al. 2011

Front. Plant Sci.

397

296

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The iPlant Collaborative (iPlant) is a United States National Science Foundation (NSF) funded project that aims to create an innovative, comprehensive, and foundational cyberinfrastructure in support of plant biology research (PSCIC, 2006). iPlant is developing cyberinfrastructure that uniquely enables scientists throughout the diverse fields that comprise plant biology to address Grand Challenges in new ways, to stimulate and facilitate cross-disciplinary research, to promote biology and computer science research interactions, and to train the next generation of scientists on the use of cyberinfrastructure in research and education. Meeting humanity's projected demands for agricultural and forest products and the expectation that natural ecosystems be managed sustainably will require synergies from the application of information technologies. The iPlant cyberinfrastructure design is based on an unprecedented period of research community input, and leverages developments in high-performance computing, data storage, and cyberinfrastructure for the physical sciences. iPlant is an open-source project with application programming interfaces that allow the community to extend the infrastructure to meet its needs. iPlant is sponsoring community-driven workshops addressing specific scientific questions via analysis tool integration and hypothesis testing. These workshops teach researchers how to add bioinformatics tools and/or datasets into the iPlant cyberinfrastructure enabling plant scientists to perform complex analyses on large datasets without the need to master the command-line or high-performance computational services.

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High-level expression by tissue/cancer-specific promoter with strict specificity using a single-adenoviral vector

Cited by 20 publications

References 39 publications

Efficient production of adenovirus vector lacking genes of virus-associated RNAs that disturb cellular RNAi machinery

Efficient production of adenovirus vector lacking genes of virus-associated RNAs that disturb cellular RNAi machinery

Progress and problems with the use of suicide genes for targeted cancer therapy

The iPlant Collaborative: Cyberinfrastructure for Plant Biology

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